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Yellow-head catfish meat semen preserving fluid and preparation method thereof

A preservation solution and technology of yellow catfish, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of short survival time of yellow catfish sperm, achieve reduced reproduction costs, simple preparation process, and increase production Effect

Inactive Publication Date: 2014-01-22
南京市水产科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to disclose a kind of yellow catfish semen preservation solution, the collected yellow catfish semen is preserved in the preservation solution, the sperm is in an inactive state, and solves the problem of short survival time of yellow catfish sperm after activation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] According to mass volume fraction, mix 0.5%-1.5% mass volume fraction of trisodium citrate, 1.5%-3.5% glucose, 0.1%-0.3% sodium bicarbonate, 0.02%-0.04% potassium chloride and deionized water , placed on a magnetic stirrer and stirred for 3-15 minutes, and then settled to volume after fully dissolving.

Embodiment 2

[0017] According to mass volume fraction, mix 0.5%-1.5% mass volume fraction of trisodium citrate, 1.5%-3.5% glucose, 0.1%-0.3% sodium bicarbonate, 0.02%-0.04% potassium chloride and deionized water , placed on a magnetic stirrer and stirred for 3-15 minutes, after being fully dissolved, make it into a 10X concentrated solution and store at 4°C. When it is ready to use, take 100ml of the 10X concentrated solution and add deionized water to make it up to 1000ml.

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PUM

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Abstract

The invention discloses yellow-head catfish meat semen preserving fluid and a preparation method thereof. The yellow-head catfish meat semen preserving fluid is characterized by being prepared through the following steps: mixing 0.5-1.5% of trisodium citrate, 1.5-3.5% of glucose, 0.1-0.3% of sodium bicarbonate, 0.02-0.04% of potassium chloride and deionized water in percentage by mass volume, putting the mixture into a magnetic stirrer, stirring for 3-15 minutes, and determining the volume after sufficiently dissolving. Tests show that if yellow-head catfish meat semen is preserved in the preserving fluid for 8 hours at normal temperature, 100% of sperms have the activity after being activated, and the fertility rate is 98% when the sperms are used for fertilization; if the semen is preserved for 14 days at the refrigeration condition of 4 DEG C, 100% of the sperms have the activity, and the fertility rate is 95% when the sperms are used for fertilization.

Description

technical field [0001] The invention relates to the technical field of aquatic animal semen preservation, in particular to a yellow catfish semen preservation solution and a preparation method thereof. Background technique [0002] Pelteobagrus fulvidraco (Pelteobagrus fulvidraco) is a small freshwater economic fish that is distributed in all major water systems in my country, especially in the Yangtze River. Widely distributed in downstream lakes, ponds and streams. Pelter catfish is rich in amino acids, tender meat, delicious taste, high nutritional value, no intermuscular thorns, and has nourishing effect and medicinal value. It has been widely recognized by people, and its value has doubled. In the next few years, the breeding area of ​​yellow catfish will continue to expand. [0003] The key to the large-scale cultivation of yellow catfish is to solve the problem of the supply of yellow catfish fry. The existing methods of artificial reproduction of yellow catfish are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 刘炜茆健强周国勤唐忠林孙浩波陆健王颖
Owner 南京市水产科学研究所
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