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Large-scale raising method of dominant parasitoid wasps of liriomyza sativae

A technology of Liriomyza sativus and parasitic wasps, which is applied in the field of large-scale breeding of dominant parasitic wasps of Liriomyza sativae, can solve the problems of not being able to meet the pest control work in the field, not suitable for large-scale breeding, and the population is not easy to maintain species, etc., to achieve The feeding method is simple and easy, meets the needs of experimental research, and reduces the effect of economic loss

Inactive Publication Date: 2012-07-04
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is time-consuming, labor-intensive, low-yield, high-cost, and the population is not easy to preserve species. It is not suitable for large-scale breeding and cannot meet the actual field pest control work.
In foreign countries, Adenophora has been commercialized, but its cost is relatively high. The price of Adenophora sold in European and American markets is 0.065-0.349 US dollars each. According to the current calculation of 1 US dollar to 6.3 yuan, one is about 0.41 to 2.2 yuan, there is no report of commercialization of Fuxinji Xiaofeng

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Artificial large-scale rearing of the dominant parasitic wasps Liriomyza spp. and A. fuxini. The basic facilities and methods for raising are as follows:

[0025] (1) Basic facilities

[0026] Nursery room or insect breeding room: about 12m each 2(4.4×2.7m), the roof is transparent glass, 30-40cm away from the glass is a shading net, which can reduce the indoor temperature in summer, and a 2-hp heating and cooling air conditioner is installed in the room to control the indoor temperature of the nursery or insect breeding room; Install a microcomputer time-controlled switch to automatically control the switching time of all lamps in the nursery room or insect breeding room; put the insect cages in the nursery room.

[0027] Insect cage: use universal angle steel to make a cage with a length × width × height of 100 × 70 × 60cm, use 100-mesh glass yarn to cover it, and make a door with a zipper on the side of 100 × 60cm, for replacement For host plants.

[0028] Insect ...

Embodiment 2

[0042] Put 100 pairs, 200 pairs, and 300 pairs of Liriomyza sativae adults into the insect cages respectively, put 15 green bean plants with two true leaves fully unfolded in the insect cages, and plant 3 green beans in every pot. After 24 hours, the green beans were removed and put into the insect culture room. After 3 days, check the parasitic situation of Liriomyza sativae on the leaves of the plants, and check the pupation stage of Liriomyza sativae after 7 days.

[0043] Under the light conditions of 27°C and L:D=14:10, under the condition of certain leaves of the host plant, the number of eggs laid under the density of Liriomyza sativae in the inoculation cage, that is, the number of larvae at different ages, was compared, and the pupation number of mature larvae (Table 1). When the density of Liriomyza sativae was 200 pairs, its mature larvae and pupae number were the best, and when it was 300 pairs, because of the excessive amount of insects on the leaves of the host p...

Embodiment 3

[0047] Under the conditions of 27℃ and L:D=14:10 light, and under the condition of certain host larvae, the parasitoids with different densities released in the pitcher cage were compared to study the maximum bee production.

[0048] Insert 60 pairs, 80 pairs, 100 pairs, 120 pairs, and 140 pairs of 48-hour eclosion into the insect cages, and put 18 plastic cups in each insect cage. , each cup is inserted with 5 green bean plants with an average of 30 2nd instar Liriomyza sativae larvae parasitized on each leaf, feeding and laying eggs for the parasitic wasps, while hanging 2 with cotton thread in each receiving cage A cotton ball containing 10% honey water is used to supplement nutrition and moisture for the parasitic bees. The humidity of the cotton balls is just enough to prevent water from dripping out; remove the parasitic bees and the cotton balls stained with honey water after 48 hours; pick up the bees On the 4th day afterward, check the parasitism rate of the parasitic...

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Abstract

The invention discloses a large-scale raising method of dominant parasitoid wasps of liriomyza sativae. The method comprises the following steps of: (1) cultivating host plants; (2) reproducing liriomyza sativae; and (3) raising parasitoid wasps. The method has the following beneficial effects that: the method is simple and practical; the cost is lower; the dominant parasitoid wasps of liriomyza sativae can be raised on a large scale; and huge economic, social and ecological benefits can be brought by releasing the dominant parasitoid wasps in the areas seriously harmed by liriomyza sativae.

Description

technical field [0001] The invention relates to an artificial breeding method for parasitic wasps, in particular to a large-scale breeding method for dominant parasitic wasps of Liriomyza sativae. Background technique [0002] Liriomyzae refers to the genus Liriomyzae of the family Agromyzidae and the subfamily Phytomyzinae of the order Diptera ( Liriomyza Mik) fly insects. So far, 2,517 species of Lepidomydae have been reported, of which more than 338 species of Liriomyza have been recorded, mainly distributed in temperate regions of the world, and only a small number of species are distributed in the tropics. The vast majority of species in this genus feed on host plants in the form of leaf miners, 23 of which have important economic significance, mainly Liriomyza sativae ( Liriomyza. bryoniae ), Liriomyza sativae ( L. sativae ), Liriomyza sativae ( L. huidobrensis ) and Liriomyza clover ( L. trifolii ) and other 10 species, whose host range can reach hundreds of...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 王伟刘万学程立生万方浩王文霞
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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