Multi-field coupling in vitro cell experiment device and method

Abstract

The invention discloses a multi-field coupled in-vitro cell experimental device and a method. The device includes a container internally equipped with a thermal insulation interlayer and a magnetic stirrer, a flange cover provided with a plurality of holes on the surface, and a multifunctional sample stand. The upper end and the lower end of the container are respectively provided with a water inlet and a water outlet. The water outlet is provided with a ball valve. The magnetic stirrer is used for balancing the water bath temperature. The flange cover is connected to the top of the container to form a structure able to open or close the container. A porous cell culture plate-carrying electromagnetic field generation device and / or a single porous cell culture plate can be placed on the sample stand, which is placed in the container during experiment. The method comprises: putting the porous cell culture plate-carrying electromagnetic field generation device on the sample stand, then placing the sample stand into the container, and controlling the atmosphere, temperature and humidity conditions required by cell culture. The multi-field coupled in-vitro cell experimental device provided in the invention has the advantages of simple structure, low cost, convenient operation and multiple functions, and can meet the need of performing multiple groups of experiments at the same time.

Description

technical field [0001] The invention relates to a cell culture device and method, in particular to a multi-field coupling in vitro cell experiment device and method, belonging to the field of medical devices. Background technique [0002] Natural somatic cells are in a complex microenvironment of the body, and the stress and strain, bioelectric field, and biomagnetic field of the natural body affect the evolution of the structure, shape and function of body tissue growth. Since Fukada and Yasuda's discovery of the macroscopic electromechanical properties of bone in 1957 and Bassett's subsequent discovery that stress promotes bone remodeling may be due to the potential generated by stress in bone, which stimulates cell responses and induces physiological changes in cells. [0003] Clinical applications of biomechanics include: orthopedic stretching of limbs, compression and fixation of fractures, craniomandibular traction, and stretching of skin and soft tissues. In terms of...

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Problems solved by technology

In addition to constructing the set force, electricity, and magnetic field in the study of in vitro cell experiments, the experimental environment must be compatible with the conventional cell culture environment, otherwise it cannot accurately simulate the internal environment of biological organisms, resulting in the lack of scientific basis for the research results

[0005] At present, the experimental devices for applying force field, magnetic field and electric field to cells include four-point bending cell force device, stomatal pressurized cell culture device, in vitro cell static magnetic field loading device and cell electrophoresis device, etc. However, these devices and instruments have applications Single function, cumbersome operation, high price, inability to conduct cell culture independently, difficulty in carrying out multiple experiments at the same time, unfavorable for research on the reaction between biological materials and cells, which requires a large number of samples and other inconvenient factors

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