A kind of method that utilizes greater wax moth to breed Japanese parasite fly

Abstract

The invention relates to a method for breeding japonica by using S. mellonella moth to reproduce 60 japonica pupae that pupated on the same day into a breeding cage. Melonella mellonella larvae treated with 75% alcohol + 60°C hot water were placed in a petri dish and placed in a breeding cage. The petri dish was taken out every 2 hours, and the larvae of the parasitic flies had been transferred to disposable plastic cups, 50 per cup. After 15‑20 days, take out the parasite pupae from the disposable transparent plastic cup and place them in a new disposable transparent plastic cup, and the number of parasite pupae in each disposable transparent plastic cup is 60. When any parasitic fly emerges, the disposable transparent plastic cup is moved into a new fly collection cage to start a new round of breeding. The method uses the host greater mellonella larvae which are easier to obtain, the operation is simple, and the cost of rearing the japonica fly is low.

Description

technical field

[0001] The invention belongs to the technical field of biological control and relates to a breeding method for natural enemy insects, in particular to a method for breeding Japanese parasites by using the greater wax moth. Background technique

[0002] Exorista japonica is a large egg-type parasitic fly. The female fly lays eggs that have not yet completed embryonic development on the surface of the host body. After hatching, the larvae drill into the body cavity of the host larvae, live a vegetative parasitic life, and finally in the host pupal stage The stage drills out the host, and pupates next to the pupa of the killed host, thereby playing the role of biological control. The parasitic fly has a wide range of hosts. More than 30 kinds of pests such as silkworm, pine caterpillar, American white moth, armyworm, and Spodoptera litura are its natural hosts, and the parasitic rate can reach as high as more than 90%. Therefore, the parasitic fly has broad app...

Examples

Embodiment 1

[0020] (1) Collection of Provenance

[0021] Collect the mature larvae of the large bag moth, put them into the fly collection cage, collect the Japanese chasing fly adults and move them into the new fly collection cage as a provenance, and remove the weak and deformed parasite flies. 50 Japanese chasing flies were moved into each cage, and the ratio of male to female was 2-3:2. Put into 20% honey water in the cage, supplement nutrition for the adult.

[0022] (2) Inoculation

[0023] After the parasites were moved into the breeding cage for 3 days, 50 mature larvae of the wax moth which were treated with 75% alcohol + 60°C hot water were placed in a petri dish, put into the breeding cage, and parasite eggs were collected every 2 hours.

[0024] (3) Collection of parasite eggs

[0025] Take out the petri dish, observe it under a dissecting microscope, move the larvae of the parasitic fly, Melonella mellonella, which have laid eggs, into a disposable transparent plastic cup,...

technical field

[0001] The invention belongs to the technical field of biological control and relates to a breeding method for natural enemy insects, in particular to a method for breeding Japanese parasites by using the greater wax moth. Background technique

[0002] Exorista japonica is a large egg-type parasitic fly. The female fly lays eggs that have not yet completed embryonic development on the surface of the host body. After hatching, the larvae drill into the body cavity of the host larvae, live a vegetative parasitic life, and finally in the host pupal stage The stage drills out the host, and pupates next to the pupa of the killed host, thereby playing the role of biological control. The parasitic fly has a wide range of hosts. More than 30 kinds of pests such as silkworm, pine caterpillar, American white moth, armyworm, and Spodoptera litura are its natural hosts, and the parasitic rate can reach as high as more than 90%. Therefore, the parasitic fly has broad app...

Examples

Embodiment 1

[0020] (1) Collection of Provenance

[0021] Collect the mature larvae of the large bag moth, put them into the fly collection cage, collect the Japanese chasing fly adults and move them into the new fly collection cage as a provenance, and remove the weak and deformed parasite flies. 50 Japanese chasing flies were moved into each cage, and the ratio of male to female was 2-3:2. Put into 20% honey water in the cage, supplement nutrition for the adult.

[0022] (2) Inoculation

[0023] After the parasites were moved into the breeding cage for 3 days, 50 mature larvae of the wax moth which were treated with 75% alcohol + 60°C hot water were placed in a petri dish, put into the breeding cage, and parasite eggs were collected every 2 hours.

[0024] (3) Collection of parasite eggs

[0025] Take out the petri dish, observe it under a dissecting microscope, move the larvae of the parasitic fly, Melonella mellonella, which have laid eggs, into a disposable transparent plastic cup,...

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