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Protein gmlec1b related to fatty acid synthesis and its coding gene and application

A technology for fatty acid synthesis and coding genes, which can be used in applications, genetic engineering, plant genetic improvement, etc., can solve problems such as the inability to effectively improve fatty acid content, achieve broad application and market prospects, and improve the effect of content.

Inactive Publication Date: 2011-12-21
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past ten years, scientists have used biotechnology to genetically improve the key genes in the process of fatty acid biosynthesis, but have failed to achieve significant results. One of the main reasons is that fatty acid synthesis in plants is a complex and highly coordinated process. The process is completed by multiple genes, so improving a single gene for fatty acid synthesis cannot effectively improve the content of fatty acids

Method used

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  • Protein gmlec1b related to fatty acid synthesis and its coding gene and application
  • Protein gmlec1b related to fatty acid synthesis and its coding gene and application
  • Protein gmlec1b related to fatty acid synthesis and its coding gene and application

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Experimental program
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Effect test

Embodiment 1

[0041] Embodiment 1, discovery of gene

[0042] Through homologous sequence comparison, the homologous gene GmLEC1B of Arabidopsis AtLEC1 gene was found in soybean. In the sequence alignment, it was found that the conserved domain of soybean GmLEC1B gene and Arabidopsis AtLEC1 gene reached 95.5% homology.

Embodiment 2

[0043] Embodiment 2, the acquisition of transgenic plants and its detection

[0044] 1. Obtaining of transgenic plants

[0045] 1. Construction of recombinant expression vector

[0046] Soybean (Glycine max cultivar Nannong11382) (the public can obtain from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, the non-patent literature that has recorded this material is Liao Y, Zou HF, Wei W, Hao YJ, Tian AG, Huang J, Liu YF, Zhang JS* and Chen SY*. Soybean GmbZIP44, GmbZ IP62 and GmbZIP78 genes function as negative regulator of ABA signaling and conf er salt and freezing tolerance in transgenic Arabidopsis. Planta. 2008, 228: 225-240) leaf genome DNA was used as a template and the following primers were used:

[0047] GmLEC1B-F: CCctcgagAACGCCTACTTCATCTCTCTTATA (lowercase letter is the restriction site of XhoI) (sequence 5 in the sequence listing) and

[0048] GmLEC1B-R: GGgaattccTATGGAGCGAGCATTTGGTTCATG (lowercase letters are the EcoRI restrict...

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Abstract

The invention discloses a protein GmLEC1B related to fatty acid synthesis, its encoding gene and application. The protein related to fatty acid synthesis provided by the present invention, named GmLEC1B, is derived from soybean (Glycine max L.), and is a protein of the following 1) or 2): 1) consists of the amino acid sequence shown in Sequence 1 in the sequence listing 2) The amino acid residue sequence of sequence 1 in the sequence listing undergoes one or several amino acid residue substitutions and / or deletions and / or additions and is related to plant fatty acid synthesis and derived from 1). After the gene GmLEC1B protected by the invention is overexpressed in Arabidopsis thaliana, the fatty acid content in Arabidopsis seedlings can be increased. This has important theoretical and practical significance for improving the fatty acid content of soybean and related traits, and has broad application and market prospects in the agricultural field.

Description

technical field [0001] The invention relates to the protein GmLEC1B related to fatty acid synthesis in the field of biotechnology, its coding gene and application. Background technique [0002] Vegetable oil has attracted extensive attention as a major edible oil and an important renewable energy substance. At present, 85% of the world's edible oil comes from vegetable oil, and only 25% comes from animals and marine organisms. At the same time, vegetable oil is also widely used in industrial fields, such as detergents, lubricants, paints and biodegradable plastics. In addition, it is worth mentioning that the development of vegetable diesel may provide a new way to solve the global energy crisis and environmental pollution. As people's demand for vegetable oil is increasing, the existing production will be far from meeting the needs of society. [0003] As one of the main economic crops, soybean is an important source of edible vegetable oil. Compared with other vegetabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 梁岩牟金叶左建儒
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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