Half-dry solid state cultivation method used for industrial production of microalgae

A semi-dry solid state, microalgae biological technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as inability to industrially produce microalgae, achieve easy regulation of cell growth state, and loss of light energy transmission The effect of reducing and solving harvest difficulties

Active Publication Date: 2012-03-14
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a semi-dry solid-state culture method for the industrial pro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A plexiglass plate with a length × width of 0.5 m × 0.5 m and a thickness of 0.003 m was wrapped with 5 layers of medical gauze, covered with a layer of analytical filter paper, and then soaked with 1L of BG11 medium. The Scenedesmus cell culture solution was attached to a cellulose acetate filter membrane with a pore size of 0.45 μm by vacuum filtration, and the biomass density was 20 g m -2 . Spread the cellulose acetate filter membrane attached with Scenedesmus cells on top of the analysis filter paper, and place it inside a glass box (length x width x height 0.5m x 0.5m x 0.03m, the material is ordinary 0.003m thick Glass). One side of the glass box (0.5m × 0.03m) is open to facilitate the taking and placing of the culture plate, and the middle parts of the other two opposite sides have circular openings with a diameter of 0.003m, and a silicone tube with an inner diameter of 0.003m is inserted into the opening as a Intake and exhaust ports. The intake air is com...

Embodiment 2

[0035] A setup similar to that in Example 1 was used, but the artificial light source at the top of each layer was removed. Scenedesmus was cultured in one step under outdoor conditions and triglyceride accumulation was induced. Daytime ventilation flow rate 5L min -1 , carbon dioxide concentration 0.5% (V / V); after filling the glass box with pure carbon dioxide at night, stop ventilation. Every 4 hours during the day, the cell population was sprayed with BG11 to moisturize the cells, and the nitrogen concentration of BG11 was reduced to 0 within 5 days. The results showed that after continuous culture for 10 days, the average growth rate of cells in each culture phase was 15 g m -2 d -1 , the cell growth rate per unit area of ​​the culture system is 150g m -2 d -1 , the total lipid content of the cells reached 55% (by dry weight), and the neutral lipid content reached 49.5% (by dry weight). In the same time period, compared with the upright flat photobioreactor (actua...

Embodiment 3

[0037] A white sponge of 1m x 0.4m and a thickness of 0.003m is respectively bonded to both sides of a high-transmittance organic glass plate with a length x width of 1m x 0.4m and a thickness of 0.003m. After diluting Haematococcus pluvialis species with BG11 medium (biomass concentration 0.1g L -1 ) sprayed on the sponge. Fix 15 covered organic plates inoculated with algae solution on a rotating device. The structure of the carousel is similar to a conveyor belt placed vertically. In a steel frame structure with a length × width × height of 1.1m × 1m × 2.2m, two shaft structures with a diameter of 0.2m are arranged along the center line, of which the lower cut surface is 0.6m from the ground, and the upper and lower shaft The wheelbase is 1.3m, and the two shafts are connected with the motor through a transmission mechanism, and they can move in the same direction synchronously. A track is connected to the two axles. The crawler track is composed of 15 pieces of 0.5m×0.2...

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Abstract

The invention provides a half-dry solid-state cultural method for industrial production of microalgae. The method comprises the following steps: firstly, inoculating microalgae cells on a solid material and allowing cell colony to maintain moist by supplementing liquid; then, adding inorganic carbon sources into the cell colony under the condition of illumination; finally, regulating and controlling and metabolism of microalgae cells by controlling parameters of components in wetting liquid, illumination intensity, the concentration of the carbon source and the like so as to realize accumulation of microalgae biomass and/or secondary metabolites. According to the invention, the practice of using considerable water as a supporting medium in the conventional liquid immersion culture method is abandoned, and the volume and weight of a culture system are reduced, thereby thoroughly solving the problem that a microalgae photobioreactor is difficult to become large-sized and has a low space utilization rate due to limit of material strength and reducing cost for equipment and operation; microalgae has high efficiency in utilizing nutrients, luminous energy and carbon sources and is fast in inducing secondary metabolites, thereby substantially improving output of biomass and secondary metabolites per unit land occupation area.

Description

technical field [0001] The invention belongs to the field of industrial production of microalgae and microalgae products, and in particular relates to a semi-dry solid-state culture method for large-scale production of microalgae biomass and secondary metabolites. Background technique [0002] Microalgae refers to aquatic planktonic algae capable of photosynthesis. Some microalgae are rich in protein and can be used as aquatic bait or livestock feed (such as spirulina); more importantly, some microalgae can synthesize a large amount of secondary metabolites, such as oil, carotenoids, polysaccharides, etc. , These substances are often biologically active substances with extremely high economic value, and can be used in functional foods, food additives, pharmaceuticals, bioenergy and other fields. In particular, extracting microalgae oil through large-scale cultivation of microalgae, and then transforming it into biodiesel is considered to be one of the most important ways to...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12C12N11/00
Inventor 刘天中王俊峰张维陈晓琳彭小伟陈昱陈林高莉丽
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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