Method for breeding a large number of microplitis bicoloratus

A technology of ditch wasp and side ditch, which is applied in the field of insect management, can solve the problems of artificial mass reproduction of double-spot ditch wasp, achieves the effects of improving labor productivity and equipment use efficiency, preventing germplasm decline, and saving costs

Inactive Publication Date: 2010-12-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the deficiencies in the prior art, to solve the problem of artificial mass reproduction of Braconus bispotae, to reproduce Braconus bispo...

Method used

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Examples

Experimental program
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Embodiment 1

[0021] 1. Host breeding: use beet armyworm or Spodoptera litura larvae from the end of the 2nd to the beginning of the 3rd instar as the intermediate host, including host egg collection, egg hatching, larva rearing, and adult feeding;

[0022] The host eggs were hatched at a constant temperature of 25°C. After hatching, the larvae were raised in plastic square boxes (4cm high, 16cm long, and 16cm wide) filled with artificial feed. The breeding density was 200 worms / box, and the feeding conditions: 25-27 °C, RH=60-70%, photoperiod L:D=12:12, light intensity 500LUX. It can be provided to parasitic wasps for parasitism at the end of the 2nd age to the beginning of the 3rd age.

[0023] Host adults are placed in sarongs, fed with 10% honey water, and allowed to lay eggs on the ovum-laying paper.

[0024] 2. Receiving bees: according to the ratio of parasitic bees: host number of 1:5-10, use self-made plexiglass cages for inoculation. The plexiglass cages are 28cm long, 28cm wide,...

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Abstract

The invention relates to a method for breeding a large number of microplitis bicoloratus for controlling noctuidae injurious insects, belonging to the field of insect management. Beet armyworm or prodenia litura larvas between the end of second instar and the beginning of third instar are used as intermediate hosts, and microplitis bicoloratus is used as parasitic wasps for wasp inoculation, wherein the quantity ratio of the parasitic wasps to the hosts is 1:5-10. The hosts are bred under the conditions that temperature is 25-27 DEG C, RH (Relative Humidity) is 60-70%, photoperiod L:D is 2:12 and light intensity is 500 LUX. The parasitic wasps come out of polypide for cocooning, pupation and eclosion to obtain the microplitis bicoloratus. The invention realizes the efficient breeding of a large number of high-quality graparasitic wasps, namely microplitis bicoloratus which can be applied to the control of prodenia litura; the invention provides a solid basis for applying the microplitis bicoloratus to the control of the noctuidae injurious insects in large lands. The method is not limited by seasons, ensures the long-time abundant supply source of parasitic wasps and is beneficial to the long-time efficient control of noctuidae.

Description

technical field [0001] The invention belongs to the field of insect management, and relates to a single larval endoparasitic wasp in nature, in particular to a bispotted wasp that can be used to prevent and control Noctuidae pests in fields and vegetables. Background technique [0002] Noctuidae pests are widely distributed in subtropical and temperate regions of the world. In China, vegetables, fruits, rice, and garden plants in most areas are all harmed by Noctuidae, especially Spodoptera litura and Spodoptera litura, causing serious economic losses. Microbial insecticides and parasitoids are important biological factors in integrated pest management of these crops. Among them, Spodoptera litura polyhedrosis virus insecticides and beet armyworm polyhedrosis virus insecticides have been commercialized (Zongwen No. 1 and Chongwen No. 2), and are widely used in the control of these crop pests. [0003] Among IPM strategies, parasitic wasps have attracted much attention beca...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 范静包伟罗开珺李广宏庞义
Owner SUN YAT SEN UNIV
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