70results about How to "Reduce reproductive costs" patented technology

The invention discloses an in vivo reproduction method of entomopathogenic nematodes, relating to an in vivo culture method of entomopathogenic nematodes and aiming to solve the problems of low yield in applying the tenebrio molitors to breed the nematodes. The in vivo reproduction method comprises the following steps of: firstly, cutting two pieces of medium speed qualitative filter paper and filling in a cover of a culture dish A; then placing prodenia litura larvae; then soaking the medium speed qualitative filter paper by using water suspension infective juveniles; covering the bottom of the culture dish A to the cover of the culture dish A till all prodenia litura larvae die; removing the bottom of the culture dish A; placing the cover of the culture dish A in a culture dish B; filling sterile water to a gap between the culture dish B and the culture dish A; culturing at a constant temperature till the infective juveniles occur in the sterile water; then collecting the sterile water with the infective juveniles every day; and complementing the sterile water so as to complete the in vivo reproduction of the entomopathogenic nematodes. The in vivo reproduction method of the entomopathogenic nematodes is mainly used for the in vivo reproduction of the entomopathogenic nematodes.

The invention discloses a Paris polyphylla var yunnanensis and paris polyphylla var chinensis seedling three-phase breeding technology comprising the following steps: 1, seed selection; 2, seed pretreatment: selecting cleaned rude river sand to rub the seeds until a seed outer red aril falls off, the rubbing process cannot be too long to cause the inner layer seed surface to damage, and carrying out low temperature treatment, changing temperature treatment, normal temperature treatment and constant temperature water immersion treatment for the seeds removing arils, and reserving the seeds for usage; 3, embedding and accelerating germination: uniformly sowing the immersed Rhizoma Paridis seeds, watering the seeds for moisture, and carrying out embedding accelerating germination treatment for a watered sowing basket; 4, transplanting the emerged seedling. The novel technology can effectively reduce Rhizoma Paridis seed seeding formation time; after seed pretreatment, embedding accelerating germination and transplanting seedling, the emergence rate of the Paris polyphylla var yunnanensis and paris polyphylla var chinensis can respectively reach 70% and 80%, thus greatly reducing time cost for production and resource large scale utilization, and improving efficiency.

The invention discloses a novel method for improving catalpa bungei cuttage propagation efficiency.The method includes the first step of construction of a cutting orchard, the second step of preparation of a cuttage seedbed, the third step of preparation of a cuttage matrix, the fourth step of cultivation and harvesting of cuttings, the fifth step of cuttage, the sixth step of daily management after cuttage, and the seventh step of seedling hardening and transplanting.According to the transpiration and rooting rules of plants, tender branches low in lignification degree are obtained in a shading mode, cuttings are subjected to shading treatment through a shading method, the catalpa bungei cuttage survival rate is greatly increased and as high as 92%, inconvenience caused by catalpa bungei seedling propagation by means of tissue culture, grafting and other technologies is overcome, nursery cultivation time is greatly shortened, operation is easy, and the method is convenient to control and good in practicality.

The invention discloses a photinia lochengensis leafbud cutting seedling raising method. The method comprises the following steps that (1) proper cutting time is selected; (2) a cutting medium is prepared; (3) a rooting agent is prepared; (4) cutting slips are treated and cuttage is conducted; (5) management and transplantation are conducted after cuttage. According to the photinia lochengensis leafbud cutting seedling raising method, the cutting survival rate is over 95.6% and cost is reduced by 50% to 80% compared with that of a photinia lochengensis branch cutting method and only accounts for about 15% of cost of a tissue culture method. The photinia lochengensis leafbud cutting seedling raising method has the advantages that operation is easy and convenient, cost is low, the technical requirement is simple, popularization and application are easy, and the method has important application and popularization value in rapid propagation production of photinia lochengensis seedlings.

The invention provides an efficient breeding method of purple potato tissue culture seedlings. The efficient breeding method of the purple potato tissue culture seedlings comprises the following steps of treating purple potato tubers at ultralow temperature; performing induced culture on root tips and stem tips of seed potatoes; performing rooting culture on strong seedlings, and conducting virus detection on test-tube plantlets; performing induced culture and virus detection on miniature potatoes; performing transplanting and cuttage grafting breeding on the miniature potatoes. According to the efficient breeding method of the purple potato tissue culture seedlings, provided by the invention, miniature potato seedlings are bred by adopting virus eradication of stem tips and root tips; the culture cycle of the root tips is different from that of the stem tips; the root tips and the stem tips are jointly cultured, so that the time cost can be effectively reduced, seed potato resources are fully and efficiently utilized, and further the reproduction cost of the tissue culture seedlings is reduced; besides, the test-tube plantlets cultured by using the root tips are high in reproduction speed, so that the working efficiency is improved, and the production cost is reduced.

The invention provides a method for high-density cutting seedling of twigs of Lagerstroemia indica Pink Velour. The method comprises the following steps: (1) selecting cutting medium; (2) selecting cutting slips; (3) cutting; (4) managing after cutting; (5) hardening seedlings and transplanting. Compared with the prior art, the method provided by the invention not only has the advantages of simple condition, easy operation and low reproductive cost in the prior art, but also can increase the average root length of cut lagerstroemia indica obviously and can greatly increase the survival rate of transplanted seedlings.

The invention discloses a camellia grafting and propagating method. The method is characterized in that the grafting time is between the middle of May and the middle of June; a rootstock is a camellia germ with a length of 6 cm which is not lignified; and a scion is a semi-lignified or lignified spring twig which is at the middle upper part of a camellia tree, has full bud and is free from pest and disease damage. The camellia grafting and propagating method comprises the following steps: cutting from the 2 cm position of the camellia germ and then longitudinally cutting from the center of a fracture by using a thin blade, wherein the cutting length is 1 cm; and cutting a leafy bud with a length of 1.5 cm at the lower end and a length of 0.5 cm at the upper end from the scion, cutting the lower part of the bud into a wedge-shaped surface with a length of 1 cm and cutting a half of leaf and immediately inserting into the rootstock after the completion of cutting, so that the half of leaf and the rootstock are aligned with each other and are tightened. A grafted seedling is cultivated onto a sandy seedling bed, and 0.5 percent of urea aqueous solution or 0.1 percent of monopotassium phosphate is applied every 10 days.

The invention discloses a method for breeding aconite which comprises the following steps of: soaking seed with GA3 solution, sowing seeds, managing after sowing, transplanting, and the like. Before the aconite seed is sown, the aconite seed is soaked in a GA3 solution having a concentration of 400mg / L to 1000 mg / L, in order to obviously promote the germination percentage and the germinating energy of the aconite seed, increase the fresh weight of the over-ground part of the aconite seedling, and emerge rapidly and orderly. The growing of the aconite seedling can be boosted and the earthnut diameter can be increased by using a proper sowing and managing method so as to decrease the breeding cost of the aconite and promote the yield of the aconite.

The invention provides a plumbago auriculata shoot cutting propagation method. The method comprises the following steps: (1) selecting a cutting substrate; (2) selecting a cutting shoot; (3) treating by a rooting agent; (4) performing the cutting; (5) managing after the cutting. Compared with the prior art, the plumbago auriculata shoot cutting propagation method not only has the advantages of the prior art such as simple conditions, easy operation and low propagation cost, but also can remarkably increase the average main root length of the cut plumbago auriculata, and can greatly improve the survival rate.

The invention discloses a plant-animal-combined efficient heavy-metal-contaminated soil repairing method. Through large-scale earthworm propagation, earthworm cocoons are produced in a batched manner, and the situation that a batch of the earthworm cocoons die after being inoculated into the soil directly due to high-concentration pollution is prevented. Plants with green seedlings planted in the earlier period serve as the feed, the earthworm propagation cost is lowered, and the requirements for a stocking area are lowered; the plants and earthworm breeding devices are arranged in a strip-shaped manner at intervals, meanwhile the earthworm breeding devices are convenient to place, and the earthworm cocoon processing difficulty is lowered; and meanwhile, the biodegradable plant fibers are arranged and do not need to be recycled, processing efficiency is improved, and large-scale high-efficiency processing is facilitated in the meantime. A separating conveyor belt is utilized to recycle earthworms, the processing efficiency of the soil is greatly improved, and the separation difficulty and cost are lowered. The earthworm breeding devices are provided with earthworm sustained-release capsules, the situation that a batch of the earthworms die at the same time is prevented, sustained-release is performed according to the sustained-release, and gradient repair is achieved.

The invention relates to a method for propagating Japan exorista japonica with wax moth. The method comprises the steps of putting 60 Japan exorista japonica pupas which are pupated in the same day ina raising cage; placing wax moth larvae treated with 75% alcohol and hot water of 60 DEG C with a culture dish, and placing the culture dish in the raising cage; taking out the culture dish each 2h,moving the wax moth larvae on which the exorista japonica lays eggs, to a disposable plastic cup, wherein 50 exorista japonica pupae are placed in each cup; after 15-20d, taking out the exorista japonica from the disposable transparent plastic cup, and integrally placing the exorista japonica in a new disposable transparent plastic cup, wherein 60 exorista japonica pupae are placed in each disposable transparent plastic cup; and after the exorista japonica are subjected to emergence, moving the disposable transparent plastic cup into a new exorista japonica collecting cage, and starting new round of raising and propagating. The host wax moth larvae used by the method are easy to obtain, simple to operate, and low in cost for raising Japan exorista japonica.

The invention discloses a method for increasing bumblebee commodity group propagation speed. The method comprises the following steps: preparing bee species and queen bee, preparing propagation environment and feed, inducing the queen bee to lay eggs, performing transfer box feeding to obtain a bumblebee commodity group, packaging the bumblebee commodity group, and adding worker bees into a feeding box in which the queen bee lives after inducing the queen bee to lay eggs and before performing transfer box feeding. The method for increasing bumblebee commodity group propagation speed provided by the invention shortens the time for propagating single queen bee into the commodity group to 55-65 days, which is shortened by 15 days in comparison to the ordinary propagation time of 70-80 days; the propagation cost is reduced by 20%.

The invention discloses a cutting and breeding method for tree stocks of Populus tremula * P. tremuloides, comprising the following steps: preparing a slotter, manufacturing cuttingwood, processing, cutting, managing after cutting, transplanting and planting in field, wherein in the step of manufacturing the cuttingwood, the current-year semi-lignified twigs of the Populus tremula * P. tremuloides are collected from March to May every year to serve as cutting, simple-ink single-leaf stalk sections are cut from the cuttings to serve as the cuttingwood. When the method of the invention is adopted for cutting and breeding the tree stocks of the Populus tremula * P. tremuloides, callus grows on the cuttings one week after cutting; roots begin to grow after 12 days; and after 18 days, the average length of the roots reaches 6.5cm, the average diameter of the roots reaches 0.18 cm, and the rooting rate reaches above 80%. The method of the invention improves the cutting survival rate of the Populus tremula * P. tremuloides to a greater degree; and in addition, the method of the invention also has the advantages of short emergency time, high rooting rate, simple and easily controlled technology, low breeding cost and the like.

The invention relates to a method for the propagation and rejuvenation of scleroderma guani Xiao et Wu with Chinese chestnut weevils as intermediate hosts and the use thereof and belongs to the technical field of insects. Mature larvae of Chinese chestnut weevils, as hosts, and mated scleroderma guani Xiao et Wu females, as breeding bees, are put in a culture container, wherein the ratio of the number of the breeding bees to the number of the hosts is 1-3:1; culture is carried out for 1-2 days with the culture container having a temperature of 25-27 DEG C and a humidity of 55-70%; after the breeding bees oviposit, scleroderma guani Xiao et Wu goes through the larval phase and the pupal phase and grows to adults. The breeding bees can be scleroderma guani Xiao et Wu which has degenerated after breeding for 4-5 generations in other hosts. The use of the method is that the obtained scleroderma guani Xiao et Wu adults can perform parasitic propagation in stem borers and thus kill the stem borers. Chinese chestnut weevil larvae are used as substitute hosts for the propagation of scleroderma guani Xiao et Wu and inducing hosts are not needed, so that the breeding cycle of scleroderma guani Xiao et Wu is greatly shortened, steps are simplified and the breeding cost is reduced; the utilization rate of Chinese chestnut weevil larvae, as substitute hosts, is higher than that of mature larvae of corn borers and pupae of tenebrio molitors.

The invention discloses a tissue culture rapid propagation method for excellent sequoia sempervirens seedlings. The method comprises the following steps: constructing explants, disinfecting the explants, performing differential induction, performing enrichment culture, performing ex vitro rooting, transplanting and cultivating. An excellent clone branch of robust annual sequoia sempervirens is selected and is cut into 2-3cm long stems with buds as the explants for tissue culture rapid propagation. The tissue culture rapid propagation method for excellent sequoia sempervirens seedlings disclosed by the invention is slightly influenced by the external environment, is capable of greatly reducing the propagation cost, shortening the propagation period, increasing the propagation coefficient ofthe seedlings and acquiring the seedlings in highly consistent quality, is convenient for large-scale factory cultivation and has important significance in the aspects of seedling propagation and value utilization of the excellent sequoia sempervirens clone in the future.

The invention discloses a cutting rapid propagation method for angelica keiskei and belongs to the field of agricultural technologies. The cutting rapid propagation method for the angelica keiskei is used for simplifying the propagation technology of the angelica keiskei, shortening the propagation period, reducing rapid propagation cost and achieving rapid propagation of the angelica keiskei. The method is mainly used for the medicinal and edible angelica keiskei and includes the detailed steps that stems with the length of 3 cm-5 cm and the thickness of 0.2 cm-0.8 cm are selected and provided with 2-5 compound leaves and 2-5 buds, then the stems are rapidly dipped into rooting powder with 200-400 diluents for 15 seconds-30 seconds, cut into wet sand and watered with sufficient water, under the conditions that the temperature is (25+ / -2) DEG C, the air relative humidity is over 75%, the illumination is 10,000 lx-20,000 lx, and the illumination time is 12 h / d-14 h / d, the stems are cut for 7-15 days, the survival rate is 100%, seedlings which are survival through cutting are transplanted into a land sun-shade shed, the spacing in the rows and the spacing between rows are 20 cm*20 cm, the temperature is 25 DEG C-30 DEG C at daytime, the temperature is 15 DEG C-18 DEG C at night, the air relative humidity is 70%-90%, the shed is appropriately opened for ventilation, and the survival rate reaches 100% at the seventh-tenth day after transplanting. Compared with a tissue culturing rapid propagation technology, the method simplifies the propagation steps, shortens the propagation time and reduces propagation cost, and application and popularization are facilitated.

The invention relates to a rapid breeding method for the tissue culture of menispermum dauricum. The method comprises the steps of obtaining aseptic seedlings of the menispermum dauricum so as to obtain cluster buds, carrying out multiplication on the cluster buds to obtain seedlings, strengthening the seedlings, carrying out field root induction, and the like. According to the method, the aseptic seedlings are obtained by adopting methods of sterile sowing and the tissue culture, and the steps of strengthening the seedlings, carrying out rooting culture and transplanting are carried out, so that the method is low in breeding cost and short in cultivation period; furthermore, the method is simple and easy to operate and convenient to popularize, and the technical support is provided for development and utilization as well as rapid propagation of high-quality varieties.

The invention discloses a method for reproducing sowing of liana wintercreeper branch sections. The method includes the steps of (1) selecting one-year and sturdy wintercreeper branches as cuttings; (2) shearing (cutting) each cutting obtained in the step (1) via branch scissors or a fodder chopper into a branch section which is 5-10 centimeters in length and provided with more than two leaf burls; (3) sowing the branch sections obtained in the step (2) in a seedbed, covering 1-2 centimeters of nutrient soil, and watering thoroughly; and (4) covering moisture preserving material on the seedbed sowed with the wintercreeper branch sections so as to preserve moisture for the seedbed. In late spring, summer and early autumn, the wintercreeper branch sections can grow into new plants after 1-2 months, and in early spring and late autumn, the wintercreeper branch sections can grow into the new plants after 2-3 months. The method does not need to pay attention to biological upper and lower ends, is simple to operate, high in efficiency and low in reproducing cost.

The invention discloses yellow-head catfish meat semen preserving fluid and a preparation method thereof. The yellow-head catfish meat semen preserving fluid is characterized by being prepared through the following steps: mixing 0.5-1.5% of trisodium citrate, 1.5-3.5% of glucose, 0.1-0.3% of sodium bicarbonate, 0.02-0.04% of potassium chloride and deionized water in percentage by mass volume, putting the mixture into a magnetic stirrer, stirring for 3-15 minutes, and determining the volume after sufficiently dissolving. Tests show that if yellow-head catfish meat semen is preserved in the preserving fluid for 8 hours at normal temperature, 100% of sperms have the activity after being activated, and the fertility rate is 98% when the sperms are used for fertilization; if the semen is preserved for 14 days at the refrigeration condition of 4 DEG C, 100% of the sperms have the activity, and the fertility rate is 95% when the sperms are used for fertilization.

The invention mainly aims at providing a cabbage type rape parent seed multiplication method which can not only ensure purity but also realize higher multiplication yield and lower cost. The method comprises the following steps of: sowing rape parent seeds in March, and controlling the growth temperature at 15-25 DEG C; transplanting in April, selecting the region with the altitude of above 1000m in Yangtze River valley in the south of Qinling-Huaihe Line in China as a transplanting region, adopting a plastic film for coverage, and breaking the film for transplanting; and enabling progenitive rape parents to bloom in May-June and mature in July-August. According to the invention, the high-altitude low-temperature region of the Yangtze River valley is substantively selected, the sowing is performed in spring, and a means for promoting growth is simultaneously adopted, so that the growth and development of plants can be ensured, and simultaneously, the flowering phase of the plants can be significantly staggered with the flowering phases of similar plants, thus the mixing can be avoided. The parent seed multiplication has good economy and high yield, and the method provided by the invention has extensive popularization and application values.

The invention belongs to the field of biotechnology breeding, and specifically relates to a wild cabbage BoMS1 gene and an application thereof to preparation of sterile materials. The invention provides a wild cabbage BoMS1 gene namely a key gene for controlling wild cabbage males to be sterile, the expression of the wild cabbage BoMS1 gene is lowered through an RNAi technique, and further a genetically modified male sterile plant system is prepared, so that the problem that heredity weakness risk exists because single ogura CMS single sterile resources are massively depended when wild cabbagefirst filial generation variety breeding is performed in a male sterile way is solved. The expression of the wild cabbage BoMS1 gene and the expression of a wild cabbage SRK gene are lowered throughthe RNAi technique, and the genetically modified male sterile system which is compatible with blossoming hybridization of a maintenance line is prepared. Through the adoption of sterile strains prepared according to the method, the problem that the propagation of the maintenance line needs artificial bud pollination is solved, and the propagation cost of wild cabbage male sterile systems is lowered; a non-genetically modified male sterile system which is compatible with blossoming hybridization of the maintenance line is further prepared through simultaneous mutation of the wild cabbage BoMS1gene and the wild cabbage SRK gene.

The invention discloses a leaf bud cutting and seedling raising method of cotoneaster multiflorus. The leaf bud cutting and seedling raising method comprises the steps of preparing a cutting base material and a rooting agent, preparing shoots for cutting, treating the shoots for cutting, cutting and the like. The method disclosed by the invention is simple and convenient to operate and feasible, is low in cost and has simple technical requirements.

The invention relates to a circular flower cultivation container, and belongs to the technical field of flower planting. The circular flower cultivation container includes a circular bottom, and a stretchable cylindrical wall which is arranged on the circular bottom; the circular bottom is provided with a water hole; the cylindrical wall is provided with a plurality of wrinkles; and the wrinkles form grooves or bosses which are parallel to each other and pass through the circular wall. The circular flower cultivation container can automatically adjust the size of the container, can meet the demands of the root system growth condition and fertilizer and soil during different stages of flowers, can avoid container-changing, and can save the cost of flower reproduction.

The invention discloses a method for culturing aphidiidae by using potatoes as hosts. The method comprises the following steps: culturing host potato seedlings, transplanting the potato seedlings to a potato aphid room, inoculating 10-11 potato aphids to true leaves of each potato plant when 5-6 true leaves of each potato plant grow up, enabling the potato aphids to spread and reproduce naturally, moving the potato seedlings into an aphidiidae room, inoculating aphidiidae into the aphidiidae room, and promoting the aphidiidae in the aphidiidae room to lay eggs for parasitism inside the potato aphids, thereby obtaining stiffened potato aphids. By adopting the method that potatoes are adopted as the hosts to culture aphidiidae, the reproduction cost can be greatly lowered when being compared with that of a method that cigarette seedlings are adopted as the potato aphid hosts since the potatoes are low in cost, rapid to grow and high in potato aphid reproduction rate.

The invention relates to the technical field of planting and discloses a treatment method capable of improving germination ability of hibiscus hamabo. The treatment method includes: soaking, vacuum heating, blending seeds, and accelerating germination. The method is simple, convenient to operate and needless of high-temperature treatment, hibiscus hamabo seeds can germinate after 8-9 days of germination accelerating, and germination rate reaches 53.7%, so that propagation efficiency of the hibiscus hamabo seeds is improved obviously, and propagation cost is saved greatly. The seeds are put ina compound enzyme solution for soaking to damage tissue structure of seed husks, so that the seeds are promoted to absorb outside nutritional ingredients, and growth of radicle and germ is promoted; the seeds after being soaked are put into a vacuum rotary evaporator for vacuum heating to damage activity of dormant substances, so that seed dormancy is broken, seed protoplasm activity is improved,and seed germination is accelerated; a seed dressing agent is added into the seeds after vacuum heating, nutrition is rich, molding and rotting of the seeds are avoided, growth of the seed radicle andgerm is promoted, seed germination is accelerated, and seed propagation efficiency of hibiscus hamabo is improved.

The invention relates to the technical field of lonicera caerulea propagation, in particular to a lonicera caerulea twig cutting medium seedbed and a cutting propagation method. The lonicera caeruleacutting medium seedbed comprises a substrate layer and a surface layer, wherein the surface layer is positioned on the surface of the substrate layer; the substrate layer comprises fine river sand, larch needles, sawdust and garden soil, and the volume ratio of the fine river sand to the larch needles to the sawdust to the garden soil is (1.8-2.2): (0.4-0.6): (0.4-0.6): (0.8-1.2); and the surfacelayer comprises larch needles and saw dust, and the volume ratio of the larch needles to the saw dust is (0.4-0.6): (0.4-0.6). When the cutting medium seedbed is used for lonicera caerulea cutting propagation, the cutting medium seedbed is good in moisture retention performance, high in nutrient, high in rooting rate and survival rate, capable of being directly used for bed reserving for overwintering (high in preservation rate), low in cost, simple in raw material, suitable for factory-like seedling propagation and particularly beneficial to seedling strengthening and increasing of the survival rate in the bed moving and seedling hardening process.

The invention relates to the technical field cedar planting, in particular to a cedar pollen tissue culture propagation method. The method includes steps: impregnating fruit balls into silver iodide aqueous solution to realize ultrasonic sterilization; performing inoculation of the sterilized fruit balls in a culture medium to realize root and germination induction, wherein the culture medium is formed by an MS culture medium, 1-1.2mg / L epsilon-polylysine, 0.2-0.3mg / L sodium pyrophosphate and 1.8-2mg / L divalent calcium ions; during induction, treating the fruit balls with cold plasmas generated by atmospheric pressure dielectric barrier discharge every 5-6 hours. By adoption of fresh fruit balls as explants for tissue culture propagation, low propagation cost, simple propagation process, high speed in whole tissue culture propagation and short culture period are realized, and large-scale development of seedlings can be realized quickly.

The invention discloses a method for promoting cuttage survival of ficus tikoua bureau. The method includes steps: soaking branches of ficus tikoua bureau in ABT2 solution, then subjecting cuttings officus tikoua bureau to cuttage in a medium, and thoroughly watering. The method has advantages of high cuttage survival rate, low propagation cost, high strong seedling rate and effectiveness in promotion of cuttage survival of ficus tikoua bureau and promotion of development of the ficus tikoua bureau industry.