Mixed bacterial agent for reducing viscosity of thick oil, as well as preparation method and use thereof

A mixed bacterial agent and viscosity-reducing technology is applied in the direction of microorganism-based methods, biochemical equipment and methods, and drilling compositions, etc. It can improve the emulsification viscosity reduction ability, high adaptability, and no secondary pollution.

Inactive Publication Date: 2013-02-13
E TECH ENERGY TECH DEV CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the composition of petroleum is too complex and diverse, it cannot be completely processed by a single oil recovery functional bacterium
Most of the existing viscosity reducers are single strains, which have poor viscosity reduction effect on heavy oil and cannot improve the fluidity of crude oil and other technical problems.

Method used

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  • Mixed bacterial agent for reducing viscosity of thick oil, as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A mixed bacterial agent for viscous reduction of heavy oil, composed of emulsifying crude oil bacteria Xanthomonas sp., medium- and long-chain hydrocarbon degrading bacteria Pseudomonss sp., colloidal asphaltenes Composed of Microbacterium sp. and Bacillus sp., in the mixed bacterial agent, the emulsified crude oil bacteria Xanthomonas spp., medium and long chain hydrocarbon degrading bacteria Pseudomonas spp., colloidal asphaltene degradation The volume ratio of the fermented liquid of the genus Microbacterium and the genus Bacillus is 20-100:40-180:30-100:10-80. The effective number of viable bacteria in the mixed bacterial agent is 10 8 ~10 10 pieces / ml.

Embodiment 2

[0039] A method for preparing a mixed microbial agent for viscous reduction of heavy oil. The preparation process is to combine Xanthomonas sp., Pseudomonss sp., Microbacterium sp. ) and the primary seed liquid of Bacillus sp. were fermented and mixed separately. The specific steps are as follows:

[0040] (1) Source of bacteria

[0041] The strains were domesticated and screened from Bohai Bay produced water with viscous crude oil as the only carbon source, and they were Xanthomonas, Pseudomonas, Microbacterium and Bacillus;

[0042] (2) Preparation of primary seed solution

[0043] The strains obtained in step (1) were respectively activated on peptone yeast extract slant medium for 24h-30h, then transferred to liquid peptone yeast extract medium and activated in a shaker flask for 36-72h, as the primary seed solution. The activation temperature of the shake flask is 28-37°C, and the rotation speed is 100-250r / min.

[0044] Peptone yeast extract slant medium is beef extra...

Embodiment 3

[0049] A method for preparing a mixed bacterial agent for thick oil viscosity reduction, including the preparation of mixed bacterial fermentation broth, Xanthomonas sp., Pseudomonas sp., Microbacterium sp.) and Bacillus (Bacillus sp.), the bacterial fermentation liquid obtained through slant culture, shake flask activation culture and fermentation culture respectively, the specific operation steps are as follows:

[0050] (1) Incline medium

[0051] Prepare beef extract protein aged solid medium, the specific steps are: yeast extract 5g, protein aged 10g, NaCl 10g, distilled water 1000m1, pH 7.2-7.4, agar 1.5%-2%, stir evenly, and sterilize at 121.0°C for 25min. After sterilization, make inclined planes and place them on ultra-clean workbenches. Aseptically inoculate the preserved strains onto the inclined test tube, culture the strains at 30-37°C for 24-36 hours, and wait for the bacterial lawn to cover the inclined surface for later use.

[0052] (2) Preparation of seed s...

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Abstract

The invention relates to a mixed bacterial agent for reducing viscosity of thick oil, as well as a preparation method and a use thereof, belonging to the technical field of crude oil recovery. The mixed bacterial agent for reducing the viscosity of the thick oil comprises xanthomonas, pseudomonas, microbacterium and bacillus, and the volume ratio of fermentation solutions of all bacterial genera is (20-100):(40-180):(30-100):(10-80). The preparation method comprises the following steps: respectively fermenting and mixing first-stage seed solutions of all bacteria: (1) getting strains from sources: extracting the strains out of water of Bohai sea gulf, and taking thick crude oil as a carbon source for acclimation and screening; (2) preparing the first-stage seed solutions: respectively activating on a peptone-yeast slant culture medium and transferring into a liquid peptone-yeast culture medium for shaking and activation; and (3) preparing a fermentation solution of mixed bacteria: respectively transferring the first-stage seed solutions of the strains into a fermentation culture medium for culture, wherein the living bacteria count in the mixed bacteria is 10<8>-10<10> bacteria/ml. The mixed bacterial agent for reducing the viscosity of the thick oil is used for reducing the viscosity of the crude oil. The mixed bacterial agent for reducing the viscosity of the thick oil, disclosed by the invention, has the advantages of high activity, rapid growth, no secondary pollution, good viscosity-reducing effect and the like.

Description

technical field [0001] The invention belongs to the technical field of crude oil recovery, and in particular relates to a mixed microbial agent for viscous reduction of heavy oil, a preparation method and application thereof. Background technique [0002] At present, 60% of the offshore oilfields in the Bohai Bay are heavy oil. Heavy oil is heavy crude oil with high viscosity. The key to the problem of heavy oil production. [0003] According to the characteristics of heavy oil in offshore oilfields, microorganisms are used to emulsify and reduce the viscosity of crude oil with high colloidal asphaltene content. Different microorganisms have different viscosity-reducing rates for various components of petroleum, and it is extremely difficult to reduce the viscosity of high-molecular aromatic hydrocarbons, colloids and asphaltenes. Compared with genetically engineered bacteria, oil recovery functional bacteria screened from nature are not only stable, have strong adaptabili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K8/582C12R1/64C12R1/38C12R1/07C12R1/01
Inventor 胡欣
Owner E TECH ENERGY TECH DEV CORP
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