Treatment method for improving digestion and utilization ratio of broad beans of fish and broad bean fermented material
A treatment method and utilization rate technology, applied in application, fungus, animal feed, etc., can solve the problems of insufficient treatment effect and treatment degree, lack of broad bean content, low growth performance, etc., to improve the growth performance of fish , reduce anti-nutritional factors, improve the effect of utilization
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[0039] 1. Activation of strains and preparation of compound bacterial agents;
[0040] (1) Dip the inoculation loop into glycerol-preserved yeast and Bacillus bacteria liquid, respectively streak onto YPD agar plate (10g yeast powder, 20g peptone, 20g glucose, 16g agar powder, 1000mL water), and incubate at 30°C for 24h;
[0041] (2) Pick a single colony into 100mL YPD liquid medium (10g yeast powder, 20g peptone, 20g glucose, 1000mL water), shake and cultivate overnight at 30°C and 180rpm on a shaking table;
[0042] (3) inoculate into fresh YPD liquid culture medium with 2% inoculum amount, shake and culture at 30° C. 180 rpm for 24 hours to obtain yeast fermentation liquid and bacillus fermentation liquid;
[0043] (4) Mix the yeast fermentation broth and the Bacillus fermentation broth at a volume ratio of 1:1, centrifuge at 5000 rpm at 4°C for 20 min, and discard the supernatant to obtain a composite bacterial agent.
[0044] 2. Preparation of broad beans for fermentatio...
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