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Novel high-protein microbial fermentation feed and manufacturing method thereof

A microbial fermentation and high-protein technology, applied in animal feed, animal feed, application, etc., can solve the problems of single effect and inability to use protein feed directly, achieve low cost, improve livestock immunity, and reduce breeding costs Effect

Inactive Publication Date: 2013-04-24
QINGDAO HAIZHONGDA BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the protein microbial fermented feed is mainly processed by a single strain to produce protein, and the effect is single; and because it contains insufficient other energy substances, it cannot be used directly as a protein feed, but only as a feed additive

Method used

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  • Novel high-protein microbial fermentation feed and manufacturing method thereof
  • Novel high-protein microbial fermentation feed and manufacturing method thereof
  • Novel high-protein microbial fermentation feed and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A new type of high-protein microbial fermented feed, which is made by mixing and fermenting the following components according to the weight ratio: 25 parts of worm body, 420 parts of insect sand, 40 parts of bacterial liquid, 20 parts of compound multidimensional, 340 parts of soy sauce grains, and 155 parts of water .

[0034] The compound multidimensional ratio is as follows:

[0035] Vitamin A 20000IU / kg Vitamin D 3 10000IU / kg

[0036] Vitamin E 110mg / kg Vitamin K 3 10mg / kg

[0037] Vitamin B 1 8mg / kg vitamin B 2 12mg / kg

[0038] Vitamin B 6 7mg / kg vitamin B 12 0.2mg / kg

[0039] Biotin 25mg / kg Niacin 120mg / kg

[0040] Calcium pantothenate 60mg / kg Folic acid 6mg / kg

[0041] Copper 3g / kg Iron 3.5g / kg Manganese 2g / kg

[0042] Zinc 3g / kg Iodine 9mg / kg The balance is brewer's yeast powder;

[0043] The bacterial liquid is Pichia jadinii Hzd-pj-003 bacterial liquid, Bacillus subtilis Hzd-bs-001 bacterial liquid and Lactobacillus casei Hzd-lc-002...

Embodiment 2

[0046] The preparation method of a kind of novel high-protein microbial fermented feed described in embodiment 1, concrete process is as follows:

[0047] 1) Preparation of bacterial solution

[0048]A. Preparation of Pichia strains: inoculate the preserved strains into 250mL eggplant bottles filled with 70-80mL solid slant potato medium, and cultivate them in a constant temperature incubator at 30°C for 3 days; Rinse down the bacteria with sterile water, and repeatedly blow and suck to mix the bacteria and sterile water evenly to obtain a bacterial suspension. Put 3% of the inoculum into a 500mL triangular flask containing 300mL of YPD medium, and shake at a constant temperature of 30°C. Bed culture for 24 hours, rotating speed 220rpm; transfer 3% inoculum amount into a 500mL Erlenmeyer flask containing 300mL fermentation medium, and culture on a constant temperature shaker at 30°C for 24 hours, rotating speed 220rpm; Bacterial count≥4.6×10 9 a / mL;

[0049] The formula of...

Embodiment 3

[0063] Fermented feed nutrient composition determination in embodiment 1:

[0064] 1. The crude protein content in this feed is 34.67%.

[0065] 2. Contents of three active substances in the feed: ①The content of free amino acids is 56.21 g / kg. ②The active peptide content is 6.83 g / kg. ③The water-soluble protein content is 12.45g / kg.

[0066] 3. Three kinds of hydrolase activities in the feed: ①Protease activity was 1257.33 U. ②The cellulase activity was 3276.22U. ③The phytase activity was 154.56U.

[0067] test methods

[0068] Determination of protein content

[0069] Crush and sieve the dried sample, weigh 0.5000g into a small Erlenmeyer flask, add 10mL of distilled water, heat to a slight boil in a water bath, maintain for 15min, add saturated CaCl 2 1 mL, 100 g / L KH 2 PO 4 2 mL, after white flocs appear, shake well and filter, then wash the sample on the filter paper with 10 mL of distilled water until the eluate is free of NH 4 + (Tested with Na's reagent), ...

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Abstract

The invention relates to novel cheap high-protein microbial fermentation feed. The high-protein microbial fermentation feed comprises the following components in part by weight: 25 parts of polypide, 420 parts of worm sand, 40 parts of bacterium liquid, 20 parts of composite multivitamin, 340 parts of soy sauce lees and 155 parts of water. The invention has the advantages as follows: the novel cheap high-protein microbial fermentation feed has low cost by using leftovers such as the soy sauce lees and the worm sand as a main matrix; the protein content in the novel cheap high-protein microbial fermentation feed can reach 30-40%; the novel cheap high-protein microbial fermentation feed can be directly fed as an animal diet to substitute 40-60% of complete formula feed, so that the grains are saved and the breeding cost is greatly reduced; and according to the invention, a product fermented by three microorganisms contains higher-content small molecular active substances such as absorbable protein, active peptide and amino acid; and protein and amino acid required in the growing process of livestock and poultry can be effectively supplemented.

Description

technical field [0001] The invention relates to microbial fermented feed, in particular to a novel high-protein microbial fermented feed. Background technique [0002] At present, although the protein content marked in many feeds on the market is high, it includes a large amount of protein in the feed that cannot be truly digested and absorbed by livestock and poultry, and the available protein content is low. In addition, the protein content and energy of fermented feed are not high enough to meet the standard of full-price compound feed, and it is used as functional supplementary feed to feed livestock and poultry. [0003] At present, countries such as the United States, Germany, Britain, France, Canada and Russia are developing rapidly in the research and development of microbial protein fermentation. Various countries have carried out extensive and in-depth research on the characteristics of raw materials, strain selection, fermentation technology, etc. [0004] The r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16A23K1/175
Inventor 栾锦涛陈继伟黄玲玲张盼盼
Owner QINGDAO HAIZHONGDA BIOTECH DEV
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