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A method for the propagation and rejuvenation of scleroderma guani Xiao et Wu with Chinese chestnut weevils as intermediate hosts and the use thereof

A technology for a swollen leg wasp and a host, which is applied in the field of insects, can solve the problems of decreased parasitic ability and population degradation of the tube-type swollen leg wasp, and achieves the effects of simple equipment, reduced reproduction cost, and broad industrialization prospects.

Inactive Publication Date: 2017-02-15
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the large-scale artificial breeding of Scleroderma tubelis indoors using the chrysalis chrysalis pupae is relatively mature, but after several generations of artificial breeding, the population of Scleroderma tuberosi will degenerate and the parasitic ability will decline. Rejuvenation of tube swollen bees is required
[0004] So far, there is no report on the method and application of chestnut weevil to multiply and multiply Scleroderma burdii as an intermediate host

Method used

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  • A method for the propagation and rejuvenation of scleroderma guani Xiao et Wu with Chinese chestnut weevils as intermediate hosts and the use thereof
  • A method for the propagation and rejuvenation of scleroderma guani Xiao et Wu with Chinese chestnut weevils as intermediate hosts and the use thereof
  • A method for the propagation and rejuvenation of scleroderma guani Xiao et Wu with Chinese chestnut weevils as intermediate hosts and the use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Instruments and equipment: artificial intelligence incubator.

[0037] 2. Main appliances and consumables: finger tubes, cotton, brushes, trays, tweezers, towels, alcohol.

[0038] 3. Artificial bee breeding host

[0039] Host species: chestnut weevil

[0040] Host collection: collect mature larvae of chestnut weevil as alternative hosts.

[0041] Host storage: store at 10°C for 1 month.

[0042] 1 bee

[0043] 1.1 species of bees get:

[0044] (1) Collect the long beetle worms parasitized by Scleroderma guanii in the field, and place them in the artificial intelligence incubator (26°C, RH 65%, D:L=24), until the tubes on the long beetle worms All Scleroderma sclerodermas develop into adults.

[0045] (2) Purchase healthy individual bees from laboratories or companies that breed Scleroderma guanii.

[0046] Breeding bee selection: choose wingless female bees that are large, strong, lively, and mated.

[0047] Breeding bee propagation: each finger-shaped tube ...

Embodiment 2

[0064] The degenerated Scleroderma guanii was bred for 5 generations with the yellow powder beetle, and the ratio of male to female was selected to be 2:1, and placed in a culture tube. One day after natural mating, one female bee was placed in the same area as a chestnut weevil. In the larvae breeding box, cultured for 2 days in an environment with a temperature of 27°C and a humidity of 70%. Under the microscope, 29 chestnut weevil larvae were seen to lay eggs. After 26 days of culture in a conventional breeding environment, 27 bee cocoons were collected and the cocoons emerged. According to statistics, the average body length of bees is 3.5376±0.3079mm, and the weight is 0.8201±0.1385g, both of which are significantly higher than those of the previous generation.

Embodiment 3

[0066] The degenerated Scleroderma guanii was bred for 4 generations with the yellow powder beetle, and the ratio of male to female was selected to be 3:1, and placed in a culture tube. After natural mating, 3 female bees were placed in the same place as a chestnut weevil. The larvae were cultured in the larva breeding box for 1 day at a temperature of 25°C and a humidity of 55%. Under the microscope, it was seen that the chestnut weevil larvae produced 41 eggs. After 26 days of culture in a conventional culture environment, 41 bee cocoons were collected and the cocoons emerged. According to statistics, the average body length of bees is 3.7012±0.2331mm, and the weight is 0.8137±0.0781g, both of which are significantly higher than those of the previous generation.

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Abstract

The invention relates to a method for the propagation and rejuvenation of scleroderma guani Xiao et Wu with Chinese chestnut weevils as intermediate hosts and the use thereof and belongs to the technical field of insects. Mature larvae of Chinese chestnut weevils, as hosts, and mated scleroderma guani Xiao et Wu females, as breeding bees, are put in a culture container, wherein the ratio of the number of the breeding bees to the number of the hosts is 1-3:1; culture is carried out for 1-2 days with the culture container having a temperature of 25-27 DEG C and a humidity of 55-70%; after the breeding bees oviposit, scleroderma guani Xiao et Wu goes through the larval phase and the pupal phase and grows to adults. The breeding bees can be scleroderma guani Xiao et Wu which has degenerated after breeding for 4-5 generations in other hosts. The use of the method is that the obtained scleroderma guani Xiao et Wu adults can perform parasitic propagation in stem borers and thus kill the stem borers. Chinese chestnut weevil larvae are used as substitute hosts for the propagation of scleroderma guani Xiao et Wu and inducing hosts are not needed, so that the breeding cycle of scleroderma guani Xiao et Wu is greatly shortened, steps are simplified and the breeding cost is reduced; the utilization rate of Chinese chestnut weevil larvae, as substitute hosts, is higher than that of mature larvae of corn borers and pupae of tenebrio molitors.

Description

technical field [0001] The invention belongs to the technical field of insects, and in particular relates to a method for multiplying and rejuvenating Scleroderma cuspidatum using chestnut weevil as an intermediate host and its application. Background technique [0002] Scleroderma guani Xiao et Wu, belonging to Hymenoptera (Hymenoptera), Scleroderma guani superfamily Bethyloidea), Scleroderma guani family (Bethylidae), is a boring pest, especially the larvae and pupae The ectoparasitic natural enemies play an important role in the control of dry borer pests. At present, the application research of Scleroderma guanii has become one of the hotspots in the biological control of dry-boring pests. Has been successful. [0003] At present, the large-scale artificial breeding of Scleroderma tubelis indoors using the chrysalis chrysalis pupae is relatively mature, but after several generations of artificial breeding, the population of Scleroderma tuberosi will degenerate and the ...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01G13/00
CPCA01G13/00A01K67/033
Inventor 秦小萍吴国星吴道慧高熹何霞红李强杨文波张扬
Owner YUNNAN AGRICULTURAL UNIVERSITY
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