Method for artificially feeding parasitic wasps by taking mulberry pyralids as hosts

A technology for parasitic wasps and mulberry borers, which is applied in the field of biological control of agricultural pests, can solve the problems of undiscovered artificial large-scale breeding methods of S. Low, easy to make effects

Pending Publication Date: 2021-06-29
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the artificial large-scale rearing method of the mole mole that takes the mulberry borer as the host
Although there are reports of feeding methods for parasitic wasps that use Lepidopteran insects such as Spodoptera litura and Beet Spodoptera as hosts, there are some characteristics such as low utilization efficiency of parasitic wasps and mothers, and no moths as hosts (Tao Min, 2019; 2010; Sheng, 2017), especially after transfer to the host, it is still worth exploring whether the wasps obtained from other host insects are suitable for parasitizing Mulberry

Method used

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  • Method for artificially feeding parasitic wasps by taking mulberry pyralids as hosts
  • Method for artificially feeding parasitic wasps by taking mulberry pyralids as hosts
  • Method for artificially feeding parasitic wasps by taking mulberry pyralids as hosts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Artificial Breeding of Sapphire spp. with Mulberry Borer as Host

[0016] The mulberry borer larvae were collected from the field during the blooming period of the mulberry borer. The collection object is the mulberry borer larvae in the middle and lower leaves of each mulberry tree. The collected mulberry borer larvae ranged from 1 to 5 instars. 100 mulberry borers were collected at each stage, and the collection was repeated 3 times. The collected mulberry borer larvae were brought back to the laboratory for rearing. The mulberry borer larvae of each age were raised separately in small plastic cups and fed fresh mulberry borer every day. When the parasitic wasp larvae crawled out of the mulberry borer and cocooned, the parasitic bee cocoons were collected. The parasitism rate of mulberry borer in each stage is shown in Table 1, and the parasitism rate of 2-4 instar mulberry borer larvae was the highest with no significant difference. There was no signifi...

Embodiment 2

[0019] Example 2 Artificial Breeding of Sapphire spp. with Mulberry Borer as Host

[0020] The artificial propagation experiment was carried out indoors with the mulberry moth crawled out from the mulberry borer collected in the field as the mother bee. The 6-8-day-old female bee was used as the selected wasp. Set two variables, the age of the host and the density of the mulberry borer in the parasitic box, and set the combination of different ages and host densities according to the pairwise combination. The density of borer is 5 heads, 25 heads and 50 heads per box. Considering the size of the parasitic box and the size difference of the larvae of different ages of the mulberry borer, the specific combination method is shown in Table 2. The parasitic time is 24 hours. Each treatment was repeated 3 times. Table 2 shows that there are significant differences in the indoor parasitism rate of M. mori to Mulberry Borer under different host age and density combination conditio...

Embodiment 3

[0024] Example 3 Artificial Breeding of Sapphire spp. with Mulberry Borer as Host

[0025] The parasitic combinations of Mulberry borer larvae of different ages and densities were set up, and the effects of different parasitic durations on the parasitic rate were investigated. The 6-8-day-old female bee was used as the selected wasp. It can be seen from Table 3 that the parasitic duration has a significant effect on the indoor parasitism rate of P. And the duration of parasitism is closely related to the age of each host and the combination of density and treatment. When the 2nd instar mulberry borer was 35 heads per box, the parasitism rate was the highest for 16 hours; 24 hours, the parasitic rate is the highest.

[0026] Table 3 Indoor parasitism rate (%) under different parasitism conditions

[0027]

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Abstract

The invention relates to a method for artificially feeding parasitic wasps by taking mulberry pyralids as hosts. The method comprises the following steps of: collecting mulberry pyralid larvae from a field, putting the mulberry pyralid larvae into a container paved with mulberry leaves, and collecting parasitic wasp cocoons after the parasitic wasp larvae get out of the hosts to cocoon; placing the parasitic wasp cocoons in glass test tubes, and after eclosion of the parasitic wasps, feeding the parasitic wasps with hydromel; adopting raised parasitic wasp adults for parasitism, and during parasitism, utilizing a transparent container as a spawning site, and inoculating the mulberry pyralid larvae for parasitism of the parasitic wasps; after parasitism is finished, collecting the mulberry pyralid larvae and the parasitic wasps respectively, feeding the mulberry pyralid larvae until the offspring wasps of the parasitic wasps get out of the hosts, and allowing the parasitic wasps to be used for subsequent parasitism again. When the method is used for breeding meteorus pulchricornis, the parasitism efficiency is high, the loss is small, the operation is simple and convenient, the cost is low, the host source of the meteorus pulchricornis is expanded, the indoor large-scale production of mulberry pest parasitic wasp meteorus pulchricornis is facilitated, and a good foundation is laid for the field biological control work of mulberry pyralids.

Description

technical field [0001] The invention belongs to the technical field of biological control of agricultural pests, and relates to a method for artificially raising parasitoid bees with mulberry borers as hosts. Background technique [0002] There are many kinds of mulberry pests, and some pests have become more and more serious in recent years, seriously threatening the development of sericulture industry. The mulberry borer Glyphodes pyloalis (Walker) is one of the major pests of mulberry trees. It has a strong ability to cope with catastrophe. Even if the source base of the mulberry is very low, once the environment is suitable, it can quickly break out into disasters in a short period of time, resulting in reduced or even no harvest of mulberry leaves. At the same time, mulberry borer can also spread a variety of silkworm pathogenic microorganisms, affecting the safety of silkworm breeding (Zhao Xuhua, 2013; Wu Fuan, 2015). Due to the covert damage caused by leaf rolling o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 邢晓蓉严孟文陈洪超杨学钧耿丽恬沈翔宇吴福安王俊盛晟
Owner JIANGSU UNIV OF SCI & TECH
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