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Rapid breeding method for tissue culture of menispermum dauricum

A tissue culture and bat kudzu technology, applied in the field of plants, can solve problems such as unresearched reproduction methods, and achieve the effects of short cycle, large-scale planting, and large yield.

Inactive Publication Date: 2015-01-21
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mainly adopt sowing and branch propagation, and the propagation method of tissue culture has not been studied yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the seeds of Pueraria japonica, clean them, soak them in 100mg / L gibberellin solution for 16 hours, treat them with 70% alcohol for 50 seconds on the ultra-clean workbench, treat them with 15% sodium hypochlorite for 15 minutes, wash them with running water for 5 times, and insert them into MS medium Cultivate sterile seedlings in medium, add sucrose 30g / L, agar 7g / L, pH5.8, light 3000lx, temperature 22±2°C, remove the radicle of the obtained sterile seedlings, inoculate in MCM+1.5mg / LZT+0.15mg Proliferation culture of clustered buds in / LKT medium, supplemented with 30g / L sucrose, 7g / L agar, pH5.8, light 3500lx, temperature 23±2°C, the proliferated clustered buds were inserted into MCM+1mg / L6-BA +0.025mg / LIAA+3mg / LKT medium for strong seedlings, add sucrose 60g / L, agar 7g / L, pH5.8, light 4500lx, temperature 25±2℃, take out the sprouts about 3cm long, wash Remove the root medium, plant in sterilized humus soil, set up a small shade shed, water 0.6mg / LABT rooting ag...

Embodiment 2

[0011] Take the seeds of Pueraria japonica, clean them, soak them in 100mg / L gibberellin solution for 16 hours, treat them with 70% alcohol for 50 seconds on the ultra-clean workbench, treat them with 15% sodium hypochlorite for 15 minutes, wash them with running water for 5 times, and insert them into MS medium Cultivate sterile seedlings in medium, add sucrose 30g / L, agar 7g / L, pH5.8, light 3000lx, temperature 22±2°C, remove the radicle of the obtained sterile seedlings, inoculate in MCM+1.5mg / LZT+0.15mg Proliferation culture of clustered buds in / LKT medium, supplemented with 30g / L sucrose, 7g / L agar, pH5.8, light 3500lx, temperature 23±2°C, the proliferated clustered buds were inserted into MCM+2mg / L6-BA +0.5mg / LIAA+4mg / LKT medium for strong seedlings, add sucrose 60g / L, agar 7g / L, pH5.8, light 4500lx, temperature 25±2℃, take out the sprouts about 3cm long, wash Remove the root medium, plant in sterilized humus soil, set up a small shade shed, water 0.6mg / LABT rooting agen...

Embodiment 3

[0013] Take the seeds of Pueraria japonica, clean them, soak them in 100mg / L gibberellin solution for 16 hours, treat them with 70% alcohol for 50 seconds on the ultra-clean workbench, treat them with 15% sodium hypochlorite for 15 minutes, wash them with running water for 5 times, and insert them into MS medium Cultivate sterile seedlings in medium, add sucrose 30g / L, agar 7g / L, pH5.8, light 3000lx, temperature 22±2°C, remove the radicle of the obtained sterile seedlings, inoculate in MCM+1.5mg / LZT+0.15mg Proliferation culture of clustered buds in / LKT medium, supplemented with 30g / L sucrose, 7g / L agar, pH5.8, light 3500lx, temperature 23±2°C, the proliferated clustered buds were inserted into MCM+2mg / L6-BA +0.025mg / LIAA+4mg / LKT medium for strong seedlings, add 60g / L sucrose, 7g / L agar, pH5.8, light 4500lx, temperature 25±2℃, take out the sprouts about 3cm long, wash Remove the root medium, plant in sterilized humus soil, set up a small shade shed, water 0.6mg / LABT rooting ag...

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PUM

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Abstract

The invention relates to a rapid breeding method for the tissue culture of menispermum dauricum. The method comprises the steps of obtaining aseptic seedlings of the menispermum dauricum so as to obtain cluster buds, carrying out multiplication on the cluster buds to obtain seedlings, strengthening the seedlings, carrying out field root induction, and the like. According to the method, the aseptic seedlings are obtained by adopting methods of sterile sowing and the tissue culture, and the steps of strengthening the seedlings, carrying out rooting culture and transplanting are carried out, so that the method is low in breeding cost and short in cultivation period; furthermore, the method is simple and easy to operate and convenient to popularize, and the technical support is provided for development and utilization as well as rapid propagation of high-quality varieties.

Description

technical field [0001] The invention relates to a method for rapid propagation of kudzu tissue culture and belongs to the technical field of plants. Background technique [0002] Bat kudzu, Fangjiaceae, Menispermum dauricum DC , also known as Shandougen, Huangtiaoxiang, Shandouyangen, Nienba. Perennial herbaceous flowers, deciduous vines, green branchlets, alternate leaves, reniform or oval, 5 to 12 cm long, entire or 3 to 7 lobed, with petioles peltate. Flowers unisexual, short panicles axillary, small flowers, light green, flowering period from May to June. The drupe is nearly spherical, purple-black, and the fruit period is from July to September. Cold-resistant, it mostly grows on mountain forest margins, shrub valleys or winding rocks at an altitude of 200 to 1500. Distributed in Northeast China, North China and East China; also in North Korea, Japan, and Siberia of the Soviet Union. The roots and stems contain a variety of alkaloids such as sorghine and tetrandrin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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