Cultivation method of all-male pseudosciaena crocea and matched preservation line

Abstract

The invention provides a breeding method of all-male pseudosciaena crocea and a matched preservation line, and relates to the technical field of pseudosciaena crocea breeding. According to the all-male larimichthys crocea, YY type male larimichthys crocea and XX type female larimichthys crocea are paired, and all produced offspring are normal XY type male larimichthys crocea. The cultivation method of the large yellow croaker matched preservation line comprises the following steps: firstly, performing induced transformation on male large yellow croaker by using estrogen to obtain XY type female large yellow croaker; then the XY type female pseudosciaena crocea subjected to sexual transformation is matched with normal XY type male pseudosciaena crocea, and produced offspring contains YY type male genetic pseudosciaena crocea; and pairing the YY type male genetic large yellow croaker with the normal XX type female large yellow croaker, wherein all the produced offspring are the normal XY type male large yellow croaker. Through the application of estrogen induction and genetic sex identification technologies, the sex control accuracy of the large yellow croaker is realized, and a new technical means is provided for the aquaculture industry.

Description

Technical Field

[0001] The invention relates to the technical field of large yellow croaker breeding, in particular to a breeding method of an all-male large yellow croaker and a matching preservation system. Background Art

[0002] Large yellow croaker is an important marine aquaculture fish in my country, but there are sexual dimorphism differences in growth rate, body size, etc. during the aquaculture process. All-male large yellow croaker has advantages in growth rate, feed utilization rate, etc., so the cultivation of all-male large yellow croaker is of great significance to the aquaculture industry.

[0003] In the prior art, the cultivation of all-male large yellow croaker has not been popularized, that is, it is impossible to obtain all-male large yellow croaker. Therefore, it is urgent to provide a fast, convenient and effective cultivation method that can screen and cultivate fast-growing all-male large yellow croaker varieties. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies in the prior art, the present invention provides a method for cultivating an all-male large yellow croaker and a matching preservation system, which solves the problems raised by the above-mentioned background technology.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical scheme: an all-male large yellow croaker, which is produced by pairing a YY-type male large yellow croaker with an XX-type female large yellow croaker, and all the offspring produced are normal XY-type male large yellow croaker.

[0008] A method for cultivating an all-male large yellow croaker supporting preservation system, based on the all-male large yellow croaker as described above, comprises the following steps:

[0009] Step 1: First, use estrogen to induce sexual transformation of male large yellow croaker to obtain XY type female large yellow croaker;

[0010] Step 2: Then pair the sex-transformed XY female yellow croaker with the normal XY male yellow croaker, and the offspring produced include YY male genetic yellow croaker;

[0011] Step 3: Pair the YY-type male yellow croaker with the normal XX-type female yellow croaker, and all the offspring produced will be normal XY-type male yellow croaker.

[0012] Preferably, in step 1, the estrogen is estradiol E2.

[0013] Preferably, in step 2, genetic sex PCR is used to identify and then screen to obtain YY type male genetic large yellow croaker.

[0014] A method for cultivating an all-male large yellow croaker supporting preservation system, based on the all-male large yellow croaker as described above, comprises the following steps:

[0015] Step 1: First, use estrogen to induce sexual transformation of male large yellow croaker to obtain XY type female large yellow croaker;

[0016] Step 2: Then, the sex-transformed XY female large yellow croaker is subjected to gynogenetic development, and the offspring produced include YY male genetic large yellow croaker;

[0017] Step 3: Pair the YY male yellow croaker homozygous line with the normal XX female yellow croaker, and all the offspring produced will be normal XY male yellow croaker.

[0018] Preferably, in step 1, the estrogen is estradiol E2.

[0019] Preferably, in step 2, genetic sex PCR is used to identify and then screen to obtain YY type male genetic large yellow croaker.

[0020] A method for cultivating an all-male large yellow croaker supporting preservation system, based on the all-male large yellow croaker as described above, comprises the following steps:

[0021] Step 1: First, use estrogen to induce sexual transformation of male large yellow croaker to obtain XY type female large yellow croaker;

[0022] Step 2: Then, the sex-transformed XY female large yellow croaker is subjected to gynogenetic development, and the offspring produced include YY male genetic large yellow croaker;

[0023] Step three: Induce feminization of the YY male large yellow croaker homozygous line to obtain the YY female large yellow croaker homozygous line, and use the YY female large yellow croaker homozygous line to construct a YY male homozygous line on a large scale.

[0024] Preferably, in step 1, the estrogen is estradiol E2.

[0025] Preferably, in step 2, genetic sex PCR is used to identify and then screen to obtain YY type male genetic large yellow croaker.

[0026] (III) Beneficial effects

[0027] The present invention provides a method for cultivating an all-male large yellow croaker and a matching preservation system, which has the following beneficial effects:

[0028] 1. Through specific sex control technology, the purity of male large yellow croaker in the breeding population is ensured, which helps to stabilize genetic traits and reduce the impact of sexual dimorphism.

[0029] 2. This method is easy to operate and can be easily promoted and used in aquaculture, which will help promote the large-scale production of all-male large yellow croaker.

[0030] 3. Through the application of estrogen induction and genetic sex identification technology, the accuracy of sex control of large yellow croaker is achieved, providing new technical means for the aquaculture industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a schematic diagram of the cultivation method of the present invention. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] The present invention establishes a three-line matching (three-line matching between the original line XX♀, the male homozygous line YY♂ and the male homozygous conversion line YY♀) all-male large yellow croaker breeding technology and production system based on sex control and sex control breeding technology. The technology of using sex hormones to induce the breeding of functional large yellow croaker females (XY♀, YY♀) has been established; the sex-specific molecular markers of large yellow croaker have been developed and optimized, which can effectively identify genetic females (XX), males (XY) and super males (YY).

[0034] Embodiment 1:

[0035] The embodiment of the present invention provides an all-male large yellow croaker, which is produced by pairing a YY-type male large yellow croaker with an XX-type female large yellow croaker, so that all the offspring are normal XY-type male large yellow croaker.

[0036] A method for cultivating an all-male large yellow croaker supporting preservation system, based on the above all-male large yellow croaker, comprises the following steps:

[0037] Step 1: First, the male large yellow croaker is induced to transform with estrogen, wherein the estrogen is estradiol E2, to obtain an XY type female large yellow croaker;

[0038] Step 2: Pairing the sex-transformed XY female large yellow croaker with the normal XY male large yellow croaker, and producing offspring including YY male genetic large yellow croaker. Specifically, genetic sex PCR is used to identify and then screen to obtain YY male genetic large yellow croaker;

[0039] Step 3: Pair the YY-type male yellow croaker with the normal XX-type female yellow croaker, and all the offspring produced will be normal XY-type male yellow croaker.

[0040] Embodiment 2:

[0041] The embodiment of the present invention provides an all-male large yellow croaker, which is produced by pairing a YY-type male large yellow croaker with an XX-type female large yellow croaker, so that all the offspring are normal XY-type male large yellow croaker.

[0042] A method for cultivating an all-male large yellow croaker supporting preservation system, based on the above all-male large yellow croaker, comprises the following steps:

[0043] Step 1: First, the male large yellow croaker is induced to transform with estrogen, wherein the estrogen is estradiol E2, to obtain an XY type female large yellow croaker;

[0044] Step 2: Then, the sex-transformed XY-type female large yellow croaker is subjected to gynogenetic development, and the offspring produced include YY-type male genetic large yellow croaker. Specifically, genetic sex PCR is used for identification, and then YY-type male genetic large yellow croaker is obtained through screening;

[0045] Step 3: Pair the YY male yellow croaker homozygous line with the normal XX female yellow croaker, and all the offspring produced will be normal XY male yellow croaker.

[0046] Embodiment three:

[0047] The embodiment of the present invention provides an all-male large yellow croaker, which is produced by pairing a YY-type male large yellow croaker with an XX-type female large yellow croaker, so that all the offspring are normal XY-type male large yellow croaker.

[0048] A method for cultivating an all-male large yellow croaker supporting preservation system, based on the above all-male large yellow croaker, comprises the following steps:

[0049] Step 1: First, the male large yellow croaker is induced to transform with estrogen, wherein the estrogen is estradiol E2, to obtain an XY type female large yellow croaker;

[0050] Step 2: Then, the sex-transformed XY-type female large yellow croaker is subjected to gynogenetic development, and the offspring produced include YY-type male genetic large yellow croaker. Specifically, genetic sex PCR is used for identification, and then YY-type male genetic large yellow croaker is obtained through screening;

[0051] Step three: Induce feminization of the YY male large yellow croaker homozygous line to obtain the YY female large yellow croaker homozygous line, and use the YY female large yellow croaker homozygous line to construct a YY male homozygous line on a large scale.

[0052] Embodiment 4: Figure 1 As shown:

[0053] (1) Functional female fish induction technology for large yellow croaker

[0054] The invention establishes a technology for inducing and cultivating genetic male fish (XY♂) and super male fish (YY♂) into functional female fish (XY♀, YY♀) by using estradiol E2, the genetic male fish feminization induction rate is more than 90%, and the survival rate of the treatment group is more than 50%.

[0055] (2) Breeding of all-male large yellow croaker

[0056] A functional female fish (XY♀) population was constructed by E2 induction. The functional female fish (XY♀) was paired with the original male fish (XY♂), and the genetic sex PCR was used to identify and screen the homozygous male population (YY♂) of large yellow croaker. In 2021, male homozygous (YY♂) was paired with the original female fish (XX♀), and 251,800 all-male seedlings were bred. The seedlings and one-year-old fish were tested, and the masculinization rate was 93.3%. In 2022, 46 male homozygous (YY♂) were paired with the original female fish (XX♀), and 899,000 all-male large yellow croakers were bred. The masculinization rate of seedlings was 100%, and the masculinization rate of one-year-old fish was 93.3% to 100%.

[0057] (3) Construction of supermale conservation lines

[0058] Using male homozygous lines (YY♂) and male homozygous conversion lines (YY♀) of large yellow croaker, large-scale construction of male homozygous lines (YY♂) was carried out. In 2021, 6 male homozygous individuals (YY♂) and 6 male homozygous conversion individuals (YY♀) were selected to test large-scale breeding of male homozygous lines, breeding 128,000 indoor seedlings; E2 feminization induction was carried out on 45-day-old male homozygous individuals at the same time to establish male homozygous conversion lines (YY♀). In 2023, 7 male homozygous individuals (YY♂) were selected and matched with 20 male homozygous conversion individuals (YY♀) to breed 65,800 male homozygous lines; 3,000 male homozygous gynogenetic lines were constructed using gynogenetic technology using 6 male homozygous conversion line individuals (YY♀) as parents.

[0059] In summary, the super male preservation line construction technology of the present invention not only improves the reproductive efficiency of large yellow croaker, but also ensures the gender consistency of offspring through gender control technology, providing a new technical means for the breeding industry of large yellow croaker. Through the breeding method of the present invention, the breeding of female large yellow croaker can be effectively avoided, thereby reducing breeding costs and improving economic benefits. In addition, the present invention also provides a possibility for the genetic improvement of large yellow croaker, and by controlling specific genetic traits, a variety that is more adapted to market demand can be cultivated. The embodiments of the present invention show how to achieve gender control and genetic homozygosity of large yellow croaker through scientific methods, providing new ideas and solutions for the sustainable development of aquaculture.

[0060] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An all-male large yellow croaker, characterized in that: By pairing a YY-type male yellow croaker with an XX-type female yellow croaker, all the offspring produced are normal XY-type male yellow croakers.

2. A method for cultivating a supporting preservation system of all-male large yellow croaker, based on the all-male large yellow croaker described in claim 1, characterized in that: The following steps are involved: Step 1: First, use estrogen to induce sexual transformation of male large yellow croaker to obtain XY type female large yellow croaker; Step 2: Then pair the sex-transformed XY female yellow croaker with the normal XY male yellow croaker, and the offspring produced include YY male genetic yellow croaker; Step 3: Pair the YY-type male yellow croaker with the normal XX-type female yellow croaker, and all the offspring produced will be normal XY-type male yellow croaker.

3. The method for cultivating a supporting preservation system of all-male large yellow croaker according to claim 2, characterized in that: In step 1, the estrogen is estradiol E2.

4. The method for cultivating a supporting preservation system of all-male large yellow croaker according to claim 2, characterized in that: In step 2, genetic sex PCR is used for identification, and then YY type male genetic large yellow croaker is obtained through screening.

5. A method for cultivating a supporting preservation system of all-male large yellow croaker, based on the all-male large yellow croaker according to claim 1, characterized in that: The following steps are involved: Step 1: First, use estrogen to induce sexual transformation of male large yellow croaker to obtain XY type female large yellow croaker; Step 2: Then, the sex-transformed XY female large yellow croaker is subjected to gynogenetic development, and the offspring produced include YY male genetic large yellow croaker; Step 3: Pair the YY male yellow croaker homozygous line with the normal XX female yellow croaker, and all the offspring produced will be normal XY male yellow croaker.

6. The method for cultivating a supporting preservation system of all-male large yellow croaker according to claim 5, characterized in that: In step 1, the estrogen is estradiol E2.

7. The method for cultivating a supporting preservation system of all-male large yellow croaker according to claim 5, characterized in that: In step 2, genetic sex PCR is used for identification, and then YY type male genetic large yellow croaker is obtained through screening.

8. A method for cultivating an all-male large yellow croaker supporting preservation system, based on the all-male large yellow croaker according to claim 1, characterized in that: The following steps are involved: Step 1: First, use estrogen to induce sexual transformation of male large yellow croaker to obtain XY type female large yellow croaker; Step 2: Then, the sex-transformed XY female large yellow croaker is subjected to gynogenetic development, and the offspring produced include YY male genetic large yellow croaker; Step three: Induce feminization of the YY male large yellow croaker homozygous line to obtain the YY female large yellow croaker homozygous line, and use the YY female large yellow croaker homozygous line to construct a YY male homozygous line on a large scale.

9. The method for cultivating a supporting preservation system of all-male large yellow croaker according to claim 8, characterized in that: In step 1, the estrogen is estradiol E2.

10. The method for cultivating a supporting preservation system of all-male large yellow croaker according to claim 8, characterized in that: In step 2, genetic sex PCR is used for identification, and then YY type male genetic large yellow croaker is obtained through screening.

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