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Method for improving content and aromas of tobacco secondary metabolite products

A technology for secondary metabolites and secondary metabolism, which can be used in botanical equipment and methods, chemicals for biological control, horticultural methods, etc. The effect of improving aroma and improving productivity

Inactive Publication Date: 2013-03-27
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since cyclization is a key step in the conversion of lycopene to cyclic carotenoids, although this method can promote the accumulation of acyclic carotenoid lycopene in tobacco leaves, it inhibits and reduces the concentration of cyclic carotenoids. Such as the accumulation of β-carotene (S.Al-Babili. CPTA Modulates Levels of Carotenogenic Proteins and their mRNAs and Affects Carotenoid and ABA Content as well as Chromoplast Structure in Narcissus pseudonarcissus Flowers. Plant Biology, 1999, 1: 607-612)
It is known that lycopene itself has no aroma-enhancing effect, and its cyclization products include cyclic carotenoids such as β-carotene, lutein, cryptoxanthin, etc., which are the important aroma components of tobacco leaves β-damascenone, β-di Precursors of hydrodamascenone, β-ionone, oxidized isophorone, macrostigmatrienone, etc.
It can be seen that inhibiting the cyclization of lycopene will inhibit the formation of cyclic carotenoid derivatives or degradation products of important aroma components in tobacco leaves, which may have a negative impact on tobacco aroma

Method used

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  • Method for improving content and aromas of tobacco secondary metabolite products
  • Method for improving content and aromas of tobacco secondary metabolite products
  • Method for improving content and aromas of tobacco secondary metabolite products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take 0.1 kg of commercially available methyl jasmonate reagent, add 0.2 kg of ethanol to methyl jasmonate, stir to dissolve, then add 0.05 kg of Tween and 965 kg of water, and mix evenly to obtain the tobacco secondary metabolism elicitor solution of the present invention. The concentration of methyl jasmonate is about 100mg / L. One month after transplanting tobacco, spray evenly on the leaf surface, subject to leaf wetness. Ten days after the tobacco flavoring inducer was treated, samples were taken to determine the content of volatile aroma components in tobacco leaves and related indicators. The results showed that the tobacco flavoring inducer increased the glandular hair secretion content of tobacco by 40%, petroleum ether extract by 38%, polyphenol compound content by 48%, and carotenoid content by 32%. The effect of the tobacco flavoring inducer on the content of important volatile aroma components in tobacco leaves is shown in Table 1. It can be seen that the tob...

Embodiment 2

[0038] Take 0.1 kg of commercially available methyl jasmonate reagent, 0.2 kg of salicylic acid, and 0.2 kg of chitosan for use. Mix 0.1 kg of methyl jasmonate and 0.2 kg of salicylic acid, and then dissolve it with a small amount of ethanol, then use Tween as an auxiliary, add water to mix and dissolve thoroughly, and set aside. Add 0.2kg of chitosan to water and stir to dissolve it (add a small amount of dilute acid if necessary). Then mix the above solutions, add water to the total weight of the solution to 1000kg, to obtain the tobacco secondary metabolism elicitor mixed solution of the present invention, wherein the concentration of methyl jasmonate is about 100mg / L, the concentration of salicylic acid is about 200mg / L, and the concentration of chitosan The concentration is about 200mg / L. On the day after the tobacco is topped, spray evenly on the leaf surface of the tobacco plant, subject to the wetness of the leaf surface. 10 days before the tobacco leaves are harveste...

Embodiment 3

[0040] Take 1kg of commercially available methyl jasmonate reagent and 2kg of salicylic acid. After mixing the two, first dissolve them with proper amount of ethanol, and then use Tween as the auxiliary agent. After stirring evenly, add 100 kg of water and mix evenly to obtain the tobacco flavoring inducer of the present invention, wherein the content of methyl jasmonate is about 1% , Salicylic acid content is about 2%. When in use, the tobacco flavoring inducer is diluted 20 to 100 times, and the foliar spray method is used to spray evenly on the upper and lower surfaces of the tobacco leaves 10 days before the tobacco leaves are harvested. The analysis of the tobacco leaves after harvesting and conditioning shows that the tobacco flavoring inducer of the present invention can increase the solanesol content in the tobacco leaves by 30%.

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Abstract

The invention provides a method for improving the content and aromas of tobacco secondary metabolite products. During any tobacco plant growth period or after tobacco harvesting, a leaf surface spraying method, a stem top spraying method or a root-irrigation method is adopted to implement one or more of salicylic acid elicitors and / or jasmonic elicitors on the tobacco plants or the tobaccos so as to induce synthesis of the tobacco secondary metabolite products. The method can not only remarkably increase the content of tobacco essential oil, petroleum ether extract and glandular hair secreta and the like and improve tobacco aroma quality, but also improve yield of some important natural products in the tobacco such as solenesol and provide high-content raw materials for extraction of the natural products. Meanwhile, the method can activate tobacco plant immune system and improve insect resistance, disease resistance and stress resistance of the tobacco plants.

Description

Technical field [0001] The invention relates to the technical field of tobacco production, in particular to a method for improving the content and aroma quality of tobacco secondary metabolites based on the principle of secondary metabolism regulation and a tobacco flavor enhancing inducer. Background technique [0002] In organisms, using primary metabolites as raw materials, through a series of biochemical reactions, some small molecule chemicals that are not necessary for growth and development are further synthesized, which is called secondary metabolism. Plant secondary metabolites can be briefly divided into three categories in terms of chemical structure: phenolic compounds (aromatics), terpenoids, and nitrogen-containing organics; there are many types of products, such as flavonoids, tannins, terpenoids, and steroids Its glycosides, auxins, alkaloids, organic acids, alcohols, ketones, esters, alkanes, etc. The main flavor components in tobacco, such as volatile oil, petr...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/06A01N43/16A01N37/42A01N37/40A01P21/00
Inventor 朱大恒席宇高明杜雷赵高岭
Owner ZHENGZHOU UNIV
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