Protein GmLEC1A related to fatty acid synthesis, encoding gene and application thereof
A technology for fatty acid synthesis and encoding genes, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve problems such as the inability to effectively improve fatty acid content, and achieve the effect of broad application and market prospects
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Embodiment 1
[0039] Embodiment 1, discovery of gene
[0040] Through homologous sequence comparison, the homologous gene GmLEC1A of Arabidopsis AtLEC1 gene was found in soybean. In the sequence alignment, it was found that the conserved domain of soybean GmLEC1A gene and Arabidopsis AtLEC1 gene reached 95.5% homology.
Embodiment 2
[0041] Embodiment 2, the acquisition of transgenic plants and its detection
[0042] 1. Obtaining of transgenic plants
[0043] 1. Construction of recombinant expression vector
[0044] Soybean (Glycine max cultivar Nannong11382) (the public can obtain from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, the non-patent literature that has recorded this material is Liao Y, Zou HF, Wei W, Hao YJ, Tian AG, Huang J, Liu YF, Zhang JS* and Chen SY*. Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis. Planta. 2008, 228: 225-240) leaf genomic DNA is template, with the following primers:
[0045] GmLEC1A-F1: CCctcgagAACGCCTACTTCATCTCTCTTATA (lowercase letter is the restriction site of XhoI) and
[0046] GmLEC1A-R: GGgaattccTATGGAGCGAGCATTTGGTTCATG (lowercase letters are EcoRI restriction sites) for PCR amplification, and the obtained PCR produ...
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