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Method for increasing parasitic rate of glyphodes pyloalis parasitic wasps by using methyl caffeate

A technology of methyl caffeate and parasitic wasps, which is applied in the field of biological control of agricultural pests, can solve problems such as lack and unsuccessful promotion, and achieve the effects of improving parasitic rate, low cost and simple production.

Pending Publication Date: 2021-07-30
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, due to the lack of effective parasitoid products, the control of parasitoids in the larval stage of lepidopteran pests has not been successfully promoted.

Method used

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  • Method for increasing parasitic rate of glyphodes pyloalis parasitic wasps by using methyl caffeate
  • Method for increasing parasitic rate of glyphodes pyloalis parasitic wasps by using methyl caffeate
  • Method for increasing parasitic rate of glyphodes pyloalis parasitic wasps by using methyl caffeate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Using methyl caffeate to improve the parasitism rate of the mulberry borer parasitoid mixed-chamber braconid

[0017] A 0.1wt.%-4.0wt.% methyl caffeate solution was prepared by using absolute ethanol as a solution. Using the leaf disc method, the mulberry leaves were made into a leaf disc with an area of ​​about 10. The leaf discs were respectively immersed in caffeic acid methyl ester solutions of different concentrations for 1 minute, then taken out and dried for feeding by the 3rd instar mulberry borer larvae. Take clear water as a control. On the first day after the feeding was completed, the mixed-chamber wasps were parasitized. The parasitic environment is a round transparent plastic box. There is a round hole in the middle of the lid, which is sealed with mesh cotton for ventilation. A hole for introducing parasitic wasps is provided on the side of the circular plastic box; a thin layer of agar is spread on the bottom of the circular plastic box, an...

Embodiment 2

[0020] Example 2 Using methyl caffeate to improve the parasitism rate of the mulberry borer parasitoid mixed-chamber braconid

[0021] Caffeic acid methyl ester solution preparation method is with embodiment 1. In the leaf disc method, the area of ​​the mulberry leaf disc used is about 10, 15, 20 cm 2 . The method that leaf dish is processed with methyl caffeate solution is with embodiment 1. The leaf discs of different areas were provided to the 3rd instar mulberry borer for feeding, and after the feeding was completed, they were used to parasitize the mixed chamber wasps, and the parasitizing method was the same as in Example 1. Known from Table 2, mulberry borer larvae feed on 20cm 2 The parasitism rate of mixed chamber wasps was 75.4%±1.8% on the mulberry leaves containing caffeic acid methyl ester.

[0022] Table 2 The parasitism rate (%) of the mixed-chamber braconid parasitizing the mulberry borer that feeds on the mulberry leaves containing caffeic acid methyl este...

Embodiment 3

[0024] Example 3 Utilizing methyl caffeate to increase the parasitism rate of the mixed-chamber braconid of the mulberry borer parasitoid

[0025] The preparation method of caffeic acid methyl ester solution and the method of soaking leaves are the same as in Example 1. With 3 instar mulberry borer as host. The time for the mulberry borer larvae to feed on caffeic acid methyl ester is 6, 8, 10, 12 hours. The area of ​​the mulberry leaf dish is 20cm 2 . After feeding, the mulberry borer larvae were provided to the mixed-chamber braconid for parasitism. Take clear water as a control. It is known from Table 3 that after the mulberry borer larvae ate the mulberry leaves containing caffeic acid methyl ester for 8 hours, the parasitism rate of the mixed-chamber braconids was 77.6%±9.7%.

[0026] Table 3 The parasitism rate (%) of the mixed-chamber braconid parasitizing the mulberry borer feeding on the mulberry leaves containing caffeic acid methyl ester

[0027]

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Abstract

The invention discloses a method for increasing the parasitic rate of glyphodes pyloalis parasitic wasps by using methyl caffeate. The method comprises the following steps: preparing a methyl caffeate solution; providing mulberry leaves containing the methyl caffeate for glyphodes pyloalis larvae to eat; and providing the glyphodes pyloalis larvae which eat the mulberry leaves containing the methyl caffeate for the mixed chamber to parasiticus. When the method is used for breeding the mixed-chamber cotesia, the parasitism efficiency is high, the loss is small, the operation is simple and convenient, the cost is low, the problem that leaf rolling of larvae of the stem borer family is not beneficial to parasitism of the parasitic wasps is solved, large-scale indoor production of mulberry pest mixed-chamber cotesia parasitic wasps is facilitated, and an important foundation is laid for field biological control work of the glyphodes pyloalis.

Description

technical field [0001] The invention belongs to the technical field of biological control of agricultural pests, and relates to a method for increasing the parasitic rate of parasitic wasps of the major mulberry pest, mulberry borer. Background technique [0002] The mulberry moth Glyphodes pyloalis belongs to the Lepidoptera of Lepidoptera and Pyralida of the family Pyralidae. It is one of the major pests on mulberry trees in my country and Southeast Asian countries. It can grow rapidly, and the phenomenon of overlapping generations is obvious, causing a large area of ​​mulberry leaves to be eaten and damaged, and in serious cases, causing mulberry leaves to fail. In addition, the feces of mulberry borer larvae can easily pollute mulberry leaves, resulting in a decline in the quality of mulberry leaves. The larvae are also bridge hosts for many pathogenic microorganisms in silkworms. After eating mulberry leaves contaminated by mulberry borers, silkworms are very susceptible...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K50/90A23K10/30A23K20/111
CPCA01K67/033A23K50/90A23K10/30A23K20/111
Inventor 严孟文邢晓蓉杨学钧盛晟王俊
Owner JIANGSU UNIV OF SCI & TECH
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