Culture medium for raising sporulation quantity of Athelia bombacina and culture method thereof

A culture method and culture medium technology, applied in the biological field, can solve the problems of economic loss during storage and affect the research process, etc., and achieve the effects of short output time, long-lasting pathogenicity, and time saving

Inactive Publication Date: 2018-01-16
FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, a new disease was discovered during the fruit storage period. The pathogen is Athelia bombacina, which caused a large economic loss during the storage period. During the study of the biological characteristics of the fungus, it was found that Athelia bombacina did not produce fruit under conventional fungal culture conditions. spores, this phenomenon seriously affected the later research process
After reviewing the literature, there is still a lack of research on the spore-forming culture conditions of the pathogenic bacteria of this disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium for raising sporulation quantity of Athelia bombacina and culture method thereof
  • Culture medium for raising sporulation quantity of Athelia bombacina and culture method thereof
  • Culture medium for raising sporulation quantity of Athelia bombacina and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0026] 1. Effects of different temperatures on colony growth and spore production

[0027] Using classic PDA as the base medium, put the mycelia of Athelia bombacina cultured in advance on the PDA medium with a puncher with a diameter of 8mm, and then place them at 5°C, 10°C, 15°C, and 20°C respectively. , 25°C, and 30°C constant temperature biochemical incubators, and measure the colony diameter on the 3d, 5d, 7d, and 9d of cultivation, respectively. Finally, it was found that the culture temperature suitable for the growth of the bacterial colony was 20-25°C, and the optimum culture temperature was 25°C, but no conidia were found during the culture process.

[0028] 2. Effects of different C sources on colony growth diameter and sporulation

[0029] According to the PDA medium formula, glucose was replaced with maltose, galactose, la...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a culture medium for raising sporulation quantity of Athelia bombacina and a culture method thereof. The formula comprises, by weight, 10-20 parts of oat, 15-20 parts of agar,10-15 parts of glucose, 0.5-1.0 part of CaCO3, 1-5 parts of NaCL and 1000 parts of water. The invention also provides a culture method of the culture medium for raising sporulation quantity of Atheliabombacina. By the use of the culture medium and under guidance of the culture method, Athelia bombacina can produce lots of spores within 5-7 days, and a layer of sporophore is formed on the surfaceof mycelia. The invention has advantages of short sporulation time, large yield of spores, high purity of spores and lasting pathogenicity. Requirements of various experimental studies can be fully met. Time can be greatly saved for researchers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a culture medium and a culture method for improving the spore production of Athelia bombacina. Background technique [0002] In recent years, a new disease was discovered during the fruit storage period. The pathogen is Athelia bombacina, which caused a large economic loss during the storage period. During the study of the biological characteristics of the fungus, it was found that Athelia bombacina did not produce fruit under conventional fungal culture conditions. spores, this phenomenon seriously affected the later research process. After reviewing the literature, there is still a lack of research on the spore-forming culture conditions of the pathogenic bacteria of this disease. Contents of the invention [0003] The object of the present invention is to provide a kind of medium that is beneficial to improving Athelia bombacina sporulation yield and culture method thereof, the c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12N1/14C12R1/645
Inventor 贾晓辉崔建潮孙平平王文辉
Owner FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap