Large-scale production method of diaphorina aliensis

A technology of lice jumping wasps and production methods, which is applied in the direction of animal husbandry, etc., can solve the problems of no public reports of artificial propagation and release, no biological experiment reports, etc. The effect of high activity and uniform age of nymphs

Active Publication Date: 2021-10-29
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] With regard to the biological control of citrus psyllids by using Ali-eating lice and jumping bees, our country currently mainly stays at the level of...

Method used

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  • Large-scale production method of diaphorina aliensis
  • Large-scale production method of diaphorina aliensis

Examples

Experimental program
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Embodiment 1

[0056] In this embodiment, 1 breeding cage is set, and 1 Siji tangerine plant is placed in the cage. The pot and soil are brought when purchased and have not been replaced. Trim a branch growing from the base of the stem to one leaf and one bud, and cut off all the leaves of the other branches, leaving about 50 buds in total. The final plant is about 30cm wide and 85cm high.

[0057] When the shoot length is about 3 cm, insert 100 citrus psyllids, 50 male and 50 male. After 72 hours (3 days) the citrus psyllids are removed leaving only freshly laid eggs on the plants. The date of inoculation of citrus psyllids was recorded as the first day, and the development of citrus psyllid nymphs on the shoots was observed every day from the seventh day.

[0058] On the 12th day, the citrus psyllid nymphs had entered the third instar, and the instars were relatively consistent. According to estimates, the number of nymphs is about 150. According to female bees: citrus psyllid nymphs = ...

Embodiment 2

[0063] In this embodiment, 1 breeding cage is set, and 1 Siji tangerine plant is placed in the cage. The pot and soil are brought when purchased and have not been replaced. The whole plant is trimmed to one leaf and one bud, leaving about 50 buds, and the final plant is about 30cm wide and 85cm high.

[0064] When the shoot length is about 3 cm, insert 100 citrus psyllids, 50 male and 50 male. After 72 hours (3 days) the citrus psyllids are removed leaving only freshly laid eggs on the plants. The date of inoculation of citrus psyllids was recorded as the first day, and the development of citrus psyllid nymphs on the shoots was observed every day from the seventh day.

[0065] On the 11th day, most of the citrus psyllid nymphs have entered the second instar, and at this time, Ali-eating psyllid nymphs were inoculated. There are about 120 citrus psyllid nymphs, according to female bees: citrus psyllid nymphs = 1:30 (quantity ratio), 4 female psyllid bees were connected to Ali...

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Abstract

The invention relates to the field of biological prevention and control of agricultural pests, and discloses a large-scale production method of diaphorina algida. The large-scale production method of the diaphorina algida mainly comprises the following four steps of (1) establishing a diaphorina algida population; (2) preparing a diaphorina citri population to be parasitized; (3) inoculating a parasitic wasp of diaphorina citri, namely the diaphorina algida, into the diaphorina citri population to be parasitized; and (4) collecting the parasitic wasps. The large-scale production method of the diaphorina algida is simple in process and controllable in breeding scale, and propagation expanding work can be carried out all year round and is not limited by seasons; meanwhile, eclosion time of the parasitic wasps is concentrated, population quantity is controllable, interference of phylloxera does not need to be considered during wasp collection, and operation is simple; meanwhile, the method has no special rigid requirements on the types and sizes of host plants and is suitable for all the host plants of the diaphorina citri, and the utilization rate of the host plants is high.

Description

technical field [0001] The invention relates to the field of biological control of agricultural pests, in particular to a method for the large-scale production of Ali-eating lice and jumping bees. Background technique [0002] In the past ten years, the research reports on the citrus psyllid Diaphorina citri parasitoids in my country mainly focused on two species, Tamarixia radiata and Diaphorencyrtus aligarhensis. [0003] The earliest distribution record of A. ali-eating bee wasp was recorded in India by Shafee in 1975. Because it is the same parasitoid of citrus psyllids as the citrus psyllid, it was quickly used for the biological control of citrus psyllids. Domestic targeted introduction and utilization began around 1995 in Taiwan, China, and the relevant literature in mainland China is still dominated by reports on foreign research and records of sporadic distribution in China, but the number is increasing. [0004] Around 2000, U.S. researchers began to introduce th...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 孙子强王朝红高晶修宝林叶凤娴毛润乾
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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