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Method for propagating telenomus remus by using fresh-keeping spodoptera frugiperda egg masses

A technology for Spodoptera frugiperda and Spodoptera frugiperda, applied in the field of agriculture and forestry biology, can solve the problems of limited egg production, difficulty in accumulating egg sources, etc., achieves simple operation process, low material cost, and avoids chemical and physical inactivation operations. Effect

Active Publication Date: 2020-10-13
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, limited by the number of eggs laid by Spodoptera frugiperda and the rapid hatching of eggs, it is difficult to accumulate a certain scale and quantity of egg sources with consistent physiological indicators under indoor conditions for the expansion of Spodoptera frugiperda.

Method used

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  • Method for propagating telenomus remus by using fresh-keeping spodoptera frugiperda egg masses

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Comparison scheme
Effect test

Embodiment 1

[0034] A kind of fresh-keeping Spodoptera frugiperda ovum of the present invention is used for the method for multiplying Spodoptera spp., comprising the following steps:

[0035] (1) Kraft paper is used as a carrier medium for adult fall armyworm eggs

[0036] a. Introduce the newly emerged adults of Spodoptera frugiperda into a 30×30×30cm insect cage, and put in cotton balls containing 10% honey water to supplement nutrition. After 2 days, hang a piece of kraft paper about 15×15cm in the center of the top of the cage It is used for trapping and laying eggs, as a carrier medium for trapping adults of Spodoptera frugiperda to lay eggs;

[0037] b. Replace the kraft paper every morning, check and cut off the piece of paper containing the egg mass at 1 cm from the outer edge of the egg mass, rinse the egg mass with a syringe containing normal saline and dry it to remove possible pathogens, and dry it for storage;

[0038] (2) Fresh-keeping treatment process of Spodoptera frugip...

Embodiment 2

[0047] The method for the preservation of the fresh-keeping Spodoptera frugiperda ovum of the present invention for multiplying Spodoptera black egg bee comprises the following steps:

[0048] (1) Kraft paper is used as a carrier medium for adult fall armyworm eggs

[0049] a. Introduce the newly emerged adults of Spodoptera frugiperda into a 30×30×30cm insect cage, and put in cotton balls containing 10% honey water to supplement nutrition. After 2 days, hang a piece of kraft paper about 15×15cm in the center of the top of the cage It is used for trapping and laying eggs, as a carrier medium for trapping adults of Spodoptera frugiperda to lay eggs;

[0050] b. Replace the kraft paper every morning, check and cut off the piece of paper containing the egg mass at 0.5cm from the outer edge of the egg mass, rinse the egg mass with a syringe containing pure water and dry it to remove possible pathogens, and dry it for storage;

[0051] (2) Fresh-keeping treatment process of Spodop...

Embodiment 3

[0060] The method for the preservation of the fresh-keeping Spodoptera frugiperda ovum of the present invention for multiplying Spodoptera black egg bee comprises the following steps:

[0061] (1) Kraft paper is used as a carrier medium for adult fall armyworm eggs

[0062] a. Introduce the newly emerged adults of Spodoptera frugiperda into a 30×30×30cm insect cage, and put in cotton balls containing 10% honey water to supplement nutrition. After 2 days, hang a piece of kraft paper about 15×15cm in the center of the top of the cage It is used for trapping and laying eggs, as a carrier medium for trapping adults of Spodoptera frugiperda to lay eggs;

[0063] b. Replace the kraft paper every morning, check and cut off the piece of paper containing the egg mass at 0.8cm from the outer edge of the egg mass, rinse the egg mass with a syringe containing normal saline and dry it to remove possible pathogens, and dry it for storage;

[0064] (2) Fresh-keeping treatment process of Spo...

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Abstract

The invention discloses a method for propagating telenomus remus by using fresh-keeping spodoptera frugiperda egg masses. Kraft paper is mainly used as a bearing medium for the spodoptera frugiperda egg masses, the egg masses are stored under the limiting conditions such as airtightness, light shielding and constant low temperature, hatching of the spodoptera frugiperda eggs is inhibited, meanwhile, the method ensures that the telenomus remus can parasitize on the egg masses within a long time, the purpose of intensively propagating a large number of telenomus remus populations is achieved, and the method conveniently releases parasitic wasps in the field at the right time so as to achieve the biological control effect of preventing and controlling the spodoptera frugiperda. The method issimple in operation process, safe and reliable, and provides a large number of parasitic wasp sources are provided for conveniently and rapidly utilizing the spodoptera frugiperda eggs to massively propagate the telenomus remus and applying the biological prevention and control technology for preventing and controlling the spodoptera frugiperda eggs.

Description

technical field [0001] The invention belongs to the field of agriculture and forestry biotechnology, and relates to a method for preserving the eggs of host pests and multiplying their natural enemy parasitoids in a large amount indoors, in particular to a method for preserving fresh eggs of Spodoptera meadows and using them to multiply the natural enemies Spodoptera in large quantities The method of the black egg bee. Background technique [0002] Spodoptera frugiperda, commonly known as Fall armyworm (Fall armyworm), belongs to the Lepidoptera Noctuidae family Spodoptera genus, native to tropical and subtropical regions of America, is a major dangerous migratory that seriously threatens agricultural production Fly pests. The larvae of Spodoptera frugiperda have a large appetite and miscellaneous feeding habits, and can damage 353 species of host plants in 76 families. Since Spodoptera frugiperda invaded Jiangcheng County, Yunnan Province, my country in January 2019, it h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 金涛林玉英马光昌温海波刘俊彬
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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