A kind of bacteria degrading dicoumaric acid glucoside and its breeding method and application

A technology of glucoside and dicoumaric acid, which is applied in the field of bacteria, can solve problems such as recovery difficulties, and achieve the effects of simple method, beneficial ecological recovery, and huge economic benefits

Inactive Publication Date: 2019-06-25
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the ultimate goal of management of invasive organisms is to restore native species where the organism has invaded, sometimes in habitats invaded by invasive plants such as Eupatorium adenophorum even if Eupatoria adenophorum is controlled or eliminated It is very difficult to restore, because the allelochemicals produced by the invaders are still in the soil, and the litter produced by the invaders will often release allelochemicals

Method used

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  • A kind of bacteria degrading dicoumaric acid glucoside and its breeding method and application
  • A kind of bacteria degrading dicoumaric acid glucoside and its breeding method and application
  • A kind of bacteria degrading dicoumaric acid glucoside and its breeding method and application

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Effect test

experiment example

[0131] Alleviating effect of Bacillus thuringiensis on p-dicoumarin glucoside:

[0132] In 2012, the seeds of three different plants were treated with dicoumaric acid glucoside at a concentration of 0.03mmol / L, and germination experiments were carried out. The control was performed without adding Bacillus thuringiensis, and the treatment was compared with adding Bacillus thuringiensis. In experiments, it was found that the addition of Bacillus thuringiensis can greatly alleviate the inhibitory effect of dicoumaric acid glucoside on plant seed germination and seedling growth. See Table 1 for specific results.

[0133] Table 1 Relief effect of Bacillus thuringiensis on dicoumaric acid glucoside (" / ", no obvious effect)

[0134]

[0135]

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Abstract

A bacterium for degrading dicoumaric acid glucoside and its breeding method and application: including step 1, extracting bacteria from Eupatoria adenophorum invasion plots, culturing and purifying them, and step 2, carrying out molecular identification of isolated bacterial single colonies; Step 3. Degradation test of dicoumaric acid glucoside. This bacterium has a strong effect of degrading dicoumaric acid glucoside. When applied to the soil invaded by Eupatorium adenophorum, it can greatly alleviate the damage caused by Eupatorium adenophorum. The allelopathy of native plants can promote the recovery of native plants in Eupatorium adenophorum invasion.

Description

technical field [0001] The invention relates to the field of screening and identification of bacteria capable of degrading dicoumaric acid glucoside, an allelochemical of the invasive weed Eupatorium adenophorum, in particular to a bacterium for degrading dicoumaric acid glucoside and a breeding method thereof and application. Background technique [0002] Eupatorium adenophora (scientific name: Ageratina adenophora Sprengel or Eupatorium adenophorum), also known as Liberation Grass, Destruction Grass, Red Deer Grass, Big Black Grass, Flower Cohosh, Fine Cohosh, is a perennial of the Asteraceae Eupatoria family. The herbaceous plant or subshrub, native to Mexico and Costa Rica, has become a global invasive species due to its strong reproductive ability since it was introduced as an ornamental plant around the world in the 19th century. In 2003, it ranked first in the "China's First Batch of Invasive Alien Species List" issued by the State Environmental Protection Administra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/689C12Q1/04A01N63/00A01P21/00C12R1/07
CPCA01N63/00C12N1/02C12N1/20C12Q1/689C12N1/205C12R2001/075
Inventor 吴爱平张欣洁段晓迪钟文刘悦
Owner HUNAN AGRICULTURAL UNIV
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