Method for preventing and controlling hyphantria cunea by using trichomalopsis genalis

A technology of American white moth and golden bee, which is applied in the monitoring of invasive species, climate sustainability, animal husbandry, etc., can solve the problems of unstable number of breeding bees, low success rate of replacing host bees, etc., to reduce artificial breeding Cost, improvement of labor productivity and equipment efficiency, cost saving effect

Active Publication Date: 2021-08-31
SHENYANG AGRI UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to make up for the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for preventing and treating the white moth of the American bollworm by using alternative hosts, the chrysalis chrysalis, to reproduce the cotton bollworm A. The problem of low rate and unstable number of breeding bees, and increase the similarity between the offspring of the cotton bollworm golden wasp obtained by breeding and its natural breeding offspring, so as to ensure the parasitic ability of the cotton bollworm golden wasp to the American white moth. Enrich the dominant parasitoid species of American white moth control, and add new means and new methods for the biological control of American white moth

Method used

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  • Method for preventing and controlling hyphantria cunea by using trichomalopsis genalis
  • Method for preventing and controlling hyphantria cunea by using trichomalopsis genalis
  • Method for preventing and controlling hyphantria cunea by using trichomalopsis genalis

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Effect test

Embodiment 1

[0029] The influence of the breeding method of embodiment 1 different yellow powder beetle pupae on the effect of receiving bees

[0030] Cultivation method of the chrysalis chrysalis Ⅰ: the method of breeding the cotton bollworm golden beetle through gradient cooling of the chrysanthemum chrysalis pupae. It is placed at a temperature of 22-28° C., a relative humidity of 50-70%, and a photoperiod of L:D=16h:8h; it is subcultured in a feeding box with feed such as wheat bran, vegetables and fruits. In the pupation stage, transfer the above-mentioned yellow powdered beetle pupae from 22-28°C into an insect culture box with ventilation holes, and then carry out gradient cooling treatment; the gradient cooling treatment is: placed in a 16°C constant temperature box for 2 hours , and then placed in a 10°C incubator for 2 hours to obtain a pupae with yellow powder for bee inoculation, and place it in a 4°C incubator to protect from light. It can be stored for 1-20 days, so that all ...

Embodiment 2

[0038] Example 2 Comparison of Effects of Different Alternative Hosts for Breeding the Cotton Bollworm Golden Bumblebee

[0039] 1. Host Screening Test

[0040](1) Place the white moth pupae parasitized by the cotton bollworm golden beetle collected in the field at a temperature of 22-28°C, a photoperiod L:D=16h:8h, and a relative humidity of 50-70% for cultivation , the feed is honey water with a concentration of 5%, and the bee is cultivated until the bee emerges;

[0041] (2) Put the chrysalis chrysalis (see the cultivation method Ⅰ of embodiment 1 chrysanthemum chrysalis for the cultivation method) into the bottom of the 10ml centrifuge tube; insert the female wasp of the cotton bollworm into the centrifuge tube, and the female wasp of the cotton bollworm and the alternative host The ratio of the quantity is 3:1, put a piece of absorbent cotton ball soaked with 5% honey water on the inner wall of the upper part of the centrifuge tube, finish picking bees after 48 hours, r...

Embodiment 3

[0047] The influence of embodiment 3 subculture rearing on the cotton bollworm golden bee

[0048] According to Tenebrio molitor pupae through gradient cooling breeding method (treatment 1), the breeding method of contrast 1, contrast 2, contrast 3 respectively continuous breeding 4 generations, the above-mentioned 1st generation, the 3rd generation of this golden hornet The eclosion rate, male to female ratio.

[0049] Table 3 compares the effects of rearing the golden hornet in subculture

[0050]

[0051] The data in Table 3 shows that: after 6 consecutive generations of breeding by the method of breeding Tenebrio molitor pupae through gradient cooling, the emergence rate of bee species of cotton bollworm A. spp. has increased, and the sex ratio has no significant change.

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Abstract

The invention relates to a method for preventing and controlling hyphantria cunea by using trichomalopsis genalis. The method comprises the following steps: (1) feeding trichomalopsis genalis adults; (2) cultivating tenebrio molitor pupae: transferring tenebrio molitor larvae into an insect breeding box with vent holes, carrying out gradient cooling treatment, and storing the treated tenebrio molitor pupae in a constant temperature box at 4 DEG C in a dark place to obtain the tenebrio molitor pupae for bee inoculation; (3) bee inoculation: heating the tenebrio molitor pupae obtained in step (2) in a reverse manner for bee inoculation, wherein a ratio of the tenebrio molitor pupae to the trichomalopsis genalis female adult is 1:3; (4) managing development of parasitic wasps; and (5) releasing parasitic wasps. In the method, the replacement host tenebrio molitor pupae are adopted to breed the trichomalopsis genalis indoors on a large scale, problems that a success rate of noculation of the replacement host bee is low and the number of bred bees is unstable are solved, meanwhile, a variety of dominant parasitic wasps for preventing and controlling hyphantria cunea is enriched, and a new means and a new method are added for biological prevention and control of the hyphantria cunea.

Description

technical field [0001] The invention relates to the technical field of biological control of pests, in particular to a method for controlling the American white moth by using the cotton bollworm golden beetle. Background technique [0002] The American white moth Hyphantriacunea is a worldwide quarantine object and an important invasive species in my country. Its origin is North America, and then spread to Eurasia one after another. American white moth has the characteristics of strong adaptability and reproductive ability, wide adaptability, difficult control, and fast transmission speed. At present, the species commonly used in biological control is the white moth Chouioiacunea, which can use tussah silkworm in production. Artificial breeding of pupae has played an important role in the control of white moth. However, at present, white moth is still spreading and its distribution area continues to expand. It is necessary to develop and utilize more dominant natural enemie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033Y02A90/40
Inventor 孙守慧张艺馨史俊瑞杨丽元
Owner SHENYANG AGRI UNIV
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