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Process for preparing immobilization compound microorganism preparation for controlling secondary pollution of river channel bed mud

A compound bacterial agent, secondary pollution technology, applied in chemical instruments and methods, water/sludge/sewage treatment, bacteria, etc., can solve the problems of sediment resuspension, frequent dosing, and high cost, and achieve easy loss, Pollution release reduction, the effect of reducing pollution

Inactive Publication Date: 2005-11-02
GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the measures used to control the secondary pollution of sediment were mainly dredging and dredging. This method is costly, and the reprocessing of sediment is also a complex environmental protection problem; through reoxygenation of water body, sediment pollution can be reduced to a certain extent , but it is easy to cause sediment re-suspension in shallow rivers; adding microbial preparations in rivers, this method has a certain effect on enhancing the self-purification ability of water bodies, but the disadvantage is that microbial preparations are easy to be lost in rivers, frequent dosing, and the cost is too high high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Firstly, the immobilized composite bacterial agent was prepared, and then the application comparison experiment was carried out in the simulated river channel.

[0021] The preparation method of immobilized composite bacterial agent is as follows:

[0022] Use Rhodopseudomonas palustris (Rhodpseudomonas palustris) ZZH-01 strain as the bacterial strain, insert the bacterial strain into the liquid culture medium, and cultivate under anoxic conditions with a light intensity of 1000lx, a temperature of 30°C, and a culture time of 72 hours to obtain a seed culture solution Then press 10% of the inoculum size to access the liquid culture medium for amplified cultivation, under anoxic conditions, light intensity 1000lx light cultivation, temperature 30 ℃, cultivation time 96 hours, obtain bacteria content 20 × 10 8 photosynthetic bacteria fermentation broth per milliliter. The formula of photosynthetic bacteria liquid culture medium is calculated by mass fraction: ammonium ch...

Embodiment 2

[0027] Use Rhodopseudomonas palustris (Rhodpseudomonas palustris) ZZH-02 bacterial strain as the strain, insert the strain into the liquid culture medium, and cultivate under anoxic conditions with an illumination of 2000lx, at a temperature of 25°C, and for a cultivation time of 48 hours to obtain a seed culture solution Then press the inoculum size of 5% by mass fraction to access the liquid culture medium for amplified culture, under anoxic conditions, light intensity 1000lx light culture, temperature 25 ℃, culture time 72 hours, obtain bacteria content 10 × 10 8 photosynthetic bacteria fermentation broth per milliliter. The formula of photosynthetic bacteria liquid culture medium is calculated by mass fraction: ammonium chloride (NH 4 Cl) 0.10%, dipotassium hydrogen phosphate (K 2 HPO 4 )0.02%, sodium acetate (CH 3 COONa) 0.40%, magnesium sulfate (MgSO 4 ·7H 2 O) 0.05%, sodium chloride (NaCl) 0.40%, calcium chloride (CaCl 2 ): 0.02%, yeast extract 0.02%, water 98.99%...

Embodiment 3

[0032] Use Rhodopseudomonas palustris (Rhodpseudomonas palustris) ZZH-03 strain as the bacterial strain, insert the bacterial strain into the liquid medium, and cultivate under the condition of anoxic light with an illumination of 3000 lx, the temperature is 35°C, and the cultivation time is 72 hours to obtain the seed culture solution Then press 15% of the inoculum size to access the liquid culture medium to enlarge the culture, under the anoxic condition, the illuminance is 3000lx light culture, the temperature is 35°C, and the culture time is 96 hours, and the bacteria content is 20 × 10 8 photosynthetic bacteria fermentation broth per milliliter. The formula of photosynthetic bacteria liquid culture medium is calculated by mass fraction: ammonium chloride (NH 4 Cl) 0.12%, dipotassium hydrogen phosphate (K 2 HPO 4 )0.03%, sodium acetate (CH 3 COONa) 0.5%, magnesium sulfate (MgSO 4 ·7H 2 O) 0.07%, sodium chloride (NaCl) 0.5%, calcium chloride (CaCl 2 ): 0.04%, yeast ex...

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Abstract

The invention relates to a method for preparation of immobilized complexion bacterial agent used as controlling the secondary pollution to river bottom mud, which is characterized in that individually prepare photosynthetic bacterium fermenting culture liquid and bacillus fermenting culture liquid, and prepare immobilized complexion bacterial agent in solid fermenting culture medium. The immobilized complexion bacterial agent is not easy to leak after putting into the polluted river, not only exerting the degradation of microbial bacterial agent to contaminant, and also exerting the adsorption and immobilization of zeolitic powder to the contaminant given off by bottom mud, and thus gaining the ends of decreasing the bottom mud pollution to superjacent water. The tests indicate that the pollution deactivation can decreases dramatically with the immobilized complexion bacterial agent developed in the invention.

Description

technical field [0001] The invention relates to a preparation method of an immobilized composite bacterial agent for controlling secondary pollution of river bottom mud. Background technique [0002] Due to the long-term settlement and accumulation of domestic pollutants, the black and smelly bottom mud of urban rivers is silted up. Urban river bottom mud pollution is an important source of black and smelly water bodies. Especially when external source pollution of water is controlled, bottom mud pollution becomes the source of water body pollution. main source. The river sediment contains a large amount of organic substances and nutrients, and has a high oxygen consumption rate. Generally, it is in a strong reducing environment. Secondary pollution of the sediment can easily lead to high levels of harmful substances such as ammonia nitrogen, nitrite, sulfide, and skatole in the water body. It causes "black and smelly" water bodies, affecting urban landscape and residents' ...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 陈繁忠徐文新盛彦清傅家谟
Owner GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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