Artificial propagation method for parasitic wasps of bactrocera tau larvae

A technology for artificial reproduction and parasitic wasps, applied in the direction of animal husbandry, etc., can solve the problems of reducing the emergence rate and vitality of parasitic wasps, affecting the parasitic wasp parasitic pupae, and reducing the number of passing through gauze, etc., and achieves easy large-scale breeding, low cost, artificial less distracting effect

Inactive Publication Date: 2021-01-29
ANSHUN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this technical solution are: the method is cumbersome to operate, and the fruit flies with strong antifreezing ability will quickly recover and escape, and it needs to be frozen again to separate the fruit flies from the parasitic pupae
When the fruit flies are suddenly stunned by low temperature in the freezer, it may affect the parasitic wasp parasitic pupae, reduce the emergence rate and vitality of the parasitic wasp, and due to the sudden low temperature treatment of the parasitic pupa in the early stage, the ability of the parasitic wasp to emerge through the gauze net will be reduced and stay dead in the net

Method used

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  • Artificial propagation method for parasitic wasps of bactrocera tau larvae
  • Artificial propagation method for parasitic wasps of bactrocera tau larvae
  • Artificial propagation method for parasitic wasps of bactrocera tau larvae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: 3d larvae parasitized by parasitic wasps of the larvae of Bactrocera cerevisiae

[0075] 1. Breeding of Bactrocera japonica adults

[0076] Weigh 100-120 g of pupae in a plastic box and put them into insect cage C. Provide 300-400 g of adult food for Bactrocera subtilis after eclosion, place a water-containing nano-sponge on the top of the cage, add water every 2 days, and cultivate at a temperature of 25±2°C. The relative humidity is (65±5)%, and the photoperiod is L:D=12:12.

[0077] 2. When the Bactrocera japonica egg-collecting insect cage C is expanded and raised to the egg-laying high bee stage, the glass culture dish (diameter φ9.0cm) is filled with artificial feed A and placed in the breeding cage to collect eggs 1 ~2h, 4~5 dishes / cage.

[0078] 3. Bactrocera larvae rearing

[0079] Add 300-500g of artificial feed C to the bottom of a 2L black rectangular plastic box, pour the eggs and feed of Bactrocera subtilis in the above-mentioned culture dish...

Embodiment 2

[0093] Embodiment 2, the parasitoid 4d larva of the parasitoid wasp of the larva of Bactrocera cerevisiae

[0094] 1. The parasitic effect of 5 parasitic wasps on the 4d larvae of Bactrocera spp.

[0095] (1) Breeding, egg collection and larval rearing of Bactrocera spp.

[0096] The larvae of Bactrocera cerevisiae were reared according to the steps 1 to 3 in Example 1, except that the larvae were reared to the 4d age.

[0097] (2), 4d South Asian fruit fly larva parasitism

[0098] Take each 5 female and male parasitoids of eclosion in the insect cage E (the specification of the insect cage E is length * width * height is 15 * 15 * 15cm, the front of the described insect cage E is a transparent plastic film, and the back is a tube with a sleeve. white gauze, the bottom is white opaque plastic, and the rest are gauze), in the cage, use 75mL black plastic box with 10% honey water, drill a hole in the center of the box cover, cotton, until the age of female parasitoid bees to ...

Embodiment 3

[0113] Embodiment 3, indoor large-scale breeding of the parasitic wasps of the larvae of Bactrocera cerevisiae

[0114] 1. Breeding of Bactrocera japonica adults

[0115] The eclosion of Bactrocera subtilis was placed in pitcher cage A, the culture temperature was 25±2°C, the relative humidity was 65±5, the photoperiod was L:D=12:12, and adult feed and water were provided.

[0116] 2. South Asian fruit fly multiplication and large cage domestication

[0117] When raising Bactrocera subtilis to the oviposition period, fill a glass petri dish (diameter φ9.0cm) with artificial feed A, place it in insect cage A to collect eggs for 8-10 hours, take out the petri dish, and place the egg-containing feed in a Put the plastic plate on the larva culture frame in the plastic plate with artificial feed C, the culture temperature is 25±2°C, the relative humidity is 65±5, the photoperiod is L:D=12:12; Fine sand with a water content of 2-4% is used to collect larvae for pupation. The fine ...

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Abstract

The invention relates to an artificial propagation method for parasitic wasps of bactrocera tau larvae. The method comprises the steps of collection of bactrocera tau and adult parasitic wasps of bactrocera tau larvae, feeding and domestication of the parasitic wasps of the bactrocera tau larvae, feeding of the adult bactrocera tau, expanding propagation and spawning domestication of bactrocera tau, egg collection of the bactrocera tau, expanding propagation of the bactrocera tau larvae, parasitism of the parasitic wasps of the bactrocera tau larvae on the bactrocera tau larvae, larva feedingand pupation after parasitism, parasitic pupae collection and parasitic wasp eclosion separation, and rejuvenation of the bactrocera tau and larva parasitic wasps. By adopting the method disclosed bythe invention, the growth and development of the bactrocera tau and the parasitic wasps are not influenced by residual pesticides, and the host searching capability of the parasitic wasps in the fieldis kept stable; after being collected, the bactrocera tau pupae where the parasitic wasps are parasitic do not need freezing treatment, damage to the bactrocera tau pupae where the parasitic wasps are parasitic is little, the separation effect of the bactrocera tau and the parasitic wasps is good, and the method has the advantages of being easy to operate, capable of saving time, little in humaninterference, high in parasitic rate, easy to breed on a large scale and particularly suitable for application and popularization.

Description

technical field [0001] The invention belongs to the technical field of biological control of agricultural pests, and in particular relates to an artificial propagation method of a parasitoid wasp of the larvae of Bactrocera cerevisiae. Background technique [0002] Lepidoptera Bactrocera (Zeugodacus) tau Walker is one of the main pests that damage melons, fruits and vegetables, and it is currently causing damage in all provinces and regions in southern my country. Bactrocera melons in natural environments Bactrocera (Zeugodacus) cucurbitae Coquillett competes for the same food resources. Due to its short growth cycle and fast larval development, it has replaced melon fly as the dominant species in this area. At present, in some areas of Guizhou and Yunnan, in the fields of fruits and vegetables caused by Bactrocera larvae, several kinds of parasitoids of Bactrocera larvae have been collected. There is no literature report. [0003] The closest prior art to the larva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 敖国富刘天雷张玉波从春蕾孙翠英杜蕾
Owner ANSHUN UNIV
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