Culture medium for raising sporulation quantity of Athelia bombacina and culture method thereof

Abstract

The invention discloses a culture medium for raising sporulation quantity of Athelia bombacina and a culture method thereof. The formula comprises, by weight, 10-20 parts of oat, 15-20 parts of agar,10-15 parts of glucose, 0.5-1.0 part of CaCO3, 1-5 parts of NaCL and 1000 parts of water. The invention also provides a culture method of the culture medium for raising sporulation quantity of Atheliabombacina. By the use of the culture medium and under guidance of the culture method, Athelia bombacina can produce lots of spores within 5-7 days, and a layer of sporophore is formed on the surfaceof mycelia. The invention has advantages of short sporulation time, large yield of spores, high purity of spores and lasting pathogenicity. Requirements of various experimental studies can be fully met. Time can be greatly saved for researchers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a culture medium and a culture method for improving the spore production of Athelia bombacina. Background technique [0002] In recent years, a new disease was discovered during the fruit storage period. The pathogen is Athelia bombacina, which caused a large economic loss during the storage period. During the study of the biological characteristics of the fungus, it was found that Athelia bombacina did not produce fruit under conventional fungal culture conditions. spores, this phenomenon seriously affected the later research process. After reviewing the literature, there is still a lack of research on the spore-forming culture conditions of the pathogenic bacteria of this disease. Contents of the invention [0003] The object of the present invention is to provide a kind of medium that is beneficial to improving Athelia bombacina sporulation yield and culture method thereof, the c...

AI Technical Summary

Problems solved by technology

[0002] In recent years, a new disease was discovered during the fruit storage period. The pathogen is Athelia bombacina, which caused a large economic loss during the storage period. During the study of the biological characteristics of the fungus, it was found that Athelia bombacina did not produce fruit under conventional fungal culture conditions. spores, this phenomenon seriously affected the later research process

After reviewing the literature, there is still a lack of research on the spore-forming culture conditions of the pathogenic bacteria of this disease

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