Sterile grafting method of Arabidopsis/thellungiella halophila

A technology for Arabidopsis thaliana and salt mustard, applied in the directions of grafting, horticultural methods, botanical equipment and methods, etc., can solve the problems of large differences in the survival rate of grafted bodies, increased difficulty in grafting, and different survival rates of grafting, and achieves low cost. , The method is simple and easy to implement, and the effect is high

Inactive Publication Date: 2017-05-17
SHANDONG NORMAL UNIV
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  • Abstract
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Problems solved by technology

Moreover, the graft survival rate of different species in different periods is also different, even the same species in different periods, the survival rate of the graft body is also very different
[0006] In summary, there are many factors that affect the surv

Method used

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  • Sterile grafting method of Arabidopsis/thellungiella halophila
  • Sterile grafting method of Arabidopsis/thellungiella halophila
  • Sterile grafting method of Arabidopsis/thellungiella halophila

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[0049] Example 1: Aseptic grafting method of Arabidopsis / Salt mustard

[0050] Specific steps are as follows:

[0051] (1) Preparation of rootstocks and scion materials: First, the seeds of Thellungiella halophila (Thellungiella halophila) with full grains in Dongying, China, are disinfected with 0.5% (mass concentration) sodium hypochlorite and sown in agar powder (Sigma agar) concentration (here The "concentration" is the mass fraction of agar in the medium, the same below) is 1.2% 1 / 2MS medium (domestic medium), this process is operated on a sterile ultra-clean bench. After being stratified at 4°C for 1 week, they were placed in a long-day light incubator for vertical cultivation and germination. Use the same disinfection method as salt mustard to disinfect Colombian Arabidopsis (Arabidopsisthaliana) seeds and sown them on 1 / 2MS medium (domestic medium) with agar powder (Sigma agar) concentration of 1.2%. Operate on the bacteria clean bench. Layered at 4°C or directly placed ...

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Abstract

The invention discloses a sterile grafting method of Arabidopsis/thellungiella halophila and fills up the technical blank of related grafting methods in the prior art. According to the technical scheme, the method includes: using a sterile thellungiella halophila seedling 7-10 days after germination as the stock, and using the overground part of a sterile Arabidopsis seedling 3-5 days after germination as the scion; grafting under a sterile condition, placing the grafted seedling on a 1/2MS culture medium with agar powder concentration being 1.2%, performing vertical culture under 18-22 DEG C and short-day illumination, and transferring the grafted seedling into a 1/2hogland nutrient solution for water culture or nutrient soil culture. The sterile grafting method has the advantages that the method is simple and practicable, convenient to operate, capable of reducing pollution, high in survival rate, low in damage and low in cost; the sterile grafting method for different species provides a new thought for the varietal improvement of gardening, fruits, vegetables, and the like, and a basic guarantee is provided for plant grafting in plant molecular mechanism researches.

Description

technical field [0001] The invention relates to the technical field of grafting of small plant test-tube seedlings, in particular to a method for aseptic grafting between the model plant Arabidopsis thaliana and the salt-tolerant model plant Saltina thaliana. Background technique [0002] Grafting is a kind of vegetative reproduction in asexual reproduction, which is carried out by utilizing the wound healing function of plants. As an agricultural production technology, it has been widely used in agricultural, forestry and horticultural plant production and scientific research and development. Since the beginning of the last century, especially in the 1970s and 1980s, people have done a lot of research on the basic theory of grafting. However, some mechanism issues are still unclear. [0003] Arabidopsis thaliana is a small weed in the Brassicaceae family. Because of its short growth period, small individual size, and small genome, it has long been used as a model plant fo...

Claims

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Application Information

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IPC IPC(8): A01G1/06A01H4/00
CPCA01G2/30A01H4/00
Inventor 孙伟李燕孙宇飞谭倩张蘅赵彦修张慧
Owner SHANDONG NORMAL UNIV
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