Indoor propagation method for steep cocoon manilensis
A technique of wasps and steep breasts, applied in the field of reproduction of parasitic wasps, can solve problems such as inability to multiply
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Embodiment 1
[0026] The present embodiment provides a kind of indoor propagation method of Steepbreasts Manila, which specifically includes the following steps:
[0027] (1) Take the pupae of Stemoptera Manila for indoor breeding. After the parasitic wasp emerges, the adult parasitic wasp on the third day of emergence is used as a kind of bee; the temperature of indoor breeding is 25 ± 1 ° C, and the relative humidity is 70-80 %, the photoperiod is L:D=14:10h;
[0028] (2) Take a centrifuge tube with a 1ml cover pierced with a 1-3 instar larva of the host beet armyworm, and insert a male and female adult parasitoid on the 3rd day of eclosion, and then place the centrifuge tube in an artificial climate chamber The co-feeding of parasitoids and hosts was carried out, and the hosts were replaced every 24 hours until all the female parasitoids died, and a total of 9 consecutive new hosts were replaced; the conditions in the artificial climate chamber were: the temperature was 25 ± 1 ° C, relat...
Embodiment 2
[0032] The difference between this embodiment and embodiment 1 is that in step (2), a total of 10 new hosts are continuously replaced.
Embodiment 3
[0034] The difference between this embodiment and embodiment 1 is only: the adult parasitoid wasp that has emerged for the 3d day is used as a kind of bee, and the others are completely the same as embodiment 1.
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