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Platform for developing soil-borne plant pathogen inhibiting microbial consortia

A technology for plant pathogens and microbial flora, applied in microorganism-based methods, microbial determination/inspection, microorganisms, etc., can solve problems such as low protection ability and single-strain inoculants cannot provide disease inhibition levels.

Pending Publication Date: 2021-07-30
RGT UNIV OF MINNESOTA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, single-strain inoculants may not provide sufficient levels of disease suppression to meet market demands
The ability of a single microbial strain to provide protection against any possible so

Method used

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  • Platform for developing soil-borne plant pathogen inhibiting microbial consortia
  • Platform for developing soil-borne plant pathogen inhibiting microbial consortia
  • Platform for developing soil-borne plant pathogen inhibiting microbial consortia

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0387] Example 1: Soil-borne Plant Pathogen Inhibitory Microflora (SPPIMC) Development Platform

[0388] The SPPIMC platform enables the identification of soil microorganisms with phytopathogen inhibitory activity. In addition, the platform generates multi-strain compositions (interchangeably referred to herein as "flora") comprising two or more phytopathogen-inhibiting microorganisms that are comparable to individual microorganisms Possesses superior properties (eg, the ability to inhibit a wider range of plant pathogens and to utilize a wider range of nutrients). The microbial flora developed using this platform and its excellent properties are described in Example 9.

[0389] Figure 14 A flow chart of the Soil-borne Plant Pathogen Suppressed Microbiota (SPPIMC) development platform is shown. The first step of the platform involves the creation of enriched microbial libraries. Next, the pathogen inhibitory activity of each isolate was determined. Subsequently, a multid...

example 2

[0390] Example 2: Creation of a bacterial library exhibiting antimicrobial activity against plant pathogens

[0391] Over a thousand isolates of Streptomyces, Bacillus, Fusarium and Pseudomonas were collected from agricultural soils, forest soils, North American temperate grassland soils, wetland soils, savannah soils and peat soils across all continents except Asia.

[0392] Bacteria are isolated using standard nutrient media (including water agar, oat agar, sodium caseinate agar, etc.). Habitat selection should both capture a broad range of ecological conditions and target the following environments: i) suppressor phenotypes may be enriched (Kinkel, L.L.), Schlatter, D.S. (Schlatter, D.S. ), Baker, M.B. (Bakker, M.B.) and Arenz, B. (Arenz, B.) (2012), Streptomyces competition and coevolution in relation to disease suppression, Research in Microbiology: dx.doi.org / 10.1016 / j.resmic.2012.07.005); or ii) long-term agricultural management was implemented.

[0393] Microbial iso...

example 3

[0394] Example 3: Characterization of isolates of a bacterial library of plant pathogen antimicrobial activity ("Determination of Pathogen Inhibitory Activity").

[0395] like Figure 14 Each microorganism in the library was subjected to a node involving "Multidimensional Ecological Function Balance (MEFB) node analysis", ie "Determining Pathogen Inhibitory Activity" as described below, as shown in .

[0396] method:

[0397] Fungal pathogen working stock isolates were grown on a benchtop (22°C-25°C) on 0.5x potato dextrose agar (15ml) for two days (Rhizoctonia and Sclerotinia) or five days (Grain Fusarium and Fusarium oxysporum). Fusarium solani cultures were grown on SMDA (Soy Milk Dextrose Agar) in the dark at (22°C-25°C) for approximately 20 days. Pythium was grown on a benchtop (22°C-25°C) on V8 agar for two days. Phytophthora were grown on V8 agar for two days on a benchtop (22°C-25°C). Pathogens tested included those of scab and Verticillium wilt (V. dahliae or Ver...

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Abstract

The disclosure relates to a systemic platform for developing soil-borne plant pathogen inhibiting microbial consortia. The platform utilizes multivariate computer modelling and multidimensional ecological function balancing (MEFB) nodal analysis to develop microbial consortia, consortia, and inoculants. The disclosure further relates to a prescriptive biocontrol system that will enable farmers to have site-specific agricultural biologics developed for their specific site and crop of interest.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS [0002] This application claims US Provisional Application No. 62 / 858,446, filed June 7, 2019; US Provisional Application No. 62 / 713,394, filed August 1, 2018; and US Provisional Application No. 62 / 713,394, filed July 25, 2018 62 / 703,060, the contents of each of which are incorporated herein by reference in their entirety for all purposes. [0003] Government funding [0004] This invention was made with government support under MCB-9977907 awarded by the US National Science Foundation and 2006-35319-17445 awarded by the USDA National Institute of Food and Agriculture. The government has certain rights in this invention. technical field [0005] The present disclosure relates to a system platform for developing soil-borne plant pathogen-inhibiting microbial flora. The platform utilizes multivariate computer modeling and multidimensional ecological function balance (MEFB) node analysis to develop microbial flora and inoculan...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01N63/28A01N63/22C12N15/10
CPCA01N63/22A01N63/28C12R2001/465C12R2001/07C12N1/205A01H3/00G16B50/00G16B40/00G16B20/20G16B30/10A01N63/20C12Q1/02C12R2001/01C12N1/00C12Q1/00C12Q1/18
Inventor L·L·金克尔
Owner RGT UNIV OF MINNESOTA
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