Large-scale production method of tamarixia radiate

The invention relates to a technology for the production method of the bee-glazed wasp, which is applied in the direction of animal husbandry and the like, and achieves the effects of improving the reproductive capacity and the production capacity of parasitic wasps, low energy consumption, and solving environmental problems.

Pending Publication Date: 2021-04-09
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for large-scale production of citrus psyllids with large net harvest and high female ratio. The present invention solves the problems in the existing production technology of citrus psyllids and realizes the large-scale production of citrus psyllids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Construction of parasitoid production workshop

[0028] 1. Build a budding room. Light-transmitting and rain-proof, protected by 60-mesh insect-proof nets, used for the production of citrus buds. The light intensity is 3000LUX, the temperature is 25-30°C, the RH is 50%-70%, and the light L:D=14:10.

[0029] 2. Build a citrus psyllid breeding room. Light-transmitting and rain-proof, protected by 60-mesh insect-proof nets, controlling the temperature of the breeding room, installing breeding cages in the breeding room, used for the production of citrus psyllid nymphs, the light intensity is 3000LUX, the temperature is 25-30°C, RH50%-70% , Lighting L:D=14:10. The citrus psyllid breeding cage is supported by a stainless steel pipe, and the bottom is a polypropylene plate. The cage specifications are 110 cm long, 90 cm wide, and 100 cm high.

[0030] 3. Build a parasitoid breeding room. A parasitic bee breeding cage is installed in the breeding room for the productio...

Embodiment 2

[0050] Embodiment 2: the inoculation ratio selection of citrus psyllid

[0051] The number of citrus psyllids produced is different at different inoculation ratios between citrus psyllids and young shoots of tangerines. The inoculation ratio can be selected according to production needs and carrier plant conditions.

[0052] The present embodiment is basically the same as Example 1, except that the citrus psyllid is inoculated: the number of access of the young shoots of the tangerine is as shown in table 1:

[0053] Table 1 The number of citrus psyllids harvested at different inoculation ratios

[0054] Citrus Psyllid: Annual Tangerine Sprouts The number of citrus psyllids harvested per plant (after 18 days) 1:1 151.33±9.45 2:1 247.67±8.39 3:1 269.33±4.51 4:1 205.33±20.51

[0055] Usually, the new shoots of each annual tangerine are controlled at about 40 branches, and the time for harvesting new eclosion citrus psyllids is calculated on th...

Embodiment 3

[0059] The selection of the inoculation ratio of embodiment 3 bright belly glaze wasps

[0060] The citrus psyllid nymphs on the carrier plants are required to be neatly developed and mostly 4-5 instars. There are about 250 psyllid nymphs on each plant, and 5 carrier plants are placed in each breeding cage. The present embodiment is basically the same as Embodiment 1, except that the number ratio of access to the bright belly glaze wasp (female to male ratio is 4:1) and the citrus psyllid nymph is different, specifically as shown in Table 2:

[0061] Table 2 The number of bees produced by different access ratios

[0062] Bright-bellied wasp: citrus psyllid nymph Ratio of female parasitoids (%) The number of bright-bellied glaze bees harvested per cage (head) 1﹕5 61.25±2.53 811.55±21.76 1﹕10 73.63±5.39 766.75±14.14 1﹕15 76.42±4.26 602.3±30.58 1﹕20 77.03±10.62 487.31±24.37

[0063] As can be seen from Table 2, when the inoculation ratio...

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Abstract

The invention discloses a large-scale production method of tamarixia radiate. The method comprises the steps that s1, carrier plants are managed, tender shoots are cultivated, and the carrier plants are calamondin; s2, diaphorina citri is inoculated to the carrier plants in a diaphorina citri breeding cage, and nymphs are bred; s3, the carrier plants with the 4-5-age diaphorina citri nymphs are moved into a parasitic wasp breeding cage, tamarixia radiate adults are put into the parasitic wasp breeding cage, the inoculation quantity ratio of tamarixia radiate to the diaphorina citri nymphs is 1: 10-20, and the tamarixia radiate adults are bred and collected; and s4, the carrier plants are cleaned and recovered. The carrier plants developed by the method are calamondin, the carrier plants shorten the growth cycle, the breeding capacity of the diaphorina citri and the production capacity of parasitic wasps are greatly improved, and the net yield and the female ratio of the tamarixia radiate are effectively improved according to the inoculation proportion of calamondin+tamarixia radiate to the diaphorina citri nymphs.

Description

Technical field: [0001] The invention belongs to the technical field of biological control of agricultural pests, and in particular relates to a method for large-scale production of the bright-bellied wasp. Background technique: [0002] Citrus psyllid (Diaphorinacitri Kuwayama) is the main pest of citrus plants in the young shoot stage. It sucks the plant juice through the piercing-sucking mouthparts, and lays eggs on the new shoots, often causing the new shoots to wither and deform, and excrete White honeydew, which causes sooty rot. What's more serious is that, as the main insect vector for the transmission of Huanglongbing bacteria, it often leads to the outbreak of Huanglongbing disease in orchards. After a few years, the diseased trees infected with Huanglongbing will lose their economic value. The development of the industry, at present, has not yet found an effective method for the treatment of Huanglongbing. [0003] For a long time, chemical drugs have been used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 毛润乾修宝林黄承志
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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