Indoor propagation method for steep cocoon manilensis

A technique of wasps and steep breasts, applied in the field of reproduction of parasitic wasps, can solve problems such as inability to multiply

Pending Publication Date: 2022-01-04
HUBEI BIOPESTICIDE ENG RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the application of parasitoids in China is mainly Trichogramma, and the level of artificial propagation and release of Trichogramma to control corn borer is relatively high. There are few domestic and foreign research reports on the indoor propagation technology of Stenobreasts Manila
The problem in the prior art is that parasitoids c

Method used

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  • Indoor propagation method for steep cocoon manilensis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The present embodiment provides a kind of indoor propagation method of Steepbreasts Manila, which specifically includes the following steps:

[0027] (1) Take the pupae of Stemoptera Manila for indoor breeding. After the parasitic wasp emerges, the adult parasitic wasp on the third day of emergence is used as a kind of bee; the temperature of indoor breeding is 25 ± 1 ° C, and the relative humidity is 70-80 %, the photoperiod is L:D=14:10h;

[0028] (2) Take a centrifuge tube with a 1ml cover pierced with a 1-3 instar larva of the host beet armyworm, and insert a male and female adult parasitoid on the 3rd day of eclosion, and then place the centrifuge tube in an artificial climate chamber The co-feeding of parasitoids and hosts was carried out, and the hosts were replaced every 24 hours until all the female parasitoids died, and a total of 9 consecutive new hosts were replaced; the conditions in the artificial climate chamber were: the temperature was 25 ± 1 ° C, relat...

Embodiment 2

[0032] The difference between this embodiment and embodiment 1 is that in step (2), a total of 10 new hosts are continuously replaced.

Embodiment 3

[0034] The difference between this embodiment and embodiment 1 is only: the adult parasitoid wasp that has emerged for the 3d day is used as a kind of bee, and the others are completely the same as embodiment 1.

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Abstract

The invention relates to an indoor propagation method of steep cocoon manilensis. The indoor propagation method comprises the following steps: firstly, indoor breeding is conducted on pupae of the steep cocoon manilensis to obtain first-generation breeding bees, then the first-generation breeding bees are inoculated into an insect breeding cage, and then a turnover box inoculated with host beet armyworm larvae is put into the insect breeding cage to perform co-breeding of parasitic bees and hosts; after a period of time, the turnover box is taken out and placed in a new insect breeding cage, the original insect breeding cage is replaced with a new host for continuous parasitism, and the steps are repeated until all female parasitic wasps die; and parasitic wasp cocoons which are cocooned and developed to the pupal stage after parasitism are collected, and the parasitic wasp cocoons are cultured to eclosion to serve as breeding wasps to be used for breeding next-generation parasitic wasps. According to the method, beet armyworm larvae serve as the hosts, the steep cocoon manilensis can parasitize and generate a large number of offspring and can be stored in a pupa form at low temperature and normal temperature, indoor large-scale breeding of the steep cocoon manilensis is achieved, an insect source is provided for field release application of the steep cocoon manilensis, and technical support is provided for biological control of noctuid pests.

Description

technical field [0001] The invention belongs to the technical field of reproduction of parasitic wasps, and in particular relates to a method for indoor propagation of Brachydontoma manila. Background technique [0002] Beet armyworm (Spodoptera exigua Hübner) is an important pest with a very wide range of occurrence. It mainly harms 138 species of crops in 35 families, 108 genera, such as vegetables, tobacco, cotton, corn, soybeans, etc. The pests of this family feed on leaves and stems of plants with larvae. stalks, resulting in a substantial reduction in crop yields. At present, chemical pesticides are still the main means of controlling sugar beet night. As people pay more attention to food safety, the demand for pollution-free green food has increased, and the use of chemical control has made problems such as excessive plant pesticide residues, increased resistance of pests, and the rise of secondary pests to major pests become increasingly serious. Natural enemies in...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 王蓓蓓曹春霞黄大野朱志刚张志刚李飞
Owner HUBEI BIOPESTICIDE ENG RES CENT
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