Method for propagating Japan exorista japonica with wax moth

A technology of wax moth and parasitic fly chasing, which is applied in animal husbandry and other fields, can solve the problem of low host parasitism rate, and achieve the effects of high reproductive efficiency, low feeding cost and short feeding cycle

Active Publication Date: 2020-06-12
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The activity ability of the larvae of the greater wax moth is strong and the habit of spinning silk. In the experiment, it was found that the larvae of the greater wax moth had found a hiding place and spun silk to wrap themselves before the parasitic fly laid eggs, resulting in a low host parasitism rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Collection of Provenance

[0021] Collect the mature larvae of the large bag moth, put them into the fly collection cage, collect the Japanese chasing fly adults and move them into the new fly collection cage as a provenance, and remove the weak and deformed parasite flies. 50 Japanese chasing flies were moved into each cage, and the ratio of male to female was 2-3:2. Put into 20% honey water in the cage, supplement nutrition for the adult.

[0022] (2) Inoculation

[0023] After the parasites were moved into the breeding cage for 3 days, 50 mature larvae of the wax moth which were treated with 75% alcohol + 60°C hot water were placed in a petri dish, put into the breeding cage, and parasite eggs were collected every 2 hours.

[0024] (3) Collection of parasite eggs

[0025] Take out the petri dish, observe it under a dissecting microscope, move the larvae of the parasitic fly, Melonella mellonella, which have laid eggs, into a disposable transparent plastic cup,...

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PUM

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Abstract

The invention relates to a method for propagating Japan exorista japonica with wax moth. The method comprises the steps of putting 60 Japan exorista japonica pupas which are pupated in the same day ina raising cage; placing wax moth larvae treated with 75% alcohol and hot water of 60 DEG C with a culture dish, and placing the culture dish in the raising cage; taking out the culture dish each 2h,moving the wax moth larvae on which the exorista japonica lays eggs, to a disposable plastic cup, wherein 50 exorista japonica pupae are placed in each cup; after 15-20d, taking out the exorista japonica from the disposable transparent plastic cup, and integrally placing the exorista japonica in a new disposable transparent plastic cup, wherein 60 exorista japonica pupae are placed in each disposable transparent plastic cup; and after the exorista japonica are subjected to emergence, moving the disposable transparent plastic cup into a new exorista japonica collecting cage, and starting new round of raising and propagating. The host wax moth larvae used by the method are easy to obtain, simple to operate, and low in cost for raising Japan exorista japonica.

Description

technical field [0001] The invention belongs to the technical field of biological control and relates to a breeding method for natural enemy insects, in particular to a method for breeding Japanese parasites by using the greater wax moth. Background technique [0002] Exorista japonica is a large egg-type parasitic fly. The female fly lays eggs that have not yet completed embryonic development on the surface of the host body. After hatching, the larvae drill into the body cavity of the host larvae, live a vegetative parasitic life, and finally in the host pupal stage The stage drills out the host, and pupates next to the pupa of the killed host, thereby playing the role of biological control. The parasitic fly has a wide range of hosts. More than 30 kinds of pests such as silkworm, pine caterpillar, American white moth, armyworm, and Spodoptera litura are its natural hosts, and the parasitic rate can reach as high as more than 90%. Therefore, the parasitic fly has broad app...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 蒋学建黄华艳罗辑常明山邹东霞
Owner GUANGXI FORESTRY RES INST
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