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Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts

A technology for S. tumefaciens and barley worm is applied in the field of using barley worm as an intermediate host to breed S. tumefaciens, which can solve the problems of increased cost, numerous equipment, cumbersome steps and the like, and achieves simplified steps, reduced reproduction cost, and overcomes time. The effect of restriction

Inactive Publication Date: 2013-04-03
北京市西山试验林场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, S. guanidei must first be inoculated on an induced host to induce oviposition, and then it can be bred on an alternative host, which leads to cumbersome steps and a large number of equipment used, which greatly increases the cost.

Method used

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  • Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts
  • Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts
  • Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts

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Experimental program
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Effect test

Embodiment 1

[0036] 1. Infrastructure: numerous beehives, breeding racks.

[0037] 2. Instruments and equipment: heating and cooling air conditioners, humidifiers, air exchangers, electric heaters, temperature and humidity controllers.

[0038] 3. Main appliances and consumables: finger tubes, tampons, writing brushes, feeding baskets.

[0039] 4. Artificial bee breeding host

[0040] Host species: barley worms.

[0041] Host collection: Collect fresh barley worm pupae as intermediate hosts.

[0042] Host storage: 1 month at 4°C.

[0043] 5. Breeding bees

[0044] 5.1 species of bees get:

[0045] (1) Collect beetle parasites parasitized by Scleroderma guanii by cutting wood in the field, and put them in artificial intelligence culture (T=26±1°C, RH=60~70%, D:L=24) until day one. Scleroderma guanidei on the bovine worm develops into adults.

[0046] (2) Introduce strong individual female bees from areas where Scleroderma guanii is widely used.

[0047] Breeding bee selection: choos...

Embodiment 2

[0072] The source of the tested insects: Scleroderma guanidei pupae were collected from cypress trees parasitized by the longhorn beetle in Xishan Forest Farm, and they were bred and preserved in a constant temperature room (26±1°C, RH70%~80%) with the larvae of the long beetle 3 generations; intermediate hosts: corn borer and greater wax moth were reared with artificial feed in the laboratory; yellow powder beetles and barley worms were purchased from the market and fed with wheat bran in a constant temperature room.

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Abstract

The invention relates to a method for breeding Scleroderma guani Xiao et Wu in a non-induced way. The method comprises the steps of female warp obtaining, storage, multiplying, rejuvenation, propagation expanding, quality inspection, packaging, storage, transportation and the like. The method for breeding the Scleroderma guani Xiao et Wu in a non-induced way, provided by the invention, is mainly used for multiplying the Scleroderma guani Xiao et Wu by using barley pests as alternative hosts.

Description

technical field [0001] The invention relates to a method for breeding Sclerodon guanii by parasitism, in particular to a method for breeding Sclerodon guanii by using barley worms as intermediate hosts. Background technique [0002] Tree borer pests such as longhorn beetle are very difficult to control because of their hidden hazards and other characteristics. They are one of the destructive pests of forest trees and seriously threaten my country's forestry construction and ecological security. For example, pine wood nematode disease is transmitted by the pine wood nematode, and once the pine tree is infected, it cannot be cured. In my country, hundreds of millions of pine trees have died due to the infection of pine wood nematode disease, and the economic loss has exceeded 100 billion yuan; Pests have caused the death of nearly ten million Chinese pine trees; the planting area of ​​poplar trees in my country has reached 100 million mu, which is more than the total area of ​​...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 梁洪柱陈倩田会鹏
Owner 北京市西山试验林场
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