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Tissue culture rapid propagation method for excellent sequoia sempervirens seedlings

A tissue culture rapid propagation, excellent technology, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of large influence of external environment, low quality of seedling emergence, long breeding cycle, etc., and achieve complete disinfection and killing. , the effect of increasing the reproduction coefficient and increasing the germination rate

Active Publication Date: 2018-02-06
贵州省林业学校 +1
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Problems solved by technology

[0004] At present, the traditional propagation methods of Sequoia chinensis are mainly based on traditional sowing, cutting and branching methods; however, the traditional propagation methods are costly, time-consuming and labor-intensive, have a long reproduction cycle, low reproduction coefficient, and are greatly affected by the external environment. , and the emergence quality is not high, the height is inconsistent, and the differentiation phenomenon is serious

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  • Tissue culture rapid propagation method for excellent sequoia sempervirens seedlings

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Embodiment Construction

[0026] The present invention will be described in detail below in conjunction with embodiments:

[0027] Preparation example one of propiconazole / cobalt chloride complex

[0028] Take propiconazole and cobalt chloride hexahydrate in a ratio of 2.2:1, and then dissolve them in 3 times the mass of absolute ethanol, and drop the cobalt chloride hexahydrate solution at a reflux temperature of 80°C. Add propiconazole solution, continue to reflux and stir for 2h, cool, let stand overnight, remove the filtrate by suction filtration, wash the precipitate with sterile water 4 to 5 times, and then at 55 ~ 60 ℃, 0.09MPa ~ 0.1MPa vacuum conditions The drying time is 4h, and a blue solid is obtained, which is the propiconazole / cobalt chloride complex.

[0029] Example 2 of Tissue Culture and Rapid Propagation of Superior Sequoia Seedlings

[0030] Select sturdy and strong clones of the Sequoia cedar in the current year, and cut 2 to 3 cm long budded stems as explants; rinse the selected budded st...

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Abstract

The invention discloses a tissue culture rapid propagation method for excellent sequoia sempervirens seedlings. The method comprises the following steps: constructing explants, disinfecting the explants, performing differential induction, performing enrichment culture, performing ex vitro rooting, transplanting and cultivating. An excellent clone branch of robust annual sequoia sempervirens is selected and is cut into 2-3cm long stems with buds as the explants for tissue culture rapid propagation. The tissue culture rapid propagation method for excellent sequoia sempervirens seedlings disclosed by the invention is slightly influenced by the external environment, is capable of greatly reducing the propagation cost, shortening the propagation period, increasing the propagation coefficient ofthe seedlings and acquiring the seedlings in highly consistent quality, is convenient for large-scale factory cultivation and has important significance in the aspects of seedling propagation and value utilization of the excellent sequoia sempervirens clone in the future.

Description

Technical field [0001] The invention relates to the technical field of tissue culture and rapid propagation of forest trees, in particular to a method for tissue culture and rapid propagation of excellent seedlings of Sequoia cedar. Background technique [0002] Sequoia sequoia (scientific name: Sequoiasempervirens) is an evergreen tree of the genus Sequoia of the Chinese fir family. It is native to the coast of California, USA. It is introduced and cultivated in Shanghai, Nanjing, Hangzhou, and Guizhou, China. Extra-large tree, the origin is up to 110 meters, the diameter at breast height is 8 meters, evergreen; the leaves have squamous and linear leaves, and the cones are monoecious; the male cones are solitary, with many stamens and female cones. Single branch top with many scaly leaves underneath; 15-20 pearl scales; bracts connate with pearl scales; cones mature in the same year, ovoid, and pearl scales develop into seed scales, woody, shield-shaped when mature; seeds 2 -5 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/06A01G31/02C05G3/00A01N43/653A01N55/02A01N43/90A01N37/10A01P21/00A01P1/00C09D5/02
CPCA01G7/06A01G31/02A01H4/001A01H4/008A01N37/10A01N43/653A01N43/90A01N55/02C05F11/00C05G3/00C09D5/024Y02P60/21
Inventor 田怀侯娜张晓敏刘小红
Owner 贵州省林业学校
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