58 results about "Ichneumonidae" patented technology

The invention discloses an RNA (Ribonucleic Acid) interference method for parasitic wasps in lepidoptera larvae, which is characterized in that RNA interference is carried out by using a microinjection method or a nano-material immersion method when the parasitic wasps in the lepidoptera larvae are not separated from hosts or are separated from the hosts to cocoon, so that an RNAi system of the parasitic wasps is successfully constructed, and the defects that the parasitic wasps are small in size in an egg stage, and the parasitic wasps are not damaged in the egg stage can be effectively overcome. And RNAi operation difficulty caused by cocoon wrapping in the pupal stage and short life in the adult stage is avoided. Moreover, the method disclosed by the invention is simple to operate and high in interference efficiency, provides a theoretical basis and a reference effect for further researching gene functions of the parasitic wasps, and has important significance on biological control.

The invention discloses a method for breeding Chouioia cunea Yang through spodoptera frugiperda pupae and application of the method in prevention and control of spodoptera frugiperda, and belongs to the field of biological prevention and control of agricultural pests. Aiming at the parasitic process of Chouioia cunea Yang on spodoptera frugiperda pupae, the suitable age of hosts, the processing mode of host pupae, the physiological status of parasitic wasps, the ratio of inoculated wasps to pupae and environmental conditions are determined, and a set of breeding method suitable for indoor large-scale breeding is constructed. The stability of the method in the multi-generation breeding process is verified through continuous generation breeding. Compared with the traditional host hyphantria cunea, the method has the advantages that the development duration of breeding Chouioia cunea by taking spodoptera frugiperda pupae as the host is obviously shortened, and the breeding efficiency is improved on the premise of ensuring high parasitic rate and offspring yield. When the Chouioia cunea Yang bred by the method is released in the field, the grassland spodoptera exigua can be efficiently prevented and controlled, the dosage of chemical pesticides is reduced, and the method has good ecological benefits and application prospects.

The invention relates to a device for improving the breeding and collecting efficiency of pupa parasitic wasps and a using method, and relates to the technical field of insect breeding. Comprising a parasitic wasp rearing cage and a host containing cage. The host containing cage is arranged on the bottom wall in the parasitic wasp rearing cage, a feeding bin is installed on the top wall in the parasitic wasp rearing cage, a porous drowning prevention interlayer is arranged at the bottom of the feeding bin, holes are formed in the side wall of the parasitic wasp rearing cage, and nylon cylinder gauze is installed at the holes. The parasitic wasp breeding safety, convenience and collection efficiency can be effectively improved, and wasp body loss in the breeding process is reduced.

This invention belongs to the field of agricultural and forestry biotechnology, and relates to a method utilizing... 60 A method to increase the oviposition rate of beet armyworm by Co-γ irradiation was used. Beet armyworm pupae within 12 hours of pupal instar were collected and subjected to effective accumulated temperature treatment. Then, for male beet armyworm pupae, [further methods were employed]. 60 Co-source irradiation treatment; irradiated male beet armyworm pupae and unirradiated female pupae were placed in an incubator to cultivate and emerge as adults. The adults were then transferred to adult rearing cages and supplemented with honey water for nutrition. This invention, through effective accumulated temperature treatment, selects a large number of uniformly developed male beet armyworm pupae as irradiation targets. While ensuring emergence rate and adult lifespan, it significantly increases the egg production of beet armyworms and the yield of test insects. The operation process is simple, safe, and reliable. It provides a large source of production insects for the convenient and rapid use of beet armyworms to produce the biological control agent nucleopolyhedrovirus, and for using the insect as a host to propagate natural enemy parasitic wasps.

The invention discloses an artificial feed for efficient propagation of low-age larva parasitic wasps of prodenia litura. The artificial feed is prepared from the following raw materials: agar powder, soybean meal, sweet potato vines with leaves, yeast powder, wheat germs, sodium benzoate, vitamin C, multivitamins, linseed oil, chlortetracycline hydrochloride soluble powder and the like. The artificial feed is used for feeding 2-5-year-old prodenia litura larvae parasitized by parasitic wasps, and the pupation rate and eclosion rate of the parasitic wasps can both exceed 93%.

The utility model discloses a parasitic wasp breeding device capable of continuously feeding a nutrient solution in the technical field of parasitic wasp breeding, the parasitic wasp breeding device comprises a breeding box and a feeding assembly, and the breeding box comprises a box body and a box cover; the feeding assembly comprises a containing box, the containing box is arranged in the box body, a vertical liquid pipe is arranged on the containing box, a plurality of feeding holes are formed in the liquid pipe, the feeding holes are communicated with the interior of the liquid pipe, and the top end of the liquid pipe penetrates through the box cover to be located outside the feeding box; a cotton ball is placed in the liquid pipe, the cotton ball and the feeding hole are located at the same height, the bottom of the cotton ball is connected with a cotton rope, and the cotton rope extends downwards into the containing box. According to the scheme, the nutrient solution can be continuously supplied to feed parasitic wasps.

The application discloses a method for artificially breeding Sirex gregarivora Cresson and applying the Sirex gregarivora Cresson to biological prevention and control of Anoplophora solenitica. The application determines optimal host size, bee connection ratio, breeding environment and other conditions when the Sirex gregarivora Cresson is artificially bred, can significantly improve parasitization rate of artificial bee breeding and reproduce a large number of parasitic bees in a short period. Meanwhile, conditions suitable for storing adult Sirex gregarivora Cresson are also determined, so that the shelf life of the adult Sirex gregarivora Cresson can be extended to 2 months. The Sirex gregarivora Cresson reproduced through the application is applied to biological prevention and control of Anoplophora solenitica larvae in the wild, and optimal prevention and control effect can be obtained according to equal ratio release of bees and worms, and low cost investment is also considered.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The present application belongs to the technical field of artificial propagation of natural enemy insects and biological control of agricultural pests, and discloses a method for mixed feeding of two kinds of aphid parasitic wasps, which mainly has the following technical features: the steps include breeding of host plants, inoculation and propagation of aphids, obtaining and inoculation of two kinds of aphid parasitic wasp eggs, collection of parasitic wasps, removal of impurities, and separation of the two kinds of parasitic wasps. The method for mixed feeding of two kinds of aphid parasitic wasps provided by the present application inoculates two kinds of parasitic natural enemies, i.e. Aphidius gifuensis and Encarsia spp., at different time periods, improves the parasitic efficiency of single wasps, avoids interspecific competition between adult wasps, collects the two kinds of parasitic wasps at the same time, and saves manpower and material resources.

The invention belongs to the technical field of agricultural biological control, and discloses a parasitic wasp cross-season release method and system based on an ecological island and application. The parasitic wasp cross-season release method based on the ecological island comprises the steps that S1, plants with the trapping effect on target pests are planted in 2-3 point positions per mu on the periphery of an annual crop field; s2, before field planting of the trapping plants, pest prevention and control treatment is carried out on the trapping plants; s3, after the trapping plants are planted, pest traps are arranged at the planting points in a centralized mode, and target parasitic wasps are put in the planting points in a centralized mode. By means of the cross-season release method and system for the parasitic wasps, the overwintering survival rate of the parasitic wasps can be increased to 58.7% from 9.2%, the parasitic rate of target pests in the next year is increased by 32.5%, the annual release frequency is reduced by 1-2 times, and the cost is reduced by 40%.

The present application belongs to the field of bio-technology of agriculture and forestry, and relates to a method for increasing the egg production of Spodoptera litura based on irradiation mode. 60 Co source irradiation treatment; the irradiated Spodoptera litura pupae are cultured in an incubator to emerge, then the adults are moved to an adult rearing cage for rearing, and honey water is provided for nutrition. Through effective accumulated temperature treatment, a large number of Spodoptera litura pupae with consistent development are selected as irradiation objects, which can greatly increase the egg production of Spodoptera litura under the premise of ensuring the emergence rate of Spodoptera litura pupae and the lifespan of adults, increase the production of test insects, and the operation process is simple, safe and reliable, which provides a large amount of production insect source for conveniently and quickly producing biological control agent nuclear polyhedrosis virus by using Spodoptera litura, and expanding the host parasitic wasp by using the insect as host.

The present invention discloses a pentadecane parasitic wasp attractant and its application. Compared with existing pest attractants on the market, the pentadecane attractant provided by the present invention attracts beneficial parasitic wasps, eliminating growers' concerns about attracting more pests to their fields. Furthermore, the more beneficial insects there are, the better the field pest control effect. In addition to the pale-footed braconid wasp, the parasitic wasp attractant of the present invention may not only attract parasitic wasps of the Noctuidae family, but also other types of parasitic wasps, thereby increasing the number of parasitic wasps in the field and improving the parasitic wasp parasitism rate in the field, achieving the effect of parasitic wasps controlling field pests.

The present invention discloses a large-scale production device for Meteorus pulchricornis and its production method. The device includes, from top to bottom: a lid provided with a first screen; an upper box with an open top that mates with the lid and a second screen at the bottom; and a lower box with an open top that mates with the bottom of the upper box. According to the phototactic and upward tropic behaviors and the behavior characteristics of ventral clutching parasitism of Meteorus pulchricornis, the present invention designs a method of relatively isolating the host and the parasitoid above and below respectively by using a screen with a certain mesh number, so that the ovipositor of Meteorus pulchricornis can pierce through the mesh holes to reach the host and complete the oviposition behavior. This is beneficial to quickly separating the host and the parasitoid, and quickly transferring the host from the parasitism box to the breeding box, greatly improving the working efficiency during the parasitism process.

This invention discloses a method for artificially breeding *Mesochrysoma pineatus* to increase the mating rate and obtain more female offspring. The method significantly improves the mating and fertilization rate of female *Mesochrysoma pineatus* by adjusting the airflow and light intensity of the mating environment, thereby increasing the proportion of female offspring. This invention also determines the optimal host body weight, the ratio of infected wasps to bees, and the suitable temperature for parasitism and development during breeding, which can significantly increase the parasitism rate, obtain more offspring, and shorten the development cycle of the offspring, allowing for the reproduction of more parasitic wasps in a shorter time. This invention, while ensuring successful mating of adult *Mesochrysoma pineatus*, improves the parasitism rate of female wasps during artificial breeding, resulting in more offspring, especially female offspring, and shortening the breeding cycle, making it applicable to the large-scale production of this parasitic wasp.

This invention belongs to the field of biological control technology and discloses a fluorescently labeled parasitic wasp tag, including a substrate and a main protective sheet and a secondary support sheet connected to it; the main protective sheet and the secondary support sheet are respectively provided with a first egg tag area and a second egg tag area on their surfaces; a photoluminescent functional layer continuously covers the outer surface of the substrate and covers the area where the fold line is located; a fixing member extends from the secondary support sheet away from the fold line, and has insertion holes arranged in an array along its length; when the main protective sheet is folded along the fold line to the secondary support sheet, the first egg tag area and the second egg tag area form a continuous support surface; the main protective sheet covers the secondary support sheet to form a protective structure with a top opening. This invention adopts a folding self-locking structure, providing reliable sealing protection, preventing insect escape, moisture intrusion, and physical damage. The full-area fluorescent layer has uninterrupted coverage, and the key areas are continuously and conspicuously marked. Gradient insertion holes and radial ribs disperse stress to prevent insect eggs from falling off, and the overall structure is more wear-resistant.

The invention relates to the technical field of parasitic wasp incubation, in particular to a parasitic wasp incubation and rapid extraction device and a use method.The parasitic wasp incubation and rapid extraction device comprises an incubation chamber, and an adult breeding chamber is arranged on the front side of the incubation chamber. According to the parasitic wasp incubation and rapid extraction device, automatic and precise control over the parasitic wasp incubation and rapid extraction process is achieved, coordinated linkage of all parts of the cultivation assembly is achieved through precise driving of an electric push rod, an artificial pupa containing frame can be unfolded and folded according to needs, a suitable space environment is provided for injection and subsequent development of parasitic wasp eggs, and meanwhile the artificial pupa containing frame can be used for containing the parasitic wasps. The movable and sealed design of the closed bin effectively isolates the adult breeding chamber from the incubation chamber, avoids mutual interference of parasitic wasps in different stages, improves the cultivation efficiency, and the arrangement of the warm lamp and the light source attracting device not only provides stable temperature conditions for incubation of parasitic wasp eggs, but also ingeniously guides the behaviors of the parasitic wasps, and improves the survival rate of the parasitic wasps. Precise injection of eggs and smooth separation of adults are achieved.

The utility model discloses a field propagation device for fruit fly pupa parasitic wasps, which relates to the technical field of parasitic wasp breeding and comprises a box body, a box cover, pupa placing mechanisms, a moving mechanism, feeding mechanisms and a water supply mechanism. The moving mechanism is arranged below the food supply mechanism, the top of each moisturizing layer is provided with one pupa placing mechanism, the water supply mechanism is arranged on the inner bottom wall of the box body below the moving mechanism, the device is provided with the multiple layers of supporting frames, the supporting frames have the stretchable characteristic, the space of the device is fully utilized, multiple layers of fruit fly pupae can be placed in the device, and the device is more convenient to use. A large number of hosts are provided for parasitic wasps, and parasitic success rate of the parasitic wasps is improved.

This invention belongs to the field of insecticide ecological safety evaluation technology, and specifically relates to a rapid evaluation method for insecticide safety based on parasitic wasp embryonic development. This invention uses the embryos of the cotton bollworm *Ichthyophthirius multifiliis* as the research object, selecting embryonic development status as the core evaluation indicator. Combining the statistical significance and quantitative change range of this indicator with the control group, a standardized experimental measurement method and a three-level ecological safety classification rule are established. By selecting commonly used agricultural insecticides bromfenac and abamectin, ecological safety evaluations were conducted. Compared with the control group, the number of normal embryos with bromfenac decreased by approximately 17%, classifying it as a medium-risk insecticide; the number of normally developing embryos with abamectin decreased by approximately 23.21%, classifying it as a high-risk insecticide. This application fills the technical gap in the ecological safety evaluation of insecticides at the parasitic wasp embryonic stage, improves the evaluation system for non-target natural enemies, and can be directly applied to pesticide research and development, insecticide screening, and field application guidance, contributing to the green development of agriculture.

The invention discloses a Qinghai grassland caterpillar control management method and system based on parasitic fly breeding and releasing, and the method comprises the steps of parasitic fly provenance collection and breeding, and artificial large-scale breeding and field releasing management, and specifically comprises the following steps: 1, parasitic fly provenance collection and breeding: a) provenance directional collection, the method comprises the following steps: randomly dissecting 4000 caterpillars of the Qinghai grassland in an ecological region of the Qinghai grassland, and screening caterpillars individuals parasitized by exorista and not parasitized by other parasitic wasps for the second time; the invention relates to the technical field of grassland pest biological control, in particular to a Qinghai grassland control management method and system based on parasitic fly breeding and releasing, and aims to realize continuous control of grassland population and zero use of chemical pesticides, and improve the productivity of a grassland by more than 15%.

The application provides a parasitic wasp attractant and application thereof, and belongs to the technical field of pest control. The parasitic wasp attractant provided by the application comprises an active ingredient and an organic solvent, the active ingredient is citral, and the concentration of citral in the parasitic wasp attractant is >2 ng / µL. The application adopts citral as the active ingredient of the parasitic wasp attractant, and citral shows a high and stable attracting effect on parasitic wasps such as aphelinids even under trace conditions, so as to be beneficial to improving the parasitization rate and mortality rate of field pests such as whiteflies (specifically, whitefly nymphs). In addition, the application adopts citral as the active ingredient, citral is not only widely sourced and low in cost, but also is environment-friendly and does not cause harm to non-target organisms, and thus conforms to the development trend of modern green agriculture.

The application discloses a method for breeding of the Tamarixia radiata and application of the Tamarixia radiata in prevention and treatment of the Sibicides brouni, wherein the culture tube containing the paired Tamarixia radiata adult is vertically placed in a culture box for culture, the culture tube is placed in a low-temperature storage environment and is supplemented with food 1-2 times per week, a green bean weevil host is selected, the Tamarixia radiata in the tube is taken out from the low-temperature environment, 30-40 green bean weevils with the green bean weevil hosts are put into each culture tube after the tube cover is opened, the culture tube is closed, the culture tube is placed horizontally in the culture box for mating, the host is regularly replaced after oviposition, the replaced host is placed in a new tube together with the green bean and is placed horizontally in the culture box or in a room temperature environment until the next generation is eclosed. The method can prepare a large number of parasitic wasps, further test host types, select other coleopteran weevil hosts and has potential natural enemy prevention and treatment application value, and is a new measure for prevention and treatment of natural enemies of garden pests.

The utility model relates to the technical field of agricultural planting, and discloses a birch planting insect prevention device which comprises a transparent plate and an insect prevention mechanism, and a fixing mechanism is arranged on the outer side of the insect prevention mechanism. The insect prevention mechanism comprises a supporting plate, a connecting plate is fixedly connected to the inner side of the supporting plate, an insect breeding house is fixedly connected to the inner side of the supporting plate and the inner side of the connecting plate, inserting grooves are formed in one side of the supporting plate and one side of the connecting plate, a plurality of through holes distributed in a linear array mode are formed in the inner walls of the two sides of each inserting groove, and springs are fixedly connected into the through holes. And one end of the spring is fixedly connected with a semicircular clamping block, and a clamping plate is inserted into the inserting groove. According to the birch planting insect prevention device, the insect breeding room is installed on the inner side of the frame and serves as a space for breeding beneficial insects such as ladybirds and parasitic wasps, the beneficial insects are bred in the insect breeding room and help control of the number of the pests, the pests are controlled in a natural mode, dependence on chemical pesticides is reduced, and ecological balance and environmental protection are facilitated.

This invention relates to the field of biological control technology for forest pests, specifically to an automatic field release device and method for parasitic wasps. The device includes a buried component comprising a buried pipe and a storage pipe fixedly connected to the inner wall of the buried pipe. A spiral tube connects the buried pipe and the storage pipe. Multiple first corrugated pipes are located at the bottom of the inner wall of the storage pipe and are connected to the spiral tube. A top plate is fixedly connected to the top of each first corrugated pipe. When the buried pipe is inserted into the soil, the parasitic wasp pupae are kept in a delayed development state. Water from the storage pipe slowly enters the first corrugated pipes under gravity through a micro-flow regulating valve, causing the first corrugated pipes to expand and move the top plate upwards. This causes the top plate to orderly push the multiple wasp pupae from the storage pipe and roll them onto the soil to await hatching, thus achieving the goal of periodic release throughout the entire pupal stage of the pest.

The invention belongs to the field of plant protection and biological control, and particularly discloses a method for raising leaf miner parasitic wasps and a method for biological control by using the leaf miner parasitic wasps. Clematis orientalis is used as a host plant to feed clematis orientalis leaf miners, and then the clematis orientalis leaf miners living in the host plant are used as hosts of parasitic wasps of the leaf miners. According to the method, biological control can be particularly carried out in a greenhouse, the fed clematis orientalis leaf miners are used as host plants to feed the clematis orientalis leaf miners, then the whole hosts of the clematis orientalis leaf miners living in the host plants as leaf miner parasitic wasps are placed in the greenhouse, and the fed parasitic wasps do not need to be additionally collected to be independently released; therefore, the parasitic wasps can be fed and released at the same time in the release stage, the leaf miner can be continuously prevented and controlled for a long time, and practical value is achieved.

The present application relates to a method for increasing the reproductive capacity of Sirex noctilio by water bath treatment of Tenebrio molitor pupae, which comprises preparing a series of necessary materials and equipment, including Tenebrio molitor larvae, culture dishes, absorbent paper, glass pipettes, etc., and then feeding Tenebrio molitor under suitable conditions until pupation. Next, select Tenebrio molitor pupae that have not been hatched for more than 48 hours and have similar quality, and then perform constant temperature water bath treatment at 30 DEG C for 3 minutes to improve their physiological state. The treated Tenebrio molitor pupae are used as parasitic hosts of Sirex noctilio, and the method effectively increases the parasitization rate and success rate of Sirex noctilio on the alternative host Tenebrio molitor pupae, provides high-quality parasitic wasp resources for biological control, helps to reduce the use of chemical pesticides, protects the ecological environment, and realizes the sustainable development of agriculture and forestry.

The invention belongs to the technical field of biopesticides, and particularly discloses a parasitic wasp behavior regulating agent based on plant secondary metabolites. The parasitic wasp behavior regulating agent comprises the following components in percentage by mass: 0.5%-99% of a behavior regulating agent active component; 1%-99.5% of an auxiliary material; the active components of the behavior regulating agent are delta-juniene, cis-jasmonone and 2-tridecanone. The behavior regulating agent provided by the invention can induce parasitic wasps and improve the ability of the parasitic wasps to search and position target pests, so that the parasitic efficiency of the parasitic wasps is improved, and therefore, effective biological prevention and control of the pests can be realized, the application of chemical pesticides can be reduced, and the safety of vegetable products can be ensured.

The invention belongs to the technical field of biological control of agricultural pests, and particularly relates to a method for comprehensively evaluating the storage quality of low-temperature storage parasitic wasps and application. Through real-time fixed-point dissection, the effective storage starting point of the cotton bollworm odontolimus starts from 24 + / -4 hours of cocooning; establishing a pupal period effective accumulated temperature equation N = 112.46 / (T-7.94), predicting the developmental duration at the temperature of 7.94-25 DEG C, and further adjusting the developmental duration according to field requirements; an equation Y = 0.8611 X-5. 968 for comprehensively evaluating the pest control ability of the low-temperature storage parasitic wasps is established and used for adjusting the field release number of the parasitic wasps during storage at different temperatures so as to guarantee a good field pest control effect; a storage quality standard for comprehensively evaluating the parasitic wasps is established, and the comprehensive storage quality of the parasitic wasps at different temperatures is determined. The result shows that the comprehensive storage quality at 20 DEG C is the highest, the storage time is prolonged, and the biological control progress of lepidoptera noctuidae is promoted.

The invention belongs to the field of plant protection, and particularly relates to a diapause control gene AjFoxO of apis cerana and application of the diapause control gene AjFoxO. According to the application disclosed by the invention, the key gene AjFoxO for diapause control of the small apis belly bees is found through screening, and the influence of the AjFoxO gene on the diapause behavior of the small apis belly bees is verified through an addition test of RNAi (Ribonucleic Acid Interfere) and exogenous juvenile hormone. The method can be used for diapause control of the small apis belly bees, so that the shelf life of parasitic wasp products is controlled, and the optimal economic benefit is achieved. And the method can also be used for breeding and screening the variety of the small apis belly so as to obtain the variety with long diapause period and serve for biological prevention and control of agriculture and forestry.

The invention relates to the field of parasitic wasp control, in particular to a method for efficiently controlling offspring sexual ratio and individual size of parasitic wasps by using artificial eggs, which comprises the following steps of: 1, preparing an artificial egg template with proper size; 2, a film forming material is flatly laid in the artificial egg template in the step 1, a rod-shaped object with the corresponding size is used for being matched with the artificial egg template to make a sphere or a hemisphere in a plastic film, and egg liquid is poured into the shaped film for plastic packaging; 3, the parasitic wasps are inoculated into the artificial eggs obtained in the step 2, and the parasitized artificial eggs are placed in an incubator to be developed till eclosion of the parasitic wasps. By changing the size of the artificial eggs, the purpose of controlling the sex proportion and the individual size of the parasitic wasp offspring is achieved, the potential risk of chemical residues on wasp body health or environment is avoided, the process is simpler, the cost is lower, and the method is easier to implement on a standardized production line.