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Establishment method of animal model in mice infected with sparganosis mansoni

A technology of sparganosis mansoni and animal models, which is applied in the field of medical biology, can solve the problems of hindering the research progress of sparganosis mansoni and not being able to obtain live parasites of sparganosis mansoni at any time, so as to be easy to master and popularize, and to facilitate standardized feeding and management , to avoid the effect of high cross-reactivity rate

Inactive Publication Date: 2010-12-29
ZHENGZHOU UNIV
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Problems solved by technology

The currently used Sparganum mansoni species are mainly collected from the second intermediate host frogs or tadpoles. Due to the limitation of seasonal factors, live Sparganum mansoni cannot be obtained at any time, which seriously hinders the research progress of Sparganum mansoni. hinder

Method used

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Embodiment Construction

[0023] The method for establishing a mouse animal model of sparganosis mansoni of the present invention includes the following steps:

[0024] The first step is to select model animals: select healthy SPF-grade Kunming mice at the age of 2 months for vaccination. There is no restriction on male and female, and fast for 1 day before vaccination;

[0025] The second step, the collection of sparganosis mansoni: capture wild frogs or tadpoles (mainly red frogs or black-spotted frogs, etc.) from nature, and sterilize the sparganosis mansoni from frogs or tadpoles naturally infected with sparganosis mansoni After washing with saline for 3 times, soak in sterilized normal saline;

[0026] The third step, the inoculation of Sparganosis mansoni: Inhale the whole body of Sparganosis mansoni soaked in sterile saline or the cerci with neck (the remaining body can be used to make antigens, etc.) into a gastric lavage device Fix the mouse in one hand, and insert the other hand-held gastric applic...

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Abstract

The invention discloses an establishment method of an animal model in mice infected with sparganosis mansoni, comprising the following steps: cleaning and soaking polypide taken out from the body of the animal naturally infected with sparganum mansoni with sterilized normal saline; injecting the polypide into the gullets of the healthy mice by the method for pouring belly for inoculation; after raising the inoculated mice for a period, transplanting the inoculated mice by the method for pouring belly, carrying out standard raising on the transplanted mice and carrying out one-time new rejuvenation transplantation and collection on the infected mice which are raised for a period within 3-12 months; and establishing the stable animal model in mice infected with sparganum mansoni. The method specifies the collection method of the sparganum mansoni species resources and the living body conservation operation technology, provides the stable disease animal model for the research on immunity characteristics, immunopathological injury, diagnosis and treatment of sparganosis mansoni and provides the standard species resources with stable biological characteristics for the scientific research, teaching, quarantine and diagnostic antigen production units.

Description

Technical field [0001] The invention relates to the field of medical biotechnology, in particular to a method for establishing a mouse animal model of sparganosis mansoni. Background technique [0002] The Ministry of Health conducted a nationwide survey of important human parasitic diseases from 2001 to 2004 and found that the prevalence of parasitic diseases in my country has undergone new changes. The infection rate of food-borne parasitic diseases and zoonotic parasitic diseases Significantly rise. Sparganosis mansoni is just such a food-borne zoonotic parasitic disease, and its incidence is increasing year by year, becoming one of the main factors affecting my country's food safety and people's health. [0003] Sparganum mansoni (sparganum mansoni) is a tapeworm ( Spirometra mansoni ) Larvae can invade the human body and cause sparganosis mansoni. The life history of D. mansoni requires three hosts. The final hosts are mainly cats and dogs. The first intermediate host is Cyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61K49/00
Inventor 崔晶王中全姜鹏李楠王烨王书伟
Owner ZHENGZHOU UNIV
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