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Protein GmLEC1A related to fatty acid synthesis, encoding gene and application thereof

A technology for fatty acid synthesis and encoding genes, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve problems such as the inability to effectively improve fatty acid content, and achieve the effect of broad application and market prospects

Inactive Publication Date: 2010-12-15
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past ten years, scientists have used biotechnology to genetically improve the key genes in the process of fatty acid biosynthesis, but have failed to achieve significant results. One of the main reasons is that fatty acid synthesis in plants is a complex and highly coordinated process. The process is completed by multiple genes, so improving a single gene for fatty acid synthesis cannot effectively improve the content of fatty acids

Method used

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  • Protein GmLEC1A related to fatty acid synthesis, encoding gene and application thereof
  • Protein GmLEC1A related to fatty acid synthesis, encoding gene and application thereof
  • Protein GmLEC1A related to fatty acid synthesis, encoding gene and application thereof

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Embodiment 1

[0039] Embodiment 1, discovery of gene

[0040] Through homologous sequence comparison, the homologous gene GmLEC1A of Arabidopsis AtLEC1 gene was found in soybean. In the sequence alignment, it was found that the conserved domain of soybean GmLEC1A gene and Arabidopsis AtLEC1 gene reached 95.5% homology.

Embodiment 2

[0041] Embodiment 2, the acquisition of transgenic plants and its detection

[0042] 1. Obtaining of transgenic plants

[0043] 1. Construction of recombinant expression vector

[0044] Soybean (Glycine max cultivar Nannong11382) (the public can obtain from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, the non-patent literature that has recorded this material is Liao Y, Zou HF, Wei W, Hao YJ, Tian AG, Huang J, Liu YF, Zhang JS* and Chen SY*. Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis. Planta. 2008, 228: 225-240) leaf genomic DNA is template, with the following primers:

[0045] GmLEC1A-F1: CCctcgagAACGCCTACTTCATCTCTCTTATA (lowercase letter is the restriction site of XhoI) and

[0046] GmLEC1A-R: GGgaattccTATGGAGCGAGCATTTGGTTCATG (lowercase letters are EcoRI restriction sites) for PCR amplification, and the obtained PCR produ...

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Abstract

The invention discloses a protein related to fatty acid synthesis, an encoding gene and application thereof. The protein related to the fatty acid synthesis provided by the invention is called as GmLEC1A, is derived from soy beans and is the protein shown as 1) or 2): 1) the protein consists of amino acid sequences shown in a sequence 1 in a sequence table; and 2) the protein which is derived from the 1) by substituting and / or deleting and / or adding one or more amino acid residues of amino acid residue sequences in the sequence 1 of the sequence table and is related to vegetable fatty acid synthesis. After performing over-expression on the gene GmLEC1A protected by the invention in arabidopsis, the content of fatty acid in an arabidopsis seedling can be improved. Therefore, the GmLEC1A has important theoretical and practical significance for the improvement on the fatty acid content of the soy beans and the improvement on relative characters, and has board application and market prospects in the field of agriculture.

Description

technical field [0001] The invention relates to protein GmLEC1A related to fatty acid synthesis in the field of biotechnology, its coding gene and application. Background technique [0002] Vegetable oil has attracted extensive attention as a major edible oil and an important renewable energy source. At present, 85% of the world's edible oil comes from vegetable oil, and only 25% comes from animals and marine organisms. At the same time, vegetable oil is also widely used in industrial fields, such as detergents, lubricants, paints and biodegradable plastics. In addition, it is worth mentioning that the development of vegetable diesel may provide a new way to solve the global energy crisis and environmental pollution. As people's demand for vegetable oil is increasing, the existing production will be far from meeting the needs of society. [0003] As one of the main economic crops, soybean is an important source of edible vegetable oil. Compared with other vegetable oils ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11C12P7/64A01H5/00
Inventor 梁岩谭河林左建儒
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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