Process for restoring damaged soil ecology by lincomycin waste liquid fertilizer

Abstract

The invention discloses a process for repairing damaged soil ecology by lincomycin waste liquid fertilizer, and relates to the technical field of soil remediation, which comprises the following specific steps: step 1, screening and culturing microbial decomposition flora, the invention has the following advantages: through the chelation of the polyaspartic acid derivative on metal ions and the promotion effect on nutrient absorption, the release and transmission of nutrients are precisely regulated and controlled, so that crops can absorb and utilize the nutrients more efficiently; the nano composite material and the specific biological stimulant containing various beneficial microbial strains and natural plant extracts are jointly fused into the liquid fertilizer, the microbial strains in the specific biological stimulant activate the soil biological environment, the natural plant extracts assist plant growth to resist diseases, and the nano composite material and the natural plant extracts have a synergistic effect, so that the liquid fertilizer has a good application prospect. Physicochemical properties and biological activity of soil are optimized, excellent soil micro-ecology is created for plant growth, healthy and strong growth of plants is promoted, and restoration of damaged soil is enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of soil restoration, in particular to a process for restoring damaged soil ecology by using lincomycin waste liquid liquid fertilizer. Background Art

[0002] With the rapid development of the pharmaceutical industry, the scale of lincomycin production by Streptomyces fermentation has continued to expand. However, the large amount of lincomycin waste liquid generated in this process has brought severe environmental challenges. Lincomycin waste liquid contains not only nutrients that are not fully utilized, but also residual lincomycin and other organic and inorganic impurities. If it is directly discharged without proper treatment, it will cause serious pollution to the ecological environment such as soil and water bodies, such as imbalance of soil microbial communities and eutrophication of water bodies. The existing technology has certain defects. First, the existing liquid fertilizer technology focuses more on nutrient supply, ignores the construction of soil water and fertilizer retention system, lacks a synergistic combination of ingredients, and is difficult to optimize soil microecology to promote plant growth and soil remediation. Secondly, the existing lincomycin waste liquid treatment technology does not fully degrade the waste liquid, and the purification effect is limited. It is impossible to provide high-quality basic liquid for liquid fertilizer preparation to ensure the repair efficiency of damaged soil. Moreover, the traditional damaged soil remediation method is single, and does not comprehensively consider the physical, chemical and biological needs of the soil. There is a lack of system integration solutions, and it is difficult to achieve comprehensive repair and reconstruction of the soil ecosystem. For this reason, we propose a process for repairing damaged soil ecology with lincomycin waste liquid liquid fertilizer. Summary of the invention

[0003] The purpose of the invention is to provide a process for repairing damaged soil ecology by using lincomycin waste liquid liquid fertilizer.

[0004] In order to solve the problems raised in the above background technology, the present invention provides the following technical solution: a process for repairing damaged soil ecology with lincomycin waste liquid liquid fertilizer, the process for repairing damaged soil ecology comprises the following specific steps: Step 1: Screening and culturing microbial decomposition flora, collecting soil samples at the drainage outlet of a lincomycin production workshop, and then preparing a selective culture medium with lincomycin as the sole carbon source and nitrogen source, and then inoculating the collected soil samples into the selective culture medium, monitoring the optical density value during the culture process, and when the OD value reaches a predetermined value (the microorganism is in the early logarithmic phase), transferring the bacterial solution to a new culture medium containing a higher concentration of lincomycin, and then inoculating it into culture media with different concentrations of lincomycin in sequence to adapt the microorganisms to the high concentration lincomycin environment, and then inoculating the cultured microbial decomposition flora into the lincomycin waste liquid, adjusting the pH value of the waste liquid, and controlling the temperature and stirring speed for reaction; Step 2: Filter the waste liquid after the reaction and decomposition in step 1 with a plate and frame filter to remove the bacteria and undecomposed solid impurities in the waste liquid, obtain clarified decomposed waste liquid, and then pass the filtered waste liquid through an adsorption column filled with activated carbon to further remove residual pigments, odor substances and a small amount of undecomposed organic matter; Step 3, preparing a sodium alginate solution, dissolving sodium alginate in deionized water to form a sodium alginate solution and stirring until completely dissolved, then preparing a montmorillonite suspension, placing montmorillonite powder in water at a certain mass ratio and ultrasonically treating it to make it uniformly dispersed, then synthesizing a polyaspartic acid derivative, thermally polycondensing aspartic acid and grafting copolymerizing it with an amino-containing small molecule compound, and finally synthesizing a nanocomposite material, slowly dripping the montmorillonite suspension into the sodium alginate solution and stirring, then adding the montmorillonite suspension, the polyaspartic acid derivative and dripping a calcium chloride solution for cross-linking, stirring to obtain a gel, and then washing, drying and grinding into powder; Step 4: Select specific microbial strains with plant growth promotion and soil remediation functions, culture them in culture medium to logarithmic growth phase, collect the bacteria, dry the bacteria by low-temperature freeze-drying technology to obtain microbial powder, mix the microbial powder with rosin acid derivatives extracted from Masson pine with antibacterial and growth-promoting effects in proportion, and then use ultrasonic dispersion technology to evenly disperse the mixture in a phosphate buffer solution to form a specific biostimulant; Step 5: using the decomposed waste liquid treated in step 2 as a base liquid, adding urea to supplement nitrogen, adding potassium dihydrogen phosphate to supplement phosphorus, adding potassium sulfate to supplement potassium, and then adding zinc sulfate and boric acid, mixing and stirring to obtain a preliminary liquid fertilizer solution, then adding the multifunctional nanocomposite material in proportion, stirring at room temperature until uniform, so that the nanocomposite material is uniformly dispersed, and finally adding the specific biostimulant in proportion and stirring to obtain a lincomycin waste liquid-based liquid fertilizer with high water and fertilizer retention performance and capable of effectively stimulating plant growth and soil remediation; Step six, repair the damaged soil. Before applying liquid fertilizer, first plow the damaged soil, then spray the prepared liquid fertilizer evenly on the soil surface, and mix it with a rotary tiller to a certain depth of soil layer. During the repair, regularly supplement with a mixed bacterial agent of Bacillus megaterium, Bacillus gelatinosa, round brown nitrogen-fixing bacteria and Trichoderma, and add decomposed compost as organic material. Then plant alfalfa, a suitable pioneer plant. Pay attention to the moisture content range of the soil during the growth process.

[0005] As a further solution of the present invention: in the step 1, the initial concentration of lincomycin in the selective culture medium is 50 mg / L-100 mg / L, the culture temperature is 28° C.-30° C., and the pH value is 7.0-7.5.

[0006] As a further scheme of the present invention: in the step 1, when the optical density value OD reaches 0.2-0.3, the culture medium is transferred to a new culture medium containing 150 mg / L-200 mg / L lincomycin at a volume ratio of 10%-15% of the bacterial liquid, and then transferred to culture media containing lincomycin concentrations of 250 mg / L-300 mg / L, 350 mg / L-400 mg / L, 450 mg / L-500 mg / L and 600 mg / L-700 mg / L in sequence, with an interval of 2-4 days between each transfer, the volume ratio of the microbial decomposition flora inoculated into the lincomycin waste liquid is 1:10-15, the initial pH of the waste liquid is adjusted to 7.0-7.5 during the reaction, the temperature is controlled to 30°C-32°C, the stirring speed is 100 r / min-120 r / min, and the reaction time is 72h-96h.

[0007] As a further solution of the present invention: in the step 2, the pore size of the filter cloth of the plate and frame filter is 5μm-10μm, the filling amount of the activated carbon adsorption column is 60%-70% of the volume of the adsorption column, and the flow rate is controlled to be 1BV / h-2BV / h.

[0008] As a further scheme of the present invention: in the step three, the mass fraction of the sodium alginate solution is 8%-10%, the dissolution temperature is 60°C-65°C, the mass ratio of montmorillonite powder to water when preparing the montmorillonite suspension is 1:20-22, the ultrasonic power is 300W-500W, the ultrasonic time is 45min-60min, and the aspartic acid thermal condensation temperature is 180°C-200°C and the time is 2h-3h when synthesizing the polyaspartic acid derivative, and the polyaspartic acid derivative is obtained, and the graft copolymerization temperature with ethylenediamine is 80°C-100°C and the time is 4h-6h.

[0009] As a further scheme of the present invention: in the step three, when synthesizing the nanocomposite material, the montmorillonite suspension is slowly dripped into the sodium alginate solution and stirred at 200r / min-300r / min for 30min-50min, the amount of montmorillonite suspension added is 30%-40% of the total mass of the sodium alginate solution, the amount of polyaspartic acid derivative added is 10%-15% of the total mass of the sodium alginate solution, the volume of the calcium chloride solution is 1 / 5-1 / 3 of the volume of the sodium alginate solution, the stirring speed during cross-linking is 100r / min-150r / min, the stirring time is 2h-3h, the gel is washed 3-5 times, the drying temperature is 60℃-70℃, and the drying time is 12h-24h.

[0010] As a further scheme of the present invention: in the step 4, the microbial strains are arbuscular mycorrhizal fungi, Bacillus and Pseudomonas, the mass ratio of microbial powder to rosin acid derivative is 1:3-5, the concentration of phosphate buffer solution is 0.05mol / L-0.1mol / L, the pH value is 7.0-7.5, the ultrasonic power is 200W-300W, and the ultrasonic time is 20min-30min.

[0011] As a further solution of the present invention: in the step 5, urea is added to make the nitrogen content 10%-15%, potassium dihydrogen phosphate is added to make the phosphorus content 5%-8%, potassium sulfate is added to make the potassium content 10%-12%, zinc sulfate is added in an amount of 0.1%-0.3% of the total volume of the waste liquid, boric acid is added in an amount of 0.05%-0.1% of the total volume of the waste liquid, the multifunctional nano-composite material is added in an amount of 8%-10% of the total mass of the base liquid, the stirring speed is 150r / min-200r / min, the stirring time is 45min-60min, the specific biostimulant is added in a volume ratio of 5%-8% of the total mass of the base liquid, and the stirring time is 30min-45min.

[0012] As a further solution of the present invention: in step 6, the soil tillage depth is 20cm-30cm, the liquid fertilizer application rate is 200L-500L per mu, the mixed soil layer is 5cm-20cm, and the microbial agent application rate is 1.0×10 5 -1.0×10 7 The amount of compost added is 200kg-300kg per mu, the alfalfa sowing amount is 1kg-2kg per mu, and the soil moisture content is maintained at 60%-80%.

[0013] By adopting the above technical solution, compared with the prior art, the beneficial effects of the present invention are: 1. The present invention uses the special structure and properties of natural polysaccharide sodium alginate in the multifunctional nanocomposite material to build a water-retaining network in the soil, effectively locks in water, and prevents excessive loss of water, thereby significantly improving the water-retaining capacity of the soil. Through the unique layered structure and large specific surface area of ​​montmorillonite, abundant adsorption sites are formed in the soil, which strongly adsorb nutrient ions in liquid fertilizers, reduce nutrient leaching, and greatly enhance the fertilizer-retaining performance of the soil. Through the chelation of metal ions by polyaspartic acid derivatives and the promotion of nutrient absorption, the release and transmission of nutrients are precisely regulated, so that crops can absorb and utilize nutrients more efficiently. By integrating the nanocomposite material and a specific biostimulant containing a variety of beneficial microbial strains and natural plant extracts into the liquid fertilizer, the microbial strains in the specific biostimulant activate the soil biological environment and promote nutrient conversion and circulation. The natural plant extract helps plant growth to resist diseases, and synergizes with the nanocomposite material to optimize the physical and chemical properties and biological activity of the soil, create an excellent soil microecology for plant growth, promote plant growth, and enhance the repair and improvement effect of damaged soil. 2. The present invention can effectively degrade lincomycin by treating lincomycin waste liquid with special microbial decomposition flora. The microbial flora can adapt to high-concentration lincomycin and decompose it into beneficial components such as small-molecule organic acids, inorganic ions and carbon dioxide after specific environmental screening and gradual concentration acclimation, thereby reducing the content of lincomycin in the waste liquid and reducing its potential harm to the ecological environment. At the same time, the plate-and-frame filter can remove the bacteria and undecomposed solid impurities in the waste liquid, thereby ensuring the purity of the waste liquid system. The activated carbon adsorption column relies on its own strong adsorption performance to remove residual pigments, odor substances and a small amount of undecomposed organic matter, thereby further optimizing the quality of the waste liquid. These materials and treatment methods cooperate with each other to provide a high-quality base liquid for the subsequent preparation of liquid fertilizers, thereby improving the repair efficiency of liquid fertilizers on damaged soils and promoting nutrient circulation and microbial activity recovery in the soil. 3. The present invention tills the damaged soil and sprays liquid fertilizer to fully mix the soil and fertilizer. The loose soil structure provides a favorable space for microbial activities and plant root growth. The added specific microbial agents multiply in the soil. Phosphate-solubilizing bacteria, potassium-solubilizing bacteria, and nitrogen-fixing bacteria obtain nutrients such as phosphorus, potassium, and nitrogen from soil minerals and air, respectively, and convert them into forms that can be absorbed by plants. Trichoderma effectively inhibits pathogens, enhances soil biological activity and health, and mature compost provides rich carbon sources and nutrients for microorganisms, further promoting their activation and proliferation, improving soil fertility and self-repairing ability. The pioneer plant alfalfa grows in this environment, and its root secretions nourish microorganisms. The root system interpenetrates to improve the physical structure of the soil, increase porosity and water-stable aggregates, and reduce soil erosion and compaction. Continuous monitoring and timely management are carried out throughout the process to ensure the healthy growth of plants. After one growing season or longer, the physical structure of the soil is optimized, the chemical properties are balanced, and the biodiversity is enriched, thereby achieving comprehensive repair and reconstruction of the damaged soil ecosystem. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram in an embodiment of the present invention. DETAILED DESCRIPTION

[0015] The specific embodiments of the present invention will be further described below in conjunction with the accompanying drawings. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0016] In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0017] Please see attached Figure 1 The present invention provides a process for repairing damaged soil ecology by using lincomycin waste liquid liquid fertilizer, and the process for repairing damaged soil ecology comprises the following specific steps: Step 1: Screening and culturing microbial decomposition flora, collecting soil samples at the drainage outlet of a lincomycin production workshop, and then preparing a selective culture medium with lincomycin as the sole carbon source and nitrogen source, and then inoculating the collected soil samples into the selective culture medium, monitoring the optical density value during the culture process, and when the OD value reaches a predetermined value (the microorganism is in the early logarithmic phase), transferring the bacterial solution to a new culture medium containing a higher concentration of lincomycin, and then inoculating it into culture media with different concentrations of lincomycin in sequence to adapt the microorganisms to the high concentration lincomycin environment, and then inoculating the cultured microbial decomposition flora into the lincomycin waste liquid, adjusting the pH value of the waste liquid, and controlling the temperature and stirring speed for reaction; Step 2: Filter the waste liquid after the reaction and decomposition in step 1 with a plate and frame filter to remove the bacteria and undecomposed solid impurities in the waste liquid, obtain clarified decomposed waste liquid, and then pass the filtered waste liquid through an adsorption column filled with activated carbon to further remove residual pigments, odor substances and a small amount of undecomposed organic matter; Step 3, preparing a sodium alginate solution, dissolving sodium alginate in deionized water to form a sodium alginate solution and stirring until completely dissolved, then preparing a montmorillonite suspension, placing montmorillonite powder in water at a certain mass ratio and ultrasonically treating it to make it uniformly dispersed, then synthesizing a polyaspartic acid derivative, thermally polycondensing aspartic acid and grafting copolymerizing it with an amino-containing small molecule compound, and finally synthesizing a nanocomposite material, slowly dripping the montmorillonite suspension into the sodium alginate solution and stirring, then adding the montmorillonite suspension, the polyaspartic acid derivative and dripping a calcium chloride solution for cross-linking, stirring to obtain a gel, and then washing, drying and grinding into powder; Step 4: Select specific microbial strains with plant growth promotion and soil remediation functions, culture them in culture medium to logarithmic growth phase, collect the bacteria, dry the bacteria by low-temperature freeze-drying technology to obtain microbial powder, mix the microbial powder with rosin acid derivatives extracted from Masson pine with antibacterial and growth-promoting effects in proportion, and then use ultrasonic dispersion technology to evenly disperse the mixture in a phosphate buffer solution to form a specific biostimulant; Step 5: using the decomposed waste liquid treated in step 2 as a base liquid, adding urea to supplement nitrogen, adding potassium dihydrogen phosphate to supplement phosphorus, adding potassium sulfate to supplement potassium, and then adding zinc sulfate and boric acid, mixing and stirring to obtain a preliminary liquid fertilizer solution, then adding the multifunctional nanocomposite material in proportion, stirring at room temperature until uniform, so that the nanocomposite material is uniformly dispersed, and finally adding the specific biostimulant in proportion and stirring to obtain a lincomycin waste liquid-based liquid fertilizer with high water and fertilizer retention performance and capable of effectively stimulating plant growth and soil remediation; Step six, repair the damaged soil. Before applying liquid fertilizer, first plow the damaged soil, then spray the prepared liquid fertilizer evenly on the soil surface, and mix it with a rotary tiller to a certain depth of soil layer. During the repair, regularly supplement with a mixed bacterial agent of Bacillus megaterium, Bacillus gelatinosa, round brown nitrogen-fixing bacteria and Trichoderma, and add decomposed compost as organic material. Then plant alfalfa, a suitable pioneer plant. Pay attention to the moisture content range of the soil during the growth process.

[0018] In one embodiment of the present invention: in step 1, the initial concentration of lincomycin in the selective culture medium is 50 mg / L-100 mg / L, the culture temperature is 28° C.-30° C., and the pH value is 7.0-7.5.

[0019] In one embodiment of the present invention: in step 1, when the optical density value OD reaches 0.2-0.3, the culture medium is transferred to a new culture medium containing 150 mg / L-200 mg / L lincomycin at a volume ratio of 10%-15% of the bacterial liquid, and then transferred to culture media containing lincomycin concentrations of 250 mg / L-300 mg / L, 350 mg / L-400 mg / L, 450 mg / L-500 mg / L and 600 mg / L-700 mg / L in sequence, with an interval of 2-4 days between each transfer, and the volume ratio of the microbial decomposition flora inoculated into the lincomycin waste liquid is 1:10-15. During the reaction of the waste liquid, the initial pH is adjusted to 7.0-7.5, the temperature is controlled to 30°C-32°C, the stirring speed is 100 r / min-120 r / min, and the reaction time is 72h-96h.

[0020] In one embodiment of the present invention: in step 2, the pore size of the filter cloth of the plate and frame filter is 5μm-10μm, the filling amount of the activated carbon adsorption column is 60%-70% of the volume of the adsorption column, and the flow rate is controlled to be 1BV / h-2BV / h.

[0021] In one embodiment of the present invention: in step three, the mass fraction of sodium alginate solution is 8%-10%, the dissolution temperature is 60°C-65°C, the mass ratio of montmorillonite powder to water in the preparation of montmorillonite suspension is 1:20-22, the ultrasonic power is 300W-500W, the ultrasonic time is 45min-60min, and the aspartic acid thermal condensation temperature is 180°C-200°C for 2h-3h during the synthesis of polyaspartic acid derivatives to obtain polyaspartic acid derivatives, and the graft copolymerization temperature with ethylenediamine is 80°C-100°C for 4h-6h.

[0022] In one embodiment of the present invention: in step three, when synthesizing the nanocomposite material, the montmorillonite suspension is slowly dripped into the sodium alginate solution and stirred at 200r / min-300r / min for 30min-50min, the amount of montmorillonite suspension added is 30%-40% of the total mass of the sodium alginate solution, the amount of polyaspartic acid derivative added is 10%-15% of the total mass of the sodium alginate solution, the volume of the calcium chloride solution is 1 / 5-1 / 3 of the volume of the sodium alginate solution, the stirring speed during cross-linking is 100r / min-150r / min, the stirring time is 2h-3h, the gel is washed 3-5 times, the drying temperature is 60℃-70℃, and the drying time is 12h-24h.

[0023] In one embodiment of the present invention: in step 4, the microbial strains are arbuscular mycorrhizal fungi, Bacillus and Pseudomonas, the mass ratio of microbial powder to rosin acid derivative is 1:3-5, the concentration of phosphate buffer solution is 0.05mol / L-0.1mol / L, the pH value is 7.0-7.5, the ultrasonic power is 200W-300W, and the ultrasonic time is 20min-30min.

[0024] In one embodiment of the present invention: in step five, urea is added to make the nitrogen content 10%-15%, potassium dihydrogen phosphate is added to make the phosphorus content 5%-8%, potassium sulfate is added to make the potassium content 10%-12%, zinc sulfate is added in an amount of 0.1%-0.3% of the total volume of the waste liquid, boric acid is added in an amount of 0.05%-0.1% of the total volume of the waste liquid, the multifunctional nano-composite material is added in an amount of 8%-10% of the total mass of the base liquid, the stirring speed is 150r / min-200r / min, the stirring time is 45min-60min, the specific biostimulant is added in a volume ratio of 5%-8% of the total mass of the base liquid, and the stirring time is 30min-45min.

[0025] In one embodiment of the present invention, in step 6, the soil is tilled to a depth of 20 cm-30 cm, the amount of liquid fertilizer applied is 200 L-500 L per mu, the mixed soil layer is 5 cm-20 cm, and the amount of microbial agent used is 1.0×10 5 -1.0×10 7 The amount of compost added is 200kg-300kg per mu, the alfalfa sowing amount is 1kg-2kg per mu, and the soil moisture content is maintained at 60%-80%.

[0026] In one embodiment of the present invention: in step one, in the process of transferring the microbial decomposition flora to lincomycin culture media of different concentrations, each time the transfer is performed, a gene sequencing analysis is performed on the microbial flora to screen out a microbial subpopulation that undergoes specific gene mutations in the process of adapting to high-concentration lincomycin and is associated with enhanced gene expression of key enzymes for lincomycin degradation. Subsequently, only this subpopulation is used for waste liquid treatment to improve the degradation efficiency and stability of lincomycin.

[0027] In one embodiment of the present invention: in step 2, the filter cloth of the plate and frame filter adopts a gradient pore size structure, wherein the pore size near the waste liquid inlet side is 10μm-15μm, and the pore size near the outlet side is 5μm-10μm, so that larger particulate impurities are first retained during the filtration process, and then gradually refined and filtered, thereby reducing the risk of filter cloth clogging and improving filtration efficiency.

[0028] In one embodiment of the present invention: In step three, when synthesizing the nanocomposite material, a biodegradable cross-linking enhancer is added, and the cross-linking enhancer is modified cellulose nanocrystals, and the addition amount thereof is 5%-8% of the total mass of the sodium alginate solution, which can further improve the mechanical strength of the nanocomposite material and the controllability of its degradation cycle in the soil, making the soil structure improvement effect more lasting and environmentally friendly.

[0029] In one embodiment of the present invention: in step 4, before mixing the microbial powder with the rosin acid derivative, the microbial powder is subjected to plasma surface treatment, the treatment time is 10min-15min, the power is 100W-150W, so that the surface activity of the powder is enhanced, the binding force and dispersion uniformity with the rosin acid derivative and the subsequent buffer solution are improved, and the activity and stability of the specific biostimulant are enhanced.

[0030] In one embodiment of the present invention: the rosin acid derivative in step 4 is first subjected to nano-encapsulation treatment before being mixed with the microbial powder. The microcapsule wall material is a biodegradable polylactic acid-glycolic acid copolymer, and the microcapsule particle size is controlled at 100nm-200nm. This treatment can achieve the sustained release of the rosin acid derivative in the soil, prolong its action time and reduce the instantaneous impact on the activity of the microbial powder, continuously and stably exert antibacterial and growth-promoting effects, and improve the durability and stability of the soil remediation effect.

[0031] Example 1, please refer to the attached Figure 1First, soil samples were collected from the drainage outlet of the lincomycin production workshop, and a selective culture medium with lincomycin as the sole carbon and nitrogen source and an initial concentration of 100 mg / L was prepared. The soil samples were inoculated into the culture medium and placed in a constant temperature incubator at a temperature of 30°C and a pH of 7.5 for culture. When the OD value reached 0.3, the culture medium was transferred to a new culture medium containing 200 mg / L lincomycin at a volume ratio of 15%. Subsequently, the culture medium was inoculated into 300 mg / L, 400 mg / L, 500 mg / L and 700 mg / L culture media at intervals of 4 days each time to adapt the microorganisms to the 700 mg / L lincomycin environment. Then, the cultured microbial decomposition flora was inoculated into the lincomycin waste liquid at a volume ratio of 1:15, and the initial pH of the waste liquid was adjusted. H to 7.5, control the temperature to 32°C, react at a stirring speed of 120r / min for 96h, use a plate and frame filter to filter the waste liquid after the reaction decomposition, the pore size of the filter cloth selected is 10μm, obtain the clarified waste liquid after decomposition, let it pass through an activated carbon adsorption column with a filling volume of 70% of the adsorption column volume, and the flow rate is controlled to 2BV / h. Prepare a sodium alginate solution, dissolve sodium alginate in deionized water to form a solution with a mass fraction of 10%, stir at 65°C until completely dissolved, prepare a montmorillonite suspension, place montmorillonite powder in water at a mass ratio of 1:22, treat it with an ultrasonic power of 500W for 60min to make it evenly dispersed, synthesize a polyaspartic acid derivative, and heat-condense aspartic acid at 200°C for 3h to obtain a polyaspartic acid derivative , and then graft copolymerized with ethylenediamine containing amino small molecule compound under the catalysis of dibutyltin dilaurate at 100℃ for 6h, slowly dripped the montmorillonite suspension into the sodium alginate solution and stirred at 300r / min for 50min, the addition amount of montmorillonite suspension was 40% of the total mass of the sodium alginate solution, and then added polyaspartic acid derivatives and mixed evenly, the addition amount of polyaspartic acid derivatives was 15% of the total mass of the sodium alginate solution, and then added 1 / 3 of the volume of the sodium alginate solution with a mass fraction of 5% calcium chloride solution for cross-linking, stirred at 150r / min for 3h to obtain a gel, washed 5 times, dried at 70℃ for 24h and ground into powder, selected arbuscular mycorrhizal fungi, Bacillus and Pseudomonas, and cultured in culture medium respectively until the reaction was During the growth period, the cells were collected and dried by low-temperature freeze-drying technology to obtain microbial powder. The microbial powder was mixed with a rosin acid derivative with antibacterial and growth-promoting effects extracted from Pinus massoniana at a mass ratio of 1:5. The mixture was evenly dispersed in a phosphate buffer solution by ultrasonic dispersion technology. The concentration of the phosphate buffer solution was 0.1 mol / L, the pH value was 7.5, the ultrasonic power was 300 W, and the ultrasonic time was 30 min to form a specific biostimulant. The treated decomposed waste liquid was used as the base liquid, urea was added to make the nitrogen content reach 15%, potassium dihydrogen phosphate was added to make the phosphorus content reach 8%, and potassium sulfate was added to make the potassium content reach 12%. Then, zinc sulfate with a total volume of 0.3% of the total volume of the waste liquid and a total volume of 0.1% boric acid, mixed and stirred evenly to prepare a preliminary liquid fertilizer solution, then added 10% of the total mass of the base liquid multifunctional nanocomposite material, stirred at 200r / min at room temperature for 60min to make the nanocomposite material evenly dispersed, and finally added the specific biostimulant according to the volume ratio of 8% of the total mass of the base liquid, and continued to stir for 45min to obtain lincomycin waste liquid-based liquid fertilizer, which was used to repair the damaged soil. Before applying the liquid fertilizer, the damaged soil was first plowed to a depth of 30cm, and then the prepared liquid fertilizer was evenly sprayed on the soil surface at 500L per mu, and mixed with a rotary tiller to a 20cm soil layer, and a mixed bacterial agent of Bacillus megaterium, Bacillus gelatinus, round brown nitrogen-fixing bacteria and Trichoderma was regularly supplemented, with an amount of 1.0×10. 7 Live bacteria are added, and decomposed compost is added as organic material. The amount of compost added is 300kg per mu. Then alfalfa, a suitable pioneer plant, is planted at a sowing rate of 2kg per mu. The soil moisture content is maintained at 80% during the growth process.

[0032] Example 2, please refer to the attached Figure 1First, soil samples were collected from the drainage outlet of the lincomycin production workshop, and a selective culture medium with lincomycin as the sole carbon and nitrogen source and an initial concentration of 50 mg / L was prepared. The soil samples were inoculated into the culture medium and placed in a constant temperature incubator at a temperature of 28°C and a pH value of 7.0 for culture. When the OD value reached 0.2, the culture medium was transferred to a new culture medium containing 150 mg / L lincomycin at a volume ratio of 10%. Subsequently, the culture medium was inoculated into 250 mg / L, 350 mg / L, 450 mg / L and 600 mg / L culture media at intervals of 2 days each time to adapt the microorganisms to the 600 mg / L lincomycin environment. Then, the cultured microbial decomposition flora was inoculated into the lincomycin waste liquid at a volume ratio of 1:10, and the initial pH of the waste liquid was adjusted. H to 7.0, control the temperature to 30°C, react at a stirring speed of 100r / min for 72h, use a plate and frame filter to filter the waste liquid after the reaction decomposition, the pore size of the filter cloth selected is 5μm, obtain the clarified decomposition waste liquid, let it pass through an activated carbon adsorption column with a filling volume of 60% of the adsorption column volume, and control the flow rate to 1BV / h. Prepare a sodium alginate solution, dissolve sodium alginate in deionized water to form a solution with a mass fraction of 8%, stir at 60°C until completely dissolved, prepare a montmorillonite suspension, place montmorillonite powder in water at a mass ratio of 1:20, and treat it with an ultrasonic power of 300W for 45min to make it uniformly dispersed, synthesize a polyaspartic acid derivative, and heat-condense aspartic acid at 180°C for 2h to obtain a polyaspartic acid derivative. Then, it was grafted copolymerized with ethylenediamine containing amino small molecule compounds under the catalysis of dibutyltin dilaurate at 80°C for 4 hours, and the montmorillonite suspension was slowly dripped into the sodium alginate solution and stirred at 200r / min for 30 minutes. The addition amount of montmorillonite suspension was 30% of the total mass of the sodium alginate solution. Then, polyaspartic acid derivatives were added and mixed evenly. The addition amount of polyaspartic acid derivatives was 10% of the total mass of the sodium alginate solution. Subsequently, 5% calcium chloride solution with a mass fraction of 1 / 5 of the volume of the sodium alginate solution was added dropwise for cross-linking. The gel was stirred at 100r / min for 2 hours, washed 3 times, dried at 60°C for 12 hours and ground into powder. Arbuscular mycorrhizal fungi, Bacillus and Pseudomonas were selected and cultured in culture medium to logarithmic growth, respectively. Long-term, collect the bacteria, use low-temperature freeze-drying technology to dry the bacteria, obtain microbial powder, mix the microbial powder with abietic acid derivatives extracted from Pinus massoniana with antibacterial and growth-promoting effects in a mass ratio of 1:3, and use ultrasonic dispersion technology to evenly disperse the mixture in a phosphate buffer solution. The phosphate buffer solution concentration is 0.05 mol / L, the pH value is 7.0, the ultrasonic power is 200 W, and the ultrasonic time is 20 min to form a specific biostimulant. The treated decomposed waste liquid is used as the base liquid, urea is added to make the nitrogen content reach 10%, potassium dihydrogen phosphate is added to make the phosphorus content reach 5%, and potassium sulfate is added to make the potassium content reach 10%, and then 0.1% of the total volume of the waste liquid zinc sulfate and a total volume of 0.05% boric acid, mixed and stirred evenly to obtain a preliminary liquid fertilizer solution, then added 8% of the total mass of the base liquid multifunctional nanocomposite material, stirred at 150r / min at room temperature for 45min to make the nanocomposite material evenly dispersed, and finally added the specific biostimulant according to the volume ratio of 5% of the total mass of the base liquid, and continued to stir for 30min to obtain lincomycin waste liquid-based liquid fertilizer, which was used to repair the damaged soil. Before applying the liquid fertilizer, the damaged soil was first plowed to a depth of 20cm, and then the prepared liquid fertilizer was evenly sprayed on the soil surface at 200L per mu, and mixed with a rotary tiller to a 5cm soil layer, and a mixed bacterial agent of Bacillus megaterium, Bacillus gelatinus, round brown nitrogen-fixing bacteria and Trichoderma was regularly supplemented, with an amount of 1.0×10. 5 Live bacteria are added, and decomposed compost is added as organic material. The amount of compost added is 200kg per mu. Then alfalfa, a suitable pioneer plant, is planted at a sowing rate of 1 kg per mu. The soil moisture content is maintained at 60% during the growth process.

[0033] According to the above two groups of embodiments, it can be concluded that through the screening and cultivation of microbial decomposition flora, multi-step purification treatment of waste liquid, careful synthesis of multifunctional nanocomposite materials, scientific preparation of specific biostimulants, and reasonable liquid fertilizer compounding and damaged soil repair operations, the harmful substances in lincomycin waste liquid are effectively degraded, the water and fertilizer retention and soil repair performance of the liquid fertilizer are improved, and with the help of the coordinated cooperation of multiple technologies in each link, the good effect of using lincomycin waste liquid to prepare liquid fertilizer to repair the damaged soil ecology can be achieved. This process has significant technical advantages and broad application prospects.

[0034] Although the present invention is disclosed as above in terms of preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art may make possible changes and modifications without departing from the spirit and scope of the present invention. Therefore, any modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention shall fall within the scope of protection defined by the claims of the present invention.

Claims

1. A process for repairing damaged soil ecology using lincomycin waste liquid liquid fertilizer, characterized in that: The process for repairing damaged soil ecology includes the following specific steps: Step 1: Screening and culturing microbial decomposition flora, collecting soil samples at the drainage outlet of a lincomycin production workshop, and then preparing a selective culture medium with lincomycin as the sole carbon source and nitrogen source, and then inoculating the collected soil samples into the selective culture medium, monitoring the optical density value during the culture process, and when the OD value reaches a predetermined value (the microorganism is in the early logarithmic phase), transferring the bacterial solution to a new culture medium containing a higher concentration of lincomycin, and then inoculating it into culture media with different concentrations of lincomycin in sequence to adapt the microorganisms to the high concentration lincomycin environment, and then inoculating the cultured microbial decomposition flora into the lincomycin waste liquid, adjusting the pH value of the waste liquid, and controlling the temperature and stirring speed for reaction; Step 2: filtering the waste liquid after the reaction decomposition in step 1 with a plate and frame filter to obtain clarified decomposition waste liquid, and then passing the filtered waste liquid through an adsorption column filled with activated carbon; Step 3, preparing a sodium alginate solution, dissolving sodium alginate in deionized water to form a sodium alginate solution and stirring until completely dissolved, then preparing a montmorillonite suspension, placing montmorillonite powder in water at a certain mass ratio and ultrasonically treating it to make it uniformly dispersed, then synthesizing a polyaspartic acid derivative, thermally polycondensing aspartic acid and grafting copolymerizing it with an amino-containing small molecule compound, and finally synthesizing a nanocomposite material, slowly dripping the montmorillonite suspension into the sodium alginate solution and stirring, then adding the montmorillonite suspension, the polyaspartic acid derivative and dripping a calcium chloride solution for cross-linking, stirring to obtain a gel, and then washing, drying and grinding into powder; Step 4: Select specific microbial strains with plant growth promotion and soil remediation functions, culture them in culture medium to logarithmic growth phase, collect the bacteria, dry the bacteria by low-temperature freeze-drying technology to obtain microbial powder, mix the microbial powder with rosin acid derivatives extracted from Masson pine with antibacterial and growth-promoting effects in proportion, and then use ultrasonic dispersion technology to evenly disperse the mixture in a phosphate buffer solution to form a specific biostimulant; Step 5: using the decomposed waste liquid treated in step 2 as a base liquid, adding urea to supplement nitrogen, adding potassium dihydrogen phosphate to supplement phosphorus, adding potassium sulfate to supplement potassium, and then adding zinc sulfate and boric acid, mixing and stirring to obtain a preliminary liquid fertilizer solution, then adding the multifunctional nanocomposite material in proportion, stirring at room temperature until uniform, so that the nanocomposite material is uniformly dispersed, and finally adding the specific biostimulant in proportion and stirring to obtain a lincomycin waste liquid-based liquid fertilizer with high water and fertilizer retention performance and capable of effectively stimulating plant growth and soil remediation; Step six, repair the damaged soil. Before applying liquid fertilizer, first plow the damaged soil, then spray the prepared liquid fertilizer evenly on the soil surface, and mix it with a rotary tiller to a certain depth of soil layer. During the repair, regularly supplement with a mixed bacterial agent of Bacillus megaterium, Bacillus gelatinosa, round brown nitrogen-fixing bacteria and Trichoderma, and add decomposed compost as organic material. Then plant alfalfa, a suitable pioneer plant. Pay attention to the moisture content range of the soil during the growth process.

2. The process for repairing damaged soil ecology using lincomycin waste liquid liquid fertilizer according to claim 1, characterized in that: In the step 1, the initial concentration of lincomycin in the selective culture medium is 50 mg / L-100 mg / L, the culture temperature is 28° C.-30° C., and the pH value is 7.0-7.

5.

3. The process for repairing damaged soil ecology by using lincomycin waste liquid liquid fertilizer according to claim 1, characterized in that: In the step 1, when the optical density value OD reaches 0.2-0.3, the culture is transferred to a new culture medium containing 150 mg / L-200 mg / L lincomycin at a volume ratio of 10%-15% of the bacterial liquid, and then transferred to culture media containing lincomycin concentrations of 250 mg / L-300 mg / L, 350 mg / L-400 mg / L, 450 mg / L-500 mg / L and 600 mg / L-700 mg / L in sequence, with an interval of 2-4 days between each transfer, and the volume ratio of the microbial decomposition flora inoculated into the lincomycin waste liquid is 1:10-15. During the reaction of the waste liquid, the initial pH is adjusted to 7.0-7.5, the temperature is controlled to 30°C-32°C, the stirring speed is 100 r / min-120 r / min, and the reaction time is 72h-96h.

4. The process for repairing damaged soil ecology using lincomycin waste liquid liquid fertilizer according to claim 1, characterized in that: In the step 2, the pore size of the filter cloth of the plate and frame filter is 5 μm-10 μm, the filling amount of the activated carbon adsorption column is 60%-70% of the volume of the adsorption column, and the flow rate is controlled to be 1 BV / h-2 BV / h.

5. The process for repairing damaged soil ecology by using lincomycin waste liquid liquid fertilizer according to claim 1, characterized in that: In the step 3, the mass fraction of the sodium alginate solution is 8%-10%, the dissolution temperature is 60°C-65°C, the mass ratio of montmorillonite powder to water is 1:20-22 when preparing the montmorillonite suspension, the ultrasonic power is 300W-500W, the ultrasonic time is 45min-60min, and the aspartic acid thermal condensation temperature is 180°C-200°C and the time is 2h-3h when synthesizing the polyaspartic acid derivative. The polyaspartic acid derivative is obtained, and the graft copolymerization temperature with ethylenediamine is 80°C-100°C and the time is 4h-6h.

6. The process for repairing damaged soil ecology by using lincomycin waste liquid liquid fertilizer according to claim 1, characterized in that: In the step 3, when synthesizing the nanocomposite material, the montmorillonite suspension is slowly dripped into the sodium alginate solution and stirred at 200 r / min-300 r / min for 30 min-50 min, the amount of montmorillonite suspension added is 30%-40% of the total mass of the sodium alginate solution, the amount of polyaspartic acid derivative added is 10%-15% of the total mass of the sodium alginate solution, the volume of the calcium chloride solution is 1 / 5-1 / 3 of the volume of the sodium alginate solution, the stirring speed during cross-linking is 100 r / min-150 r / min, the stirring time is 2 h-3 h, the gel is washed 3-5 times, the drying temperature is 60° C.-70° C., and the drying time is 12 h-24 h.

7. The process for repairing damaged soil ecology by using lincomycin waste liquid liquid fertilizer according to claim 1, characterized in that: In the step 4, the microbial strains are arbuscular mycorrhizal fungi, Bacillus and Pseudomonas, the mass ratio of microbial powder to rosin acid derivative is 1:3-5, the concentration of phosphate buffer solution is 0.05mol / L-0.1mol / L, the pH value is 7.0-7.5, the ultrasonic power is 200W-300W, and the ultrasonic time is 20min-30min.

8. The process for repairing damaged soil ecology by using lincomycin waste liquid liquid fertilizer according to claim 1, characterized in that: In the step 5, urea is added to make the nitrogen content 10%-15%, potassium dihydrogen phosphate is added to make the phosphorus content 5%-8%, potassium sulfate is added to make the potassium content 10%-12%, zinc sulfate is added in an amount of 0.1%-0.3% of the total volume of the waste liquid, boric acid is added in an amount of 0.05%-0.1% of the total volume of the waste liquid, the multifunctional nano-composite material is added in an amount of 8%-10% of the total mass of the base liquid, the stirring speed is 150r / min-200r / min, the stirring time is 45min-60min, the specific biostimulant is added in a volume ratio of 5%-8% of the total mass of the base liquid, and the stirring time is 30min-45min.

9. The process for repairing damaged soil ecology by using lincomycin waste liquid liquid fertilizer according to claim 1, characterized in that: In step 6, the soil is tilled to a depth of 20 cm-30 cm, the amount of liquid fertilizer applied is 200 L-500 L per mu, the mixed soil layer is 5 cm-20 cm, and the amount of microbial agent used is 1.0 × 10 5 -1.0×10 7 The amount of compost added is 200kg-300kg per mu, the alfalfa sowing amount is 1kg-2kg per mu, and the soil moisture content is maintained at 60%-80%.

Images