Wild cabbage BoMS1 gene and application thereof to preparation of sterile materials

Abstract

The invention belongs to the field of biotechnology breeding, and specifically relates to a wild cabbage BoMS1 gene and an application thereof to preparation of sterile materials. The invention provides a wild cabbage BoMS1 gene namely a key gene for controlling wild cabbage males to be sterile, the expression of the wild cabbage BoMS1 gene is lowered through an RNAi technique, and further a genetically modified male sterile plant system is prepared, so that the problem that heredity weakness risk exists because single ogura CMS single sterile resources are massively depended when wild cabbagefirst filial generation variety breeding is performed in a male sterile way is solved. The expression of the wild cabbage BoMS1 gene and the expression of a wild cabbage SRK gene are lowered throughthe RNAi technique, and the genetically modified male sterile system which is compatible with blossoming hybridization of a maintenance line is prepared. Through the adoption of sterile strains prepared according to the method, the problem that the propagation of the maintenance line needs artificial bud pollination is solved, and the propagation cost of wild cabbage male sterile systems is lowered; a non-genetically modified male sterile system which is compatible with blossoming hybridization of the maintenance line is further prepared through simultaneous mutation of the wild cabbage BoMS1gene and the wild cabbage SRK gene.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to the cabbage BoMS1 gene and its application in creating sterile materials. Background technique [0002] Cabbage is one of the most widely planted cruciferous vegetables in the world, and it is also one of the important autumn and winter leafy vegetables in my country. The annual planting area of ​​cabbage in China is about 12 million mu. The first-generation hybrid varieties can greatly increase the yield and stress resistance of cabbage, and the popularization and utilization of the first-generation hybrid varieties is an important way to improve the production efficiency of cabbage. For a long time, the selection of the first-generation hybrid varieties of cabbage was mainly achieved by using the self-incompatibility approach, that is, using two different S-haplotype self-incompatibility lines to cross through bee pollination during flowering, from the two Th...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above-mentioned problem of genetic vulnerability risk that relies heavily on a single oguraCMS single sterility resource when using the male sterility approach to select the first-generation cabbage hybrid variety, the present invention provides a key gene BoMS1 for controlling male sterility in cabbage. By down-regulating the expression of BoMS1 or mutating the BoMS1 gene, corresponding transgenic male sterile lines and non-transgenic male sterile lines can be obtained

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