Method for simulating biological stress reaction to induce gingkoes to quickly accumulate bilobalide

A stress response and ginkgolide technology, applied in the fields of biotechnology and botany, can solve the problem of accumulating ginkgolides that have not been reported in the literature

Inactive Publication Date: 2018-05-11
CHINA PHARM UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there is no literature report on the method of artificial cultivation combined with MeJA to induce rapid accumulation of ginkgolides in ginkgo

Method used

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  • Method for simulating biological stress reaction to induce gingkoes to quickly accumulate bilobalide
  • Method for simulating biological stress reaction to induce gingkoes to quickly accumulate bilobalide
  • Method for simulating biological stress reaction to induce gingkoes to quickly accumulate bilobalide

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Embodiment

[0030] In the present invention, the annual ginkgo seedlings of the same batch are planted in the nutrient soil of the same specification, cultivated under the same conditions, and adopt the method of combining 50% spraying and 50% watering before harvesting, and give Ginkgo 100, 200, 400mL of 400, 600, and 800μmol / L MeJA treatment solution was used to induce and stimulate Ginkgo biloba plants, and samples were collected at 5 time points of 12, 24, 48, 72, and 96 hours after treatment, freeze-dried, stored at -20°C, and liquid nitrogen Grind, extract with 70% methanol, and quantitatively detect the contents of ginkgolide A (GA) and ginkgolide B (GB) by HPLC-ELSD. The results showed that when the MeJA concentration was 100, 200, 400, 600, and 800 μmol / L, GA was significantly changed at different times, and the content of GB (induction concentration and content changes are shown in Figure 6 and 7 ). The results showed that after 72 hours of induced culture with MeJA solution ...

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Abstract

The invention provides a method for simulating a biological stress reaction to induce gingkoes to quickly accumulate bilobalide, and belongs to the technical field of biological techniques and botanytechniques. Gingko plants are cultured through simulation of natural growth conditions, jasmonic acid methyl ester (MeJA), namely a plant hormone, is adopted for inducing, and a high-performance liquid chromatography-evaporation light scattering method is adopted for detecting and analyzing the dynamic change condition of accumulation of the bilobalide. The change conditions of the bilobalide content are detected under different inducing concentration and time conditions, and therefore the concentration and inducing time of MeJA are determined, so that the optimum inducing condition of the MeJA for inducing fast accumulation of the bilobalide is screened out, really effective bases are provided for increasing the yield of the bilobalide, and a scientific basis is provided for treatment before harvesting of ginkgo leaves.

Description

technical field [0001] The invention relates to a method for inducing rapid accumulation of ginkgolides in ginkgo biloba by simulating biological stress response, and belongs to the technical fields of biotechnology and botany. Background technique [0002] Ginkgo biloba (Ginkgo biloba L) is a perennial gymnosperm plant of the genus Ginkgo in the Ginkgo family. It is one of the oldest extant tree species known as a "living fossil". Since modern times, the use value of ginkgo biloba extract and ginkgolide has been gradually developed and utilized. Now as a herbal medicine, ginkgo biloba extract has been widely sold at home and abroad. It can improve memory, increase blood circulation, and treat Alzheimer's disease, etc. effect. [0003] Ginkgolides (ginkgolides) are unique terpene active ingredients in Ginkgo biloba, including diterpene lactones ginkgolides A, B, C, J, K, L, M and sesquiterpene lactone bilobalide ( bilobalide). Ginkgolide can scavenge free radicals and inh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01N37/42A01P21/00
CPCA01G7/06A01N37/42
Inventor 陆续李萍林廷文郑晓燕
Owner CHINA PHARM UNIV
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