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Application of caproic acid in inducing resistance of arabidopsis thaliana to Pst DC3000 and method thereof

A technology of Arabidopsis thaliana and hexanoic acid is applied in the application field of hexanoic acid-induced Arabidopsis thaliana resistance to PstDC3000, which can solve the problems of environmental pollution and high cost, achieve less dosage, improve resistance, and do not produce secondary pollution Effect

Inactive Publication Date: 2020-09-04
TAIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps prevent damage caused by oxides from affects crops grown on this type of soil called peaty bogie (a special kind of grass). It involves adding certain substances like caffeine to an agate root system that promotes rapid growth without causing harmful side effects such as reduced nutrients absorption for better healthier roots.

Problems solved by technology

There is technical problem addressed when developing substances called caprolignan or maslinol that help protect plants against various harmful organism attacks like fungus attackers. These compounds may require specific conditions to work properly without causing negative side effects on health caused by certain types of microorganisms.

Method used

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  • Application of caproic acid in inducing resistance of arabidopsis thaliana to Pst DC3000 and method thereof
  • Application of caproic acid in inducing resistance of arabidopsis thaliana to Pst DC3000 and method thereof
  • Application of caproic acid in inducing resistance of arabidopsis thaliana to Pst DC3000 and method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment one: the cultivation of Arabidopsis plant

[0036] The seeds of wild-type Arabidopsis (Col-0) were suspended in 0.1% agarose solution, vernalized at 4°C for 2-4 days at low temperature, and sown in mixed nutrient soil (vermiculite: plant ash: perlite = 6 :2:1), apply fertilizer, dilute the fertilizer and water according to the mass volume ratio l:1000, and cover with film for 2-3 days to facilitate the emergence of seedlings. The culture temperature is maintained at 22-24 °C, the relative humidity is 80%, and the light intensity is about 85 μmol·s -1 m -2 , the light time is 16 hours. Other moisture, insect pests and cultivation management are as usual. 4-week-old Arabidopsis plants were used for the experimental treatments.

Embodiment 2

[0037] Embodiment two: the processing of hexanoic acid

[0038] Prepare 0.6mM hexanoic acid aqueous solution. Use 0.6mM hexanoic acid aqueous solution to carry out root irrigation treatment to Arabidopsis plants, and the same volume of water is carried out root irrigation treatment as a control, and after 2 days, carry out Pst Inoculation of DC3000.

Embodiment 3

[0039] Embodiment three: Pst Culture of DC3000

[0040] Will Pst DC3000 was activated on the solid King's B medium containing 25 mg / L rifampicin in an incubator at 28°C for 2 days. Pick a single colony and place it in a liquid medium containing the same antibiotic at 28°C, 200rpm, and expand the culture until OD 600 =0.6-1.0, then centrifuge at 2500g for 10min to collect the bacteria. The collected cells were treated with 10 mM MgCl 2 Resuspend the solution and adjust the bacterial concentration to OD 600 =0.002 (10 6 cfu / mL), which can be used for inoculation.

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Abstract

The invention discloses application of caproic acid in inducing resistance of arabidopsis thaliana to Pst DC3000 and a method thereof, and belongs to the field of plant resistance induced by compounds. According to the method, after root irrigation or page spraying treatment is conducted on cultivated four-week-old arabidopsis thaliana for two days through a caproic acid aqueous solution, Pst DC3000 is inoculated, and the result shows that arabidopsis thaliana seedlings treated with caproic acid show obviously improved resistance to Pst DC3000. Caproic acid induces arabidopsis thaliana to generate resistance to Pst DC3000 and has the advantages of being good in water solubility, small in usage amount, safe, green, cheap, easy to obtain and the like.

Description

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Claims

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Application Information

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Owner TAIZHOU UNIV
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