40results about How to "Meet the needs of experimental research" patented technology

The invention relates to a generating device of quasi-monodispersed submicron aerosol by using an evaporation condensation method. The generating device comprises a high-pressure gas source, a first filter, a sprayer, a diffusion desiccator, a second filter, a saturator, a reheater, a condenser tube, and a first valve as well as a second valve, wherein, the first valve and the second valve are respectively used for adjusting the flow capacity of the second filter and the saturator; the high-pressure gas source adopts a high-pressure air source; the reheater and the condenser tube adopt metallic conduits; a baffle plate is arranged at a jet exit of the sprayer; a screen mesh or a closing ring is arranged at the inlet of the condenser tube. The generating device is convenient and durable, the running cost is low, the median diameter in aerosol generating counting ranges from 0.08 micrometres to 0.5 micrometres, the geometric standard deviation is less than 1.3, the counting concentration ranges from 1*10<4> to 1*10<8> / cm<3>, the stability and the repeatability are good when in generation, and the requirements of various equipment inspections and experimental researches can be satisfied.

The invention relates to a 1600kV / 50mA outdoor mobile direct current voltage generator which comprises a direct current voltage generator body, a charging voltage regulation device and a computer measuring and controlling system; the direct current voltage generator body adopts a five-stage voltage doubling rectifying circuit with symmetrical single sides, the complete set of the test units adopts a tower type combined structure and a container transportation method as well as the modular design. A direct-current voltage generator body module, the charging voltage regulation device, a computer measuring and controlling system operating platform are respectively provided with hoisting devices, and the hoisting devices can be moved on a transport vehicle; the invention utilizes the 50Hz power frequency power supply as the rectifier source to obtain the direct-current voltage, the quality of the direct-current power supply is good, the capacity is large, and the demands of experimental investigation of the extra-high voltage direct current transmission project can be met; and due to the adoption of the modular design, the rapid assembly and disassembly can be realized on site, and the polarity can be changed according to the complete set of the test units with required testing voltage.

The invention relates to a GIS equipment fault simulation test system which is characterized by comprising a piece of single-phase complete interval GIS equipment, a complete current boosting apparatus and a complete voltage boosting apparatus. Power, after voltage regulation by a voltage regulator, is output through a gas test transformer. The current boosting apparatus is sleeved in a GIS pipe female housing so as to achieve an aim of current boosting. In such work condition, the operation work condition is simulated through the voltage boosting apparatus and the current boosting apparatus, stimulants such as tip ends and particles are built in a GIS bus test segment for charged detection test study. On the basis of the test study, an effective detection means of GIS equipment internal typical faults and corresponding determination bases are proposed. The GIS equipment fault simulation test system can be used for performing fault recurrence and charged detection work under rated operation voltage and operation current, and also be used for performing insulation characteristic test study, so as to realize validation research on the field insulation detection effective means.

The invention provides dynamic simulation device and method thereof of a super and extra high voltage controllable magnetic control shunt reactor, wherein the dynamic simulation device is capable of accurately simulating magnetic control shunt reactors with different capacities in 500kV, 750kV and 1,000kV power transmission systems. A simulation body consists of three single-phase magnetic control reactors; each single-phase magnetic control reactor is in a three-column structure; two beside core columns are working iron cores; a main alternating current winding and a direct current control coil are wound on each working iron core; and main alternating current windings on the two iron core columns are connected in parallel and connected to a power grid. Control windings are separated from the main windings so as to ensure the safety and the reliability of the device during work. The main windings are provided with twelve turn-to-turn short circuit taps which can respectively simulate 1-25 percent of turn-to-turn short circuit faults from the high-voltage side and the neutral point side; and the control windings are provided with seven turn-to-turn short circuit taps which can simulate 1-25 percent of turn-to-turn short circuit faults from the neutral point side. The body of the device is provided with a current transformer and a voltage transformer on a primary side, which are used for meeting the requirement for protecting a reactor body in a dynamic simulation test.

Thee invention relates to the field of biological control, in particular to an artificial mass raising method for agasicles hygrophila. The non-winter artificial mass raising method for the agasicles hygrophila includes the following steps: (1) alternanthera philoxeroides is grown in a greenhouse; (2) when the alternanthera philoxeroides plants are as high as 15cm to 20cm and the stem diameter reaches more than 2.6cm, the agasicles hygrophila is evenly released into the greenhouse according to the density of 10 to 20 adults per square meter; (3) spray irrigation is carried out for one to two times each day, 15 to 20 minutes each time; (4) every 5 to 7 days, the density of the agasicles hygrophila in the greenhouse is surveyed, and dynamic population monitoring is carried out; (5) after the agasicles hygrophila is released for 40 to 60 days, when the number of the adults is notably increased, the adults are collected and stored. The artificial mass raising technique for the agasicles hygrophila is simple, and is easy to master, the raising cost is low, and after being released into the field to control the alternanthera philoxeroides, the agasicles hygrophila can bring enormous economic, social and ecological benefits.

The invention discloses a bayberry orchard Drosophila elanogaster artificial breeding method, and a method for breeding parasitic wasp with the same, belonging to artificial breeding technology of natural enemy insects. The method successively propagates Pachycrepoideus vindemmiae capable of controlling bayberry orchard Drosophila elanogaster through the steps of Drosophila elanogaster breeding to obtain pupae, egg laying of Pachycrepoideus vindemmiae on the pupae, development and eclosion of parasitic wasp, and adult wasp breeding. The method is simple and convenient, and is especially suitable for large-scale indoor breeding of Pachycrepoideus vindemmiae to give large amount of wasps for test or release in bayberry orchard. Non-professional breeding personnel can grasp operation skill in short time. The breeding method has low land occupation and cost.

The invention relates to the technical field of artificial environment simulation, and particularly relates to a programmable precision artificial climate box. The artificial climate box comprises a box body, and the box body is of a multi-layer structure and comprises an experiment layer, an interlayer and an equipment layer from top to bottom in sequence, wherein a separation plate is arranged between the experiment layer and the interlayer, and the interlayer is of a double-channel structure and comprises a first channel and a second channel; the artificial climate box also comprises a simulation system, wherein the simulation system is arranged in the interlayer and the equipment layer and is used for adjusting the internal environment of the artificial climate box; and the artificialclimate box further comprises a program control system, wherein the program control system is arranged in the equipment layer and is used for detecting environmental data in the artificial climate boxand controlling the simulation system. The double-channel structure is adopted in the artificial climate box, so that the problem of temperature and humidity fluctuation caused by low-temperature low-dew-point continuous operation is effectively solved, and the temperature and humidity control precision of the climate box can be effectively improved. Through the match of the program control system, the advantages of energy conservation, economical practicability, wide range and high precision can be achieved.

The invention provides a jet flow supporting and testing device for turning long and thin rods. The jet flow supporting and testing device comprises a jet device, a cutting force measuring device, a fixture device and the like. The jet device is formed by a handcart, a motor, a high-pressure pump, a water inlet pipe, a water outlet pipe, a spray gun, and the like. The cutting force measuring device is mainly formed by a universal dynamometer, an amplifier, a computer and the like. The fixture device is formed by a clamping piece, a clamping block, a rod piece, a connecting block, a supporting plate, a base plate and the like. The jet flow supporting and testing device can be used for carrying out jet flow basic experiments, carrying out jet-flow or non-jet-flow supporting contrast experiments on turning of the long and thin rods, studying the probability and practical application effects that jet flow is used as auxiliary supporting for turning of the long and thin rods.

The invention discloses a flow field visual measurement experimental device based on matched index of refraction. The device comprises a main loop system, a first cooling loop system, a second coolingloop system and a bypass branch. The bypass branch divides the main loop system into a first part and a second part, the first cooling system is located at the first part, the second cooling loop system is located at the second part, and the first part includes a liquid tank, a conventional centrifugal main pump and a main heat exchanger, the first cooling system also includes a main heat exchanger, the first cooling system takes away the heat generated by working of the conventional centrifugal main pump on fluid through the main heat exchanger so as to control the temperature of the main loop system, the second part includes an electromagnetic flowmeter and a test section, the second cooling loop system is set between the electromagnetic flowmeter and the test section, and the second cooling loop system is set for controlling the fluid temperature of the test section, and the bypass branch regulates the flow passing through the test section.

The invention belongs to the technical field of animal models of diabetes mellitus, and particularly relates to a modeling method for an animal model of type 2 diabetes mellitus. The method comprisesthe step that 20-100 lumens and 8-10 hours of illumination is applied during nighttime animal sleep for induction, wherein the induction time is 2-6 month. The method provided by the invention fills the blank of modeling of the animal model of the type 2 diabetes mellitus by a natural induction method, shortens the modeling time of the animal model, and has fewer side effects; and the problems such as too serious obesity and too high mortality of individuals do not easily occur, and feeding costs are greatly reduced.

The invention belongs to the technical field of impeller machinery, and relates to a plane cascade experimental piece structure, in particular to a cantilever clearance-variable plane cascade experimental device. According to the device, a movable blade fixing plate and a movable blade fixing end plate are installed on a cascade side plate left grid plate, so that a novel clearance-variable planecascade experiment grid plate structure is formed, a single-side cantilever blade can be fixed, moreover, the position between the blade fixing plate and the left grid plate can be adjusted through common adjustment of a fixing bolt and a jacking bolt, then the change of the size of a clearance can be carried out, the clearance adjustment of a cantilever blade in the plane cascade is realized, theexperiment research requirements of the blade top clearance change and the blade top modification of a gas compressor can be completely met, and the important significance is achieved for the performance research of the gas compressor.

The invention discloses an artificial massive rapid propagation technical method for brownbanded encarsia formosa which sequentially comprises the steps of nursery stock purchasing, culture solution preparation, leaf cuttage and water culture, aphid inoculation, aphid culture, bee breeding in a bee breeding room, chalcidoid propagation, stiff aphid collection and adult bee collection, wherein parasitic wasp is encarsia formosa. According to the invention, about 20 days are needed from nursery stock purchasing to harvesting of adult chalcidoid, 200-220 thousand chalcidoids can be bred, and thechalcidoid can also be bred in batches according to a plan. According to the breeding method disclosed by the invention, a massive insect source can be provided for provinces or cities with severe aphid damage, so as to relieve the harm of aphid to crops and lower economic loss. The invention not only provides a rapid propagation method of brownbanded encarsia formosa, but also provides a collecting method of brownbanded encarsia formosa stiff aphid and adult bees, and provides a complete production process for commercialized production of parasitic wasp brownbanded encarsia formosa for the first time, and thus providing a massive natural enemy resource for biological control of aphid.

The invention belongs to fluidized bed related equipment and in particular relates to a device for measuring concentration distribution of a single-element or multi-element particle system in a fluidized bed. The device is characterized in that multiple layers of side wall discharging holes are uniformly distributed on side walls on two sides of a main fluidized bed; guide rail chutes are respectively formed on an inner wall of the main fluidized bed at each side wall discharging hole, and material-taking separation plates are respectively arranged on the guide rail chutes; a gas pre-distribution chamber is separated on the lower part of the main fluidized bed through a gas distribution plate; the bottom of the main fluidized bed is provided with a bottom distribution plate; annular space air inlet pipes are arranged in the gas pre-distribution chamber and on the bottom distribution plate; a jet air inlet nozzle is arranged on the gas distribution plate; and one end of a jet air inlet pipe is connected with the jet air inlet nozzle, and the other end of the jet air inlet pipe extends out of the bottom distribution plate. By adoption of gradual stratification sampling in a static state, particles on each layer can be totally taken, an error is avoided, and the actual distribution conditions of the particles can be really reflected; and moreover, the device is wide in application range and can be used for researching particle distribution under different operating conditions.

The invention discloses a plasmid transforming method and relates to the fields of molecular biology and genetic engineering. The plasmid transforming method comprises the steps as follows: target exogenous plasmid and a competence host cell with an ygiZ gene knocked out are mixed. The ygiZ gene of the host cell is knocked out, so that the transforming rate is increased. The method has the characteristic of high transforming efficiency for large plasmid. Compared with wild type host cells, the host cell with the ygiZ gene knocked out has the transforming efficiency improved by 2.05 times for plasmid pUC19, 2.60 times for pET-32a and 1.67 times for plasmid p1304. The method greatly improves the plasmid transforming efficiency and has very great significance in the molecular biology field and the genetic engineering field.

The invention relates to a natural enemy insect rapid propagation technology, and particularly relates to the method for propagating haematopus aphid wasps rapidly by using temperature transition. The method comprises the steps of cultivating a host plant cotton; feeding haematopus aphid wasps with bemisia tabaci, wherein the best temperature for the growth of the haematopus aphid wasps imago at an early stage is 30 DEG C; and the oviposition temperature at the imago stage is 26 DEG C; the darkness suitable for lighting L:D=14:10; and the suitable humidity is 60-80%. The artificial haematopus aphid wasps breeding method is used to accelerate the parasitic wasps' breeding rate while raising breeding temperature without reducing the parasitic wasps' breeding efficiency, therefore, the method is especially convenient for breeding the parasitic wasps indoor in large scale, and the wasps can be propagated rapidly.

The invention relates to a heat insulation experiment bin and a thermal runaway experiment method.The heat insulation experiment bin comprises a bin body, a heating device, at least one first temperature sensor and at least one second temperature sensor, the bin body comprises an inner shell and an outer shell, and an interlayer cavity is formed between the outer shell and the inner shell; the inner shell is provided with an experiment inner cavity, the experiment inner cavity is configured to be used for placing a target object, the bin body is provided with a bin opening communicated with the experiment inner cavity, the bin opening is provided with a bin door, a heating device is arranged in the interlayer cavity, at least one first temperature sensor is arranged in the experiment inner cavity, and a second temperature sensor is arranged in the experiment inner cavity. The first temperature sensor is configured to be used for acquiring temperature information of a target object, the at least one second temperature sensor is arranged in the experiment inner cavity, and the second temperature sensor is configured to be used for acquiring environment temperature information of the experiment inner cavity. Therefore, the thermal runaway scene can be effectively simulated, and the existing experimental research requirements are met.

The invention relates to a method for improving the parasitic rate of parasitic wasps to whitefly MED cryptic species, the method comprising the following steps: (1) cultivation of host plants; (2) breeding of host whitefly nymphs; (3) parasitic wasps (4) Cultivation, preservation and training of bank plants before release; (5) Field release of bank plants. The invention provides a method for improving the parasitic efficiency of the bemisia tabaci after the parasitoid bees are released from bank plants outdoors, establishes a stable field population, and satisfies the needs of efficiently controlling the bemisia tabaci of field crops.

The invention provides dynamic simulation device and method thereof of a super and extra high voltage controllable magnetic control shunt reactor, wherein the dynamic simulation device is capable of accurately simulating magnetic control shunt reactors with different capacities in 500kV, 750kV and 1,000kV power transmission systems. A simulation body consists of three single-phase magnetic control reactors; each single-phase magnetic control reactor is in a three-column structure; two beside core columns are working iron cores; a main alternating current winding and a direct current control coil are wound on each working iron core; and main alternating current windings on the two iron core columns are connected in parallel and connected to a power grid. Control windings are separated fromthe main windings so as to ensure the safety and the reliability of the device during work. The main windings are provided with twelve turn-to-turn short circuit taps which can respectively simulate 1-25 percent of turn-to-turn short circuit faults from the high-voltage side and the neutral point side; and the control windings are provided with seven turn-to-turn short circuit taps which can simulate 1-25 percent of turn-to-turn short circuit faults from the neutral point side. The body of the device is provided with a current transformer and a voltage transformer on a primary side, which areused for meeting the requirement for protecting a reactor body in a dynamic simulation test.

The invention relates to the field of insect breeding, and specifically discloses an indoor large-scale breeding method for Liriomyza longina, comprising the following steps: placing the eclosion Liriomyza longina in a beekeeping cage; Put the inoculation boxes of instar Bactrocera dorsalis larvae and larval feed into the beekeeping cages for inoculation; continue to raise the Bactrocera dorsalis larvae in the replaced inoculation boxes, and move them to the pupation box when the larvae develop to the age of 7 days Let the larvae pupate in the middle, and collect the remaining unemerged pupae within 10 to 14 days of pupation, which is the parasitic pupa; place the parasitic pupae in the beekeeping cage until the Liriomyza spp. eclosion emerges, and then the cocoon of Liriomyza spp. bee. The indoor large-scale breeding method of Liriomyza longina of the present invention is simple and easy, can be operated indoors, and can reproduce Liriomyza longtail on a large scale, realizes uninterrupted reproduction of the Liriomyza long-tailed throughout the year, and can be planted to melons and fruits The area provides a large number of insect sources for the control of Bactrocera dorsalis, which is harmful to melon and fruit crops.

The invention relates to methods of obtaining mesenchymal stem cells and is to provide a method of high-effectively obtaining mesenchymal stem cells from compact bone of mice. The method of high-effectively obtaining mesenchymal stem cells of mice includes the steps of: separating mesenchymal stem cells of mice, performing primary culture, and performing amplification culture to the mesenchymal stem cells of mice. The method greatly reduces the time of cell culture and can greatly increase quantity of produced cells, and is less in pollution of non-MSCs adherent cells. The invention, for the first time, discloses a multi-time bone sheet adhesion culture method, which can increase the yield of cells by 3-5 times. Through systematic researching on biological characteristics, such as growth status, surface markers, multidirectional differentiative potential and the like, of the MSCs from the compact bone of mice, it is proved that after 10 times of amplification culture in vitro, the cells still have strong characters of stem cells and can satisfy demands in experiment and researching. The method can quickly and high-effectively produce the stem cells. The obtained stem cells has high proliferation and growth speed and high differentiative potential.

The invention discloses a marine leakage type natural gas hydrate reservoir forming simulation experiment device and method based on a geotechnical centrifuge. The experiment device comprises a geotechnical centrifuge, a hanging basket assembly and a rotary arm carrying equipment assembly. The hanging basket assembly is hinged to one end of a geotechnical centrifuge rotary arm through a hanging basket connecting shaft and comprises a natural gas hydrate-containing soil body sample, a fixing component of the natural gas hydrate-containing soil body sample, a high-pressure bin, a water bath jacket and the like, and the rotary arm carrying equipment assembly is fixedly arranged on the geotechnical centrifuge rotary arm and used for achieving hydrate reservoir forming temperature control, gassource supply and data acquisition. According to the device and the method, a gravity field is simulated by centrifugal acceleration generated by high-speed rotation of a centrifugal machine in a laboratory to increase buoyancy; and on the premise that the gas flux is not changed, rapid and efficient simulation of the natural gas hydrate reservoir forming process under the buoyancy-controlled gasleakage condition is achieved, the limitation of an existing gas micro-flux supply technology is skillfully avoided, and the leakage type natural gas hydrate reservoir forming process is closer to theactual situation of a natural field.

The invention discloses an electronic cigarette atomization core tobacco juice consumption rate test system, which comprises a ten-thousand-minute balance used for weighing the weight of an atomization core and tobacco juice; the atomizing core fixing device is used for accommodating an atomizing core and cigarette liquid; the gas generating device is communicated with the atomizing core fixing device through a pneumatic pipe and is used for introducing suction airflow into the atomizing core fixing device; the flow meter is arranged on the pneumatic pipe and is used for reading the flow of gas introduced into the atomizing core fixing device by the gas generating device; the power supply is connected with the atomizing core through a wire and is used for supplying power to an electrified electrode of the atomizing core; and the timing stopwatch is used for calculating the power-on suction time. The invention further discloses a method for testing the consumption rate of the cigarette liquid of the atomization core of the electronic cigarette. The method has the beneficial effects that the consumption rate of the cigarette liquid in the atomization core can be simply, conveniently and quickly tested with relatively high precision.

The invention discloses a multi-field coupled in-vitro cell experimental device and a method. The device includes a container internally equipped with a thermal insulation interlayer and a magnetic stirrer, a flange cover provided with a plurality of holes on the surface, and a multifunctional sample stand. The upper end and the lower end of the container are respectively provided with a water inlet and a water outlet. The water outlet is provided with a ball valve. The magnetic stirrer is used for balancing the water bath temperature. The flange cover is connected to the top of the container to form a structure able to open or close the container. A porous cell culture plate-carrying electromagnetic field generation device and / or a single porous cell culture plate can be placed on the sample stand, which is placed in the container during experiment. The method comprises: putting the porous cell culture plate-carrying electromagnetic field generation device on the sample stand, then placing the sample stand into the container, and controlling the atmosphere, temperature and humidity conditions required by cell culture. The multi-field coupled in-vitro cell experimental device provided in the invention has the advantages of simple structure, low cost, convenient operation and multiple functions, and can meet the need of performing multiple groups of experiments at the same time.

The invention discloses a decapsulated egg drying device. The device comprises a tank body, a stirring device and an air blower, wherein the air blower is detachably connected to the bottom of the tank body through an elbow connector, the stirring device is arranged in the tank body, and a filter net is arranged between the bottom of the tank body and the elbow connector; the lower middle portion of the side face of the tank body is provided with a sampling port and a cover body opened and closed through a buckle, and a rotary handle is connected to the upper end of the stirring device in a bendable mode. The device has the advantages of being simple in structure, convenient to use, and capable of meeting the requirements of general experimental studies in a laboratory, controlling the humidity of a small number of worm eggs and facilitating scientific research.

The invention discloses a culture medium for raising sporulation quantity of Athelia bombacina and a culture method thereof. The formula comprises, by weight, 10-20 parts of oat, 15-20 parts of agar,10-15 parts of glucose, 0.5-1.0 part of CaCO3, 1-5 parts of NaCL and 1000 parts of water. The invention also provides a culture method of the culture medium for raising sporulation quantity of Atheliabombacina. By the use of the culture medium and under guidance of the culture method, Athelia bombacina can produce lots of spores within 5-7 days, and a layer of sporophore is formed on the surfaceof mycelia. The invention has advantages of short sporulation time, large yield of spores, high purity of spores and lasting pathogenicity. Requirements of various experimental studies can be fully met. Time can be greatly saved for researchers.

The invention relates to a method for obtaining pig neural crest stem cells. The method comprises the following steps: taking pig neck adipose tissues and chopping in a culture solution; standing andculturing; separating to obtain primary pig neural crest stem cells; carrying out steps including primary culture, amplification culture of the pig neural crest stem cells and the like to obtain the pig neural crest stem cells. A lot of tests and optimization are carried out to establish a set of effective pig neural crest stem cell separation and purification method; the method provided by the invention has the advantages of simple stem cell separation operation, good repeatability and high survival rate; after the cells are subjected to in-vitro amplification culture for 10 times, the cellsstill have relatively strong stem cell properties, can meet the requirements of experiment researches and are easy to culture; the neural crest stem cells can be rapidly and efficiently obtained, andthe obtained stem cells have a relatively rapid proliferation growth speed and a relatively strong differentiation potential. The speed and efficiency of developing nerves and repairing damaged nervous tissues are improved when the stem cells are used for treating. The method is successfully implemented so that a firm foundation is laid for clinical popularization and application of the neural crest stem cells and the neural crest stem cells can be possibly used as a neural crest stem cell medicine for development and research.

A bacterium for degrading dicoumaric acid glucoside and its breeding method and application: including step 1, extracting bacteria from Eupatoria adenophorum invasion plots, culturing and purifying them, and step 2, carrying out molecular identification of isolated bacterial single colonies; Step 3. Degradation test of dicoumaric acid glucoside. This bacterium has a strong effect of degrading dicoumaric acid glucoside. When applied to the soil invaded by Eupatorium adenophorum, it can greatly alleviate the damage caused by Eupatorium adenophorum. The allelopathy of native plants can promote the recovery of native plants in Eupatorium adenophorum invasion.

The invention relates to a 1600kV / 50mA outdoor mobile direct current voltage generator which comprises a direct current voltage generator body, a charging voltage regulation device and a computer measuring and controlling system; the direct current voltage generator body adopts a five-stage voltage doubling rectifying circuit with symmetrical single sides, the complete set of the test units adopts a tower type combined structure and a container transportation method as well as the modular design. A direct-current voltage generator body module, the charging voltage regulation device, a computer measuring and controlling system operating platform are respectively provided with hoisting devices, and the hoisting devices can be moved on a transport vehicle; the invention utilizes the 50Hz power frequency power supply as the rectifier source to obtain the direct-current voltage, the quality of the direct-current power supply is good, the capacity is large, and the demands of experimental investigation of the extra-high voltage direct current transmission project can be met; and due to the adoption of the modular design, the rapid assembly and disassembly can be realized on site, and the polarity can be changed according to the complete set of the test units with required testing voltage.