In vivo reproduction method of entomopathogenic nematodes

A technology of entomopathogenic nematodes and live bodies, which is applied in the field of in vivo culture of entomopathogenic nematodes, can solve the problems of low yield, high reproduction cost, poor pathogenicity, etc., and achieve the effects of high yield, large volume and reduced cost

Inactive Publication Date: 2012-08-22
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of high reproduction cost, low yield, and poor pathogenicity in the existing living culture methods of entomopathogenic nematodes, and to provide a living reproduction method of entomopathogenic nematodes

Method used

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Examples

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Effect test

specific Embodiment approach 1

[0006] Embodiment 1: This embodiment is a method for in vivo propagation of entomopathogenic nematodes, which is specifically completed by the following steps:

[0007] First, cut two medium-speed qualitative filter papers according to the size of the lid of Petri dish A, and put them in the lid of Petri dish A, and then put 8 to 12 5th instar Spodoptera litura larvae in the middle of the lid of Petri dish A. On the rapid qualitative filter paper, then use 0.4 mL to 0.6 mL of the aqueous suspension of 5500 / mL to 6500 nematodes / mL infestation stage nematodes to infiltrate the medium-speed qualitative filter paper in the lid of the petri dish A, and then put the medium-speed qualitative filter paper in the lid of the petri dish A. The bottom was buckled on the lid of Petri dish A until all 8 to 12 5th instar larvae of Spodoptera litura were dead, then the bottom of Petri dish A was removed, and the lid of Petri dish A was placed in Petri dish B, and then placed in Petri dish B. ...

specific Embodiment approach 2

[0009] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the nematodes in the water suspension of the 5500 / mL to 6500 / mL infestation period nematodes are Heterorhabditis bacteriophora-NJ and Steinernema carpocapsae-All. Others are the same as the first embodiment.

specific Embodiment approach 3

[0010] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the diameter of the culture dish A is 60 mm, and the diameter of the culture dish B is 90 mm. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses an in vivo reproduction method of entomopathogenic nematodes, relating to an in vivo culture method of entomopathogenic nematodes and aiming to solve the problems of low yield in applying the tenebrio molitors to breed the nematodes. The in vivo reproduction method comprises the following steps of: firstly, cutting two pieces of medium speed qualitative filter paper and filling in a cover of a culture dish A; then placing prodenia litura larvae; then soaking the medium speed qualitative filter paper by using water suspension infective juveniles; covering the bottom of the culture dish A to the cover of the culture dish A till all prodenia litura larvae die; removing the bottom of the culture dish A; placing the cover of the culture dish A in a culture dish B; filling sterile water to a gap between the culture dish B and the culture dish A; culturing at a constant temperature till the infective juveniles occur in the sterile water; then collecting the sterile water with the infective juveniles every day; and complementing the sterile water so as to complete the in vivo reproduction of the entomopathogenic nematodes. The in vivo reproduction method of the entomopathogenic nematodes is mainly used for the in vivo reproduction of the entomopathogenic nematodes.

Description

technical field [0001] The invention relates to a method for in vivo culture of entomopathogenic nematodes. Background technique [0002] Entomopathogenic Nematode (EPN) is an important natural enemy of pests, has a wide range of host insects, and is currently an important means of biological control of pests. Because entomopathogenic nematodes can be cultured in large quantities at low cost and high efficiency, its propagation technology is constantly developing, from in vivo culture to in vitro culture, from aseptic culture in in vitro culture to single bacteria culture, single bacteria The culture method is further divided into solid-phase culture method and liquid-phase culture method, which makes it move towards the scale of factory production. However, in laboratory research, the White trap live reproduction method has been used, that is, the hydrotropy of nematodes is used. When the nematodes exhaust the organic matter in the host body, the infesting nematodes (IJs) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 李春杰司兆胜
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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