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Cedar pollen tissue culture propagation method

A tissue culture and Chinese fir technology, applied in the field of Chinese fir planting, can solve problems such as not achieving good results, and achieve the effects of short cultivation period, large-scale development of seedlings, and simple reproduction process.

Inactive Publication Date: 2017-05-31
阜阳市颍泉区玉寿种植专业合作社
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

[0002] Chinese fir is a unique timber tree species in my country and one of the important tree species for afforestation in southern provinces and regions. Chinese fir grows rapidly, has a straight and round stem shape, light and tough material, beautiful texture, easy processing, fragrant wood, strong, non-deformed, and resistant Ant-boring is wear-resistant and corrosion-resistant, and is widely used in house construction, furniture, shipbuilding, and making utensils. With the deep processing and utilization of Chinese fir, Chinese fir has become more and more popular and loved by the public, and has a bright future. did not achieve good results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1, a kind of Chinese fir pollen tissue culture propagation method, comprises the following steps:

[0009] After the Chinese fir blooms, pollinate manually. After the Chinese fir bears fruit balls, pick the fruit balls manually, dip the fruit balls in a silver iodide aqueous solution with a mass concentration of 3%, and treat them under ultrasonic waves with a power of 180W for 15 seconds; The fruit balls after inoculation were induced to take root and shoot in the medium composed of MS medium+ε-polylysine 1mg / L+sodium pyrophosphate 0.2mg / L+divalent calcium ion 1.8mg / L. During the induction period, every The fruit balls were treated with cold plasma generated by atmospheric pressure dielectric barrier discharge for 5 hours, and the treatment time was 10 seconds each time. The power supply of the dielectric barrier discharge was a high-frequency high-voltage power supply with a frequency range of 3kHz and a voltage range of 2kV.

Embodiment 2

[0010] Embodiment 2, a kind of Chinese fir pollen tissue culture propagation method, comprises the following steps:

[0011] After the Chinese fir blooms, pollinate manually. After the Chinese fir bears fruit balls, manually pick the fruit balls, dip the fruit balls in a silver iodide aqueous solution with a mass concentration of 4%, and treat them under ultrasonic waves with a power of 200W for 18 seconds; The fruit balls after inoculation were induced to take root and shoot in the medium composed of MS medium+ε-polylysine 1.2mg / L+sodium pyrophosphate 0.3mg / L+divalent calcium ion 2mg / L. During the induction period, every The fruit balls were treated with cold plasma generated by atmospheric pressure dielectric barrier discharge for 6 hours, and the treatment time was 15 seconds each time. The power supply of the dielectric barrier discharge was a high frequency and high voltage power supply with a frequency range of 5kHz and a voltage range of 3kV.

[0012] Below in conjuncti...

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Abstract

The invention relates to the technical field cedar planting, in particular to a cedar pollen tissue culture propagation method. The method includes steps: impregnating fruit balls into silver iodide aqueous solution to realize ultrasonic sterilization; performing inoculation of the sterilized fruit balls in a culture medium to realize root and germination induction, wherein the culture medium is formed by an MS culture medium, 1-1.2mg / L epsilon-polylysine, 0.2-0.3mg / L sodium pyrophosphate and 1.8-2mg / L divalent calcium ions; during induction, treating the fruit balls with cold plasmas generated by atmospheric pressure dielectric barrier discharge every 5-6 hours. By adoption of fresh fruit balls as explants for tissue culture propagation, low propagation cost, simple propagation process, high speed in whole tissue culture propagation and short culture period are realized, and large-scale development of seedlings can be realized quickly.

Description

technical field [0001] The invention relates to the technical field of Chinese fir planting, in particular to a method for tissue culture and propagation of Chinese fir pollen. Background technique [0002] Chinese fir is a unique timber tree species in my country and one of the important tree species for afforestation in southern provinces and regions. Chinese fir grows rapidly, has a straight and round stem shape, light and tough material, beautiful texture, easy processing, fragrant wood, strong, non-deformed, and resistant Ant-boring is wear-resistant and corrosion-resistant, and is widely used in house construction, furniture, shipbuilding, and making utensils. With the deep processing and utilization of Chinese fir, Chinese fir has become more and more popular and loved by the public, and has a bright future. Did not achieve better results. Contents of the invention [0003] The invention provides a fir pollen tissue culture propagation method. [0004] The technical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02
CPCA01H4/001A01H1/02A01H4/008
Inventor 张玉李玉龙
Owner 阜阳市颍泉区玉寿种植专业合作社
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