The invention relates to a generating device of quasi-monodispersed submicron aerosol by using an evaporation condensation method. The generating device comprises a high-pressure gas source, a first filter, a sprayer, a diffusion desiccator, a second filter, a saturator, a reheater, a condenser tube, and a first valve as well as a second valve, wherein, the first valve and the second valve are respectively used for adjusting the flow capacity of the second filter and the saturator; the high-pressure gas source adopts a high-pressure air source; the reheater and the condenser tube adopt metallic conduits; a baffle plate is arranged at a jet exit of the sprayer; a screen mesh or a closing ring is arranged at the inlet of the condenser tube. The generating device is convenient and durable, the running cost is low, the median diameter in aerosol generating counting ranges from 0.08 micrometres to 0.5 micrometres, the geometric standard deviation is less than 1.3, the counting concentration ranges from 1*10<4> to 1*10<8>/cm<3>, the stability and the repeatability are good when in generation, and the requirements of various equipment inspections and experimental researches can be satisfied.