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A kind of method of golden wasp controlling American white moth

A technology of American white moth and golden bee, applied in invasive species monitoring, climate sustainability, animal husbandry, etc., can solve the problems of low success rate of replacement hosts and unstable bee numbers, and improve labor productivity. and equipment use efficiency, reducing artificial reproduction costs, and the effects of low reproduction costs

Active Publication Date: 2022-07-12
SHENYANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to make up for the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for preventing and treating the white moth of the American bollworm by using alternative hosts, the chrysalis chrysalis, to reproduce the cotton bollworm A. The problem of low rate and unstable number of breeding bees, and increase the similarity between the offspring of the cotton bollworm golden wasp obtained by breeding and its natural breeding offspring, so as to ensure the parasitic ability of the cotton bollworm golden wasp to the American white moth. Enrich the dominant parasitoid species of American white moth control, and add new means and new methods for the biological control of American white moth

Method used

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  • A kind of method of golden wasp controlling American white moth
  • A kind of method of golden wasp controlling American white moth
  • A kind of method of golden wasp controlling American white moth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 The influence of the breeding methods of different yellow-pink beetle pupae on the effect of docking bees

[0030] Cultivation method of yellow-pink beetle pupae Ⅰ: The method of breeding Helicoverpa armigera golden wasp by gradient cooling, yellow-pink beetle is from the subcultured population in the laboratory. Placed at a temperature of 22-28° C., a relative humidity of 50-70%, and a photoperiod of L:D=16h:8h; subcultured in a rearing box with feeds such as wheat bran, vegetables and fruits. In the pupation stage, the above-mentioned yellow powder armor pupae are transferred from 22-28 ℃ into the insect raising box with ventilation holes and then carry out the gradient cooling treatment; the gradient cooling treatment is: placed in a 16 ℃ incubator and placed for 2 hours , and then placed in a 10°C incubator for 2 hours to obtain yellow-pink bee pupae for receiving bees, which were stored in a 4°C incubator in the dark for 1-20 days, so that all pupae we...

Embodiment 2

[0038] Example 2 Comparison of the effects of different alternative hosts for breeding Helicoverpa armigera

[0039] 1. Host Screening Assay

[0040](1) The pupae of American white moth, which were collected in the field and parasitized by Helicoverpa armigera, were placed under the conditions of temperature of 22-28°C, photoperiod L:D=16h:8h, and relative humidity of 50-70%. , the feed is honey water with a concentration of 5%, and cultivated until the bees emerge;

[0041] (2) put into the bottom of the 10ml centrifuge tube with the yellow powder beetle pupae (see the breeding method Ⅰ of the yellow powder beetle pupae of embodiment 1) for the breeding method; the Helicoverpa armigera female bee is connected to the centrifuge tube, the golden wasp female bee and the substitute host The quantity ratio is 3:1, place a piece of absorbent cotton ball soaked in 5% honey water at the upper inner wall of the centrifuge tube, pick up the bees after 48 hours, remove the adult bees i...

Embodiment 3

[0047] The influence of embodiment 3 passage breeding on the cotton bollworm golden wasp

[0048] According to the breeding method of Tenebrio molitor pupa through gradient cooling method (treatment 1), control 1, control 2, and control 3, 4 generations were continuously bred respectively, and the above-mentioned 1st generation and 3rd generation of the golden wasp were counted. The eclosion rate and male-to-male ratio.

[0049] Table 3 compares the effect of raising this golden bee by passage

[0050]

[0051] The data in Table 3 show that after 6 consecutive generations of breeding of Tenebrio molitor pupa by gradient cooling, the species emergence rate of Helicoverpa spp. was improved, but the sex ratio did not change significantly.

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Abstract

The invention relates to a method for preventing and controlling the American white moth by the golden wasp, which is characterized in that: the golden wasp is the cotton bollworm golden bee, and the control method comprises the following steps: (1) rearing the golden cotton bollworm adult; ( 2) Cultivate the yellow-pink beetle pupae, transfer the yellow-pink beetle larvae into an insect-raising box with ventilation holes, and then carry out a gradient cooling treatment, and place the treated yellow-pink beetle pupae in a 4°C incubator for protection from light to obtain a bee-receiving use. The yellow-pink beetle pupa; (3) receiving the bees, the step (2) the yellow-pink beetle pupa is reversely heated to receive the bee, and the ratio of the yellow-pink beetle pupa to the number of female adult bees of Helicoverpa armigera is 1:3; (4) parasitic Management of bee development; (5) release of parasitic wasps. The method adopts the alternative host yellow powder beetle pupae to breed Helicoverpa armigera on a large scale indoors, solves the problems of low success rate of the alternative host and the unstable number of breeding bees, and at the same time enriches the dominant parasitic wasp species in the control of American white moth, which is the most important in the United States. The biological control of white moth adds new means and new methods.

Description

technical field [0001] The invention relates to the technical field of biological pest control, in particular to a method for preventing and controlling the American white moth by using the cotton bollworm golden wasp. Background technique [0002] Hyphantriacunea is a worldwide quarantine object and an important invasive species in my country. It originated in North America and was later introduced to Eurasia. The American white moth has the characteristics of strong adaptation and reproduction ability, wide adaptation range, difficult control and fast transmission. At present, the commonly used species in biological control is the white moth Chouioiacunea, which can use the tussah silkworm in production. The artificial breeding of pupae has played an important role in the control of American white moth. However, at present, American white moth is still spreading and its distribution area continues to expand. It is necessary to develop and utilize more dominant natural ene...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033Y02A90/40
Inventor 孙守慧张艺馨史俊瑞杨丽元
Owner SHENYANG AGRI UNIV
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