cDNA chip for determining gene expression information of crucifer under threatening conduction

Abstract

The invention discloses a cDNA chip for detecting gene expressing information of crucifer plants under biological or non-biological stress. The gene chip takes the cDNA fragment of Chinese cabbage gene under versity and special growing stage as the probe which is composed by probe with sequences shown in SEQ ID No.:1-221DNA and probe sequence from 3885 strips gene bank. The invention can be used not only in batch-detecting of gene expressing information of crucifer plants under biological or non-biological stress, screening of differential gene, but also can be used to build gene expressing profiles of cruciferous plants in different growing stages, different tissues and organs, and even idioblast. High-throughput detection of gene expression information can be carried out by the application of the gene chip, which is convenient and safe, with reliable and effective test results, and can save a lot of time, human, material and financial resources and has broad application prospects.

Description

technical field

[0001] The invention relates to a cDNA chip for detecting gene expression information of plants under stress conditions, in particular to a cDNA chip for detecting gene expression information of cruciferous plants under biological stresses such as diseases and insects and abiotic stresses such as drought and cold. Background technique

[0002] Using known gene information to specifically detect the expression information of the target gene in the sample to be tested is a commonly used detection method in the field of molecular biology, such as the commonly used Southern hybridization, Northern hybridization and PCR techniques. However, with the development of molecular biology, these techniques cannot simultaneously analyze thousands of genes under the same conditions, so they have certain limitations. It has become a trend to immobilize thousands of genes on the carrier by in situ synthesis (in situ synthesis) probes or synthesis of target gene fragments and...

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