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Efficient breeding method of purple potato tissue culture seedlings

A technology of purple potato and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of slow propagation speed of miniature potato cuttings, low cultivation efficiency of purple potato tissue culture seedlings, and the incidence of virus in tissue culture seedlings Advanced problems, to achieve the effect of increasing the propagation speed of test-tube plantlets, reducing the cost of reproduction, and improving economic benefits

Active Publication Date: 2015-12-30
NINGBO ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the technical problems of low cultivation efficiency of purple potato tissue culture seedlings, high incidence of virus in tissue culture seedlings, and slow propagation speed of micropotato cuttings in the prior art, the present invention provides a cultivation method with high cultivation efficiency, low incidence rate, good cutting effect and cultivation method. High-efficiency breeding method of low-cost purple potato tissue culture seedlings

Method used

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  • Efficient breeding method of purple potato tissue culture seedlings

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Effect test

Embodiment 1

[0044] 1) Ultra-low temperature treatment of purple potato tubers:

[0045]Take purple potato tubers with good appearance, healthy and disease-free, and exercise them at a low temperature of 0-2°C for 7 days, then perform ultra-low temperature treatment at -185°C for 0.5 hours, and then thaw and restore the purple potato tubers in the later stage.

[0046] 2) Induction culture of seed potato root tip and shoot tip:

[0047] Take the purple potato tubers with excellent appearance after recovery and culture and bury them in sterilized sand for cultivation. The sterilized sand environment is 15-17°C in temperature and 61-65% in humidity. After 5-8 weeks, it grows roots and new shoots. Induction culture of root tip and shoot tip;

[0048] Specifically include the following steps:

[0049] (1) Configure shoot tip induction medium: MS+0.09mg / LNAA+3.5% white sugar+70% soybean cake powder, pH5.8;

[0050] Cut off the terminal buds of 0.5-0.8cm new shoots, wash them with sterile wat...

Embodiment 2

[0070] 1) Ultra-low temperature treatment of purple potato tubers:

[0071] Take purple potato tubers with good appearance, healthy and disease-free, and exercise them at a low temperature of 0-2°C for 7 days, then perform ultra-low temperature treatment at -175°C for 0.5 hours, and then thaw and resume cultivation of purple potato tubers;

[0072] 2) Induction culture of seed potato root tip and shoot tip:

[0073] Take the purple potato tubers with excellent appearance after recovery and bury them in sterilized sand for cultivation. The temperature of sterilized sand is 15-17°C and the humidity is 61-65%. After 5-8 weeks, they will grow roots and new shoots. , inducing culture of root tip and shoot tip;

[0074] Specifically include the following steps:

[0075] Configure shoot tip induction medium: MS + 0.05mg / LNAA + 3.5% white sugar + 60% soybean cake powder, pH5.8; cut off the terminal buds of 0.5-0.8cm new shoots, first wash them with sterile water for 3-5 times, and t...

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Abstract

The invention provides an efficient breeding method of purple potato tissue culture seedlings. The efficient breeding method of the purple potato tissue culture seedlings comprises the following steps of treating purple potato tubers at ultralow temperature; performing induced culture on root tips and stem tips of seed potatoes; performing rooting culture on strong seedlings, and conducting virus detection on test-tube plantlets; performing induced culture and virus detection on miniature potatoes; performing transplanting and cuttage grafting breeding on the miniature potatoes. According to the efficient breeding method of the purple potato tissue culture seedlings, provided by the invention, miniature potato seedlings are bred by adopting virus eradication of stem tips and root tips; the culture cycle of the root tips is different from that of the stem tips; the root tips and the stem tips are jointly cultured, so that the time cost can be effectively reduced, seed potato resources are fully and efficiently utilized, and further the reproduction cost of the tissue culture seedlings is reduced; besides, the test-tube plantlets cultured by using the root tips are high in reproduction speed, so that the working efficiency is improved, and the production cost is reduced.

Description

technical field [0001] The invention relates to plant tissue culture rapid propagation technology, in particular to a high-efficiency propagation method of purple potato tissue culture seedlings. Background technique [0002] Purple potato is an annual crop of Solanaceae, native to Australia. Purple potatoes, also known as black potatoes, are oblong in shape and have smaller bud eyes. The peel is dark purple, jet black and shiny, rich in luster. The flesh is dark purple, good-looking and attractive in color. The pulp starch content is as high as 13-15%, and the taste is good and the quality is excellent. According to the determination: every 100 grams of black potatoes contains 2.3 grams of protein, 0.1 grams of fat, 16.5 grams of carbohydrates, 11 milligrams of calcium, 1.2 milligrams of iron, 64 milligrams of phosphorus, 342 milligrams of potassium, 22.9 milligrams of magnesium, 0.01 milligrams of carotene, thiamine 0.1 mg riboflavin, 0.03 mg riboflavin, 16 mg niacin. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王芳黄坚刘健严成其陆旭峰
Owner NINGBO ACAD OF AGRI SCI
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