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Adversity regulation gene HsCBL8 for improving anti-adversity characteristics of crops and cloning method thereof

A technology for regulating genes and cloning methods, which is applied in the fields of biological breeding and molecular biology, and can solve problems that affect crop yields and cannot grow food crops

Inactive Publication Date: 2014-12-24
ZHEJIANG SCI-TECH UNIV
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Problems solved by technology

[0005] The existing genetic engineering practice has proved that the use of downstream genes is difficult to solve the problem of molecular breeding for stress resistance (Bhatnagar-Mathur et al. 2008). Under waterlogging, salinity, etc.), the situation of production reduction and crop failure occurs from time to time, which greatly affects the yield of crops, and even some areas cannot grow food crops

Method used

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  • Adversity regulation gene HsCBL8 for improving anti-adversity characteristics of crops and cloning method thereof
  • Adversity regulation gene HsCBL8 for improving anti-adversity characteristics of crops and cloning method thereof
  • Adversity regulation gene HsCBL8 for improving anti-adversity characteristics of crops and cloning method thereof

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Embodiment Construction

[0041] The stress-regulated gene HsCBL8 of the present invention will be described in detail below in conjunction with the specific cloning process.

[0042] The first is to obtain cDNA: the annual wild barley seeds on the Qinghai-Tibet Plateau are cultured to the 3-leaf stage through fine sand, transplanted into liquid culture vessels, and treated with high salt (200mM), low pH (4.0) and low temperature (0-4°C) At 72 hours, the whole plant of the seedlings was taken and extracted with TaKaRa RNAiso Plus to extract total RNA, and then the first strand of cDNA was synthesized using reverse transcriptase (Takara, Dalian, D2680S).

[0043] The synthesis of cDNA is generally grasped by those skilled in the art. Specifically in the present invention, the synthesis reaction system of the first strand is as follows:

[0044] Total RNA 1μl

[0045] 5×M-MLV Buffer 2μl

[0046] Oligod(T) / linker (10μM) 1μl

[0047] dNTP Mixture (10mM each) 1μl

[0048] RNase Inhibitor 0.25μl

[0049] ...

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Abstract

The invention discloses an adversity regulation gene HsCBL8 for improving anti-adversity characteristics of crops. The adversity regulation gene HsCBL8 has a sequence shown in a sequence table SEQ ID No. 1. Correspondingly, the invention discloses a cloning method of the gene. The adversity regulation gene HsCBL8 disclosed by the invention is an upstream gene of a biological and non-biological stress response route of the various crops, can be used for regulating the stress reaction of various adversities, and is significantly beneficial for the anti-adversity breeding research of the crops.

Description

technical field [0001] The invention relates to a regulating gene capable of improving stress resistance characteristics of crops, and belongs to the fields of biological breeding and molecular biology. Background technique [0002] The complex and diverse natural environment has always been an important external factor affecting the yield and quality of crops. Drought, freezing damage, salinity, and acidic soil are the main environmental stress factors that plants suffer in nature, which seriously affect the growth and yield of crops. The frequent disastrous weather in recent years has seriously affected the yield of crops. [0003] In response to the above problems, there are two main solutions. One is to make great efforts to improve the natural growth conditions, such as improving soil quality and increasing forest density to prevent extreme weather. Long-term investment can only be realized, and it only has macroscopic significance, and has little effect on specific cr...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/10
Inventor 郭万里梁宗锁杨东风陈绍宁金伟波吴芳丽徐涛吕洪飞祁哲晨
Owner ZHEJIANG SCI-TECH UNIV
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