In-vitro culture method of balantidium coli

Abstract

The invention discloses an in-vitro culture method of balantidium coli. The in-vitro culture method of balantidium coli comprises the following steps of (1) polypide inoculation: inoculating pollutant containing balantidium coli in an RSS culture medium and culturing for 48 h at a temperature of 28 DEG C; (2) preparation of the culture medium: preparing the culture medium according to 15-25% by volume of new-born calf serum and 75-85% by volume of a 199 culture medium with a pH value of 6.0-8.0, adding 20-40 mg of starch in every 3 mL of the culture medium; and (3) polypide culture: (a) adding 10,000 U/mL of penicillin and streptomycin in the culture medium formed by the step (2), (b) inoculating a balantidium coli suspension in the culture medium formed by the step (a), culturing for 96-108 h at the temperature of 28 DEG C to obtain balantidium coli having the highest population density being 42.75-74.5 times of an inoculation density. The in-vitro culture method of balantidium coli has the advantages of simple preparation method, convenient operations, relatively good stability and high population density.

Description

technical field [0001] The invention relates to an in vitro culture method of schizociliates colon, belonging to the technical fields of animal parasitology and protozoan in vitro culture. Background technique [0002] Balantidium coli is a protozoan parasitic in the intestinal tract and is currently known to be the largest protozoa infecting humans and non-human primates (Solaymani-Mohammadi S, Petri WA Jr. Zoonotic implications of swine-transmitted protozoal infections. Vet Parasitol 2006, 140:189–203). Balantidiosis is a worldwide distribution, mainly prevalent in tropical and subtropical regions. Human balanticiliasis is acquired through contaminated water sources, vector insects, sanitary conditions (including personal and environmental), and close contact with pigs. The infection rate of immunosuppressed patients such as AIDS patients and leukemia patients is higher than that of normal people; the infection rate of people in mental hospitals and shelters is higher th...

AI Technical Summary

Problems solved by technology

The culture media such as LE, Robinson's, TYSGM-9, Balamuth's, Jones's, etc. currently used for in vitro culture of schizociliates coli have problems such as complex preparation and poor stability (Clark, C.G., and L.S. Diamond. Methods for cultivation of luminal parasitic protists of clinical importance. Clin. Microbiol. Rev. 2002, 15:329–341.)

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