Feed additive for relieving liver glycogen accumulation of micropterus salmoides and application of feed additive

Abstract

The invention discloses a feed additive for relieving liver glycogen accumulation of micropterus salmoides and application of the feed additive. The feed additive comprises a compound plant extract and an amino acid mixture, the part ratio of the composite plant extract to the amino acid mixture is (22-27): (50-60); the composite plant extract is prepared from curcumin, resveratrol, chicoric acid, berberine, quercetin, licoflavone, astragalus polysaccharide, zanthoxylin and danshinone; the amino acid mixture is prepared from L-arginine, L-leucine, L-lysine and L-alanine. Preferably, the feed additive also comprises 18-22 parts of coated short-chain fatty acid salt. The components of the feed additive can jointly activate insulin signal channels of the micropterus salmoides, promote glycolysis, inhibit gluconeogenesis, relieve insulin resistance induced by high glucose and reduce liver glycogen accumulation of the micropterus salmoides.

Description

Technical Field

[0001] The present invention relates to the technical field of aquaculture, and specifically relates to a feed additive for alleviating hepatic glycogen accumulation in largemouth bass and its application. Background Art

[0002] Largemouth bass, commonly known as California bass, is an important economic fish species in aquaculture in China. Nutritional physiology research has found that carnivorous fish such as largemouth bass have extremely low utilization and tolerance of sugar. Excessive sugar in the feed will cause excessive accumulation of hepatic glycogen in largemouth bass, leading to problems such as hepatocyte swelling and nuclear offset, causing liver damage, and further endangering its health and growth. In recent years, due to the shortage of fish meal resources, a large amount of application of plant-based protein raw materials, and the specific requirements of floating extruded feed processing for starch content, the content of digestible sugar in the feed often exceeds the sugar tolerance of largemouth bass.

[0003] It has been proven that the insulin signaling pathway plays a key role in the regulation of sugar metabolism in largemouth bass. However, after long-term feeding of high-sugar feed, largemouth bass not only fails to effectively activate the insulin signaling pathway, but also induces insulin resistance, which in turn leads to sugar metabolism imbalance and induces excessive accumulation of hepatic glycogen. Therefore, there is an urgent need to develop a safe and efficient feed additive to activate the insulin signaling pathway, improve the sugar utilization ability of largemouth bass, reduce hepatic glycogen accumulation, and alleviate nutritional hepatobiliary diseases caused by the application of compound feed.

[0004] The patent application with the publication number CN116998593A discloses the application of Lactobacillus plantarum in a preparation for improving the sugar tolerance of fish. The probiotic is Lactobacillus plantarum, which can alleviate the hepatic glycolipid metabolic disorder of largemouth bass caused by high-sugar diet feeding and enhance the antioxidant capacity of its liver by maintaining the homeostasis of the intestinal flora of largemouth bass and regulating the hepatic PPAR signaling pathway and the tricarboxylic acid cycle pathway.

[0005] Most current studies are limited to the research on the sugar utilization ability of largemouth bass by a single additive. Generally speaking, the effect is limited, and there is a lack of a systematic plan for alleviating hepatic glycogen accumulation in largemouth bass. Summary of the Invention

[0006] Aiming at the above problems existing in the prior art, the purpose of the present invention is to provide a composite feed additive for alleviating hepatic glycogen accumulation in largemouth bass and its application in largemouth bass feed.

[0007] To achieve the above purpose, the present invention provides the following technical solutions:

[0008] In a first aspect, the present invention provides a feed additive for alleviating glycogen accumulation in largemouth bass, comprising a compound plant extract and an amino acid mixture; the ratio of the compound plant extract to the amino acid mixture is (22 - 27):(50 - 60);

[0009] The compound plant extract includes curcumin, resveratrol, chicoric acid, berberine, quercetin, liquiritin, astragalus polysaccharide, xanthyletin and tanshinone;

[0010] The amino acid mixture includes L-arginine, L-leucine, L-lysine and L-alanine.

[0011] The feed additive includes a compound plant extract that can activate the expression of the SIRT1 gene, inhibit the expression of the negative regulator PTP1B gene of the insulin signaling pathway, and thus activate the insulin signaling pathway, and an amino acid mixture that can effectively stimulate insulin secretion. They can jointly activate the insulin signaling pathway, promote glycolysis, inhibit gluconeogenesis, alleviate high-sugar-induced insulin resistance, and reduce glycogen accumulation in the liver. Chicoric acid and quercetin are added as main components in the compound plant extract. The addition of chicoric acid can activate AMPK to promote the expression of SIRT1, and thus activate the insulin signaling pathway. In addition to synergistically activating the AMPK / SIRT1 pathway with chicoric acid, the addition of quercetin can also effectively inhibit the expression of PTP1B, and thus activate the insulin signaling pathway.

[0012] Further, the compound plant extract includes curcumin, resveratrol, chicoric acid, berberine, quercetin, liquiritin, astragalus polysaccharide, xanthyletin and tanshinone, and the compounding ratios of each component are 12 - 14 parts, 10 - 12 parts, 6 - 8 parts, 4 - 6 parts, 5 - 7 parts, 3 - 5 parts, 2 - 4 parts, 1 - 3 parts, 1 - 3 parts respectively. The compound plant extract with this ratio can activate the expression of the SIRT1 gene, inhibit the expression of the PTP1B gene, and thus activate the insulin signaling pathway.

[0013] Further, the amino acid mixture includes L-arginine, L-leucine, L-lysine and L-alanine, and the compounding ratios of each component are 8 - 12 parts, 8 - 10 parts, 6 - 8 parts, 5 - 7 parts respectively.

[0014] Further, the feed additive further includes an encapsulated short-chain fatty acid salt, and the compounding ratio of each component is: 22 - 27 parts of the compound plant extract, 50 - 60 parts of the amino acid mixture, and 18 - 22 parts of the short-chain fatty acid salt. Since the short-chain fatty acid salt has a certain volatile odor, it is encapsulated to avoid the volatile odor from affecting the feeding of fish.

[0015] The short-chain fatty acid salt complex, in combination with the compound plant extract and the amino acid mixture, can further activate the insulin signaling pathway, promote glycolysis, inhibit gluconeogenesis, relieve high-glucose-induced insulin resistance, and reduce hepatic glycogen accumulation.

[0016] Further, the short-chain fatty acid salts include sodium diacetate, sodium propionate, and sodium butyrate, and the compounding ratios of each component are 5-8 parts, 3-5 parts, and 2-4 parts respectively.

[0017] Further, the compounding ratio of each component of the feed additive is: 25 parts of the compound plant extract, 55 parts of the amino acid mixture, and 20 parts of the short-chain fatty acid salt.

[0018] Further, the coating component of the short-chain fatty acid salt is zein.

[0019] Further, the zein accounts for 2% of the total mass of the short-chain fatty acid salt complex.

[0020] In a second aspect, the present invention provides an application of a feed additive in relieving hepatic glycogen accumulation in largemouth bass. The feed additive is the feed additive as described above, and its mass accounts for 1.5-2.5% of the basic feed formula of largemouth bass.

[0021] Further, the mass of the feed additive accounts for 2% of the basic feed formula of largemouth bass.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The compound plant extract in the compound feed additive of the present invention introduces chicoric acid and quercetin, which can effectively activate the expression of SIRT1, and at the same time inhibit the negative regulator PTP1B of the insulin signaling pathway, thereby enhancing the activation effect on the insulin signaling pathway, promoting glycolysis, inhibiting gluconeogenesis, and further reducing the hepatic glycogen content.

[0024] The compound plant extract in the compound feed additive of the present invention can activate the expression of the SIRT1 gene and inhibit the expression of the PTP1B gene, thereby indirectly activating the insulin signaling pathway; the amino acid mixture can effectively stimulate insulin secretion and directly activate the insulin signaling pathway. It activates the insulin signaling pathway together with the compound plant extract, promotes glycolysis, inhibits gluconeogenesis, can relieve high-glucose-induced insulin resistance, and reduce hepatic glycogen accumulation. The effect is significantly better than using the compound plant extract, amino acid mixture, and short-chain fatty acid salt complex alone.

[0025] On the basis of this compound feed additive, adding a short-chain fatty acid salt complex can further activate the insulin signaling pathway, promote glycolysis, inhibit gluconeogenesis, reduce blood sugar content, and slow down the accumulation of liver glycogen, thereby improving the ability of largemouth bass to utilize sugar, and the effect is better than that of the plant extract-amino acid complex. Detailed implementation mode

[0026] The following further illustrates the present invention with specific embodiments, but it is not a limitation of the present invention. For the execution order of actions and steps in the devices and methods shown in the claims and the specification, as long as there is no specific explicit order limitation, and as long as the output of the previous process is not used in the subsequent process, it can be implemented in any order.

[0027] The raw material compounds, solvents, etc. involved in the following examples and examples are all commercially available. The detection instruments and detection reagents involved in the following effect examples are all commercially available. The detection methods used are existing technologies that can be retrieved, and the detection environment is normal temperature environment. The basic feed formula for largemouth bass used in the following examples is a common commercial formula, specifically: 40 parts of fish meal, 13 parts of fermented soybean meal, 11 parts of corn protein powder, 5 parts of shrimp powder, 2 parts of blood powder, 3 parts of wheat gluten powder, 1.5 parts of yeast powder, 2.5 parts of phospholipid oil, 5 parts of soybean oil, 1 part of calcium dihydrogen phosphate, 1 part of compound vitamin, 1 part of compound mineral, 8 parts of starch, 3 parts of microcrystalline cellulose, 3 parts of zeolite powder.

[0028] Example 1

[0029] In a feed additive for alleviating liver glycogen accumulation in largemouth bass, there are 25 parts of compound plant extract and 55 parts of amino acid mixture.

[0030] The compound plant extract includes 13 parts of curcumin, 11 parts of resveratrol, 7 parts of chicoric acid, 4 parts of glycyrrhizin, 5 parts of berberine, 6 parts of quercetin, 4 parts of astragalus polysaccharide, 2 parts of xanthyletin, and 2 parts of tanshinone;

[0031] The amino acid mixture includes 10 parts of L-arginine, 9 parts of L-leucine, 7 parts of L-lysine, and 6 parts of L-alanine;

[0032] After the above feed additive is fully mixed, it is added to the basic feed of largemouth bass at a dose of 2.0%.

[0033] Example 2

[0034] In a feed additive for alleviating liver glycogen accumulation in largemouth bass, there are 22 parts of compound plant extract and 50 parts of amino acid mixture.

[0035] The components of the composite plant extract are 12 parts of curcumin, 10 parts of resveratrol, 6 parts of chicoric acid, 3 parts of glycyrrhizin flavonoids, 4 parts of berberine, 5 parts of quercetin, 3 parts of astragalus polysaccharide, 1 part of xanthyletin, and 1 part of tanshinone;

[0036] The components of the amino acid mixture are 8 parts of L-arginine, 8 parts of L-leucine, 6 parts of L-lysine, and 5 parts of L-alanine;

[0037] After the above feed additives are fully mixed, they are added to the basic feed of Micropterus salmoides at a dose of 1.5%.

[0038] Example 3

[0039] A feed additive for alleviating glycogen accumulation in the liver of Micropterus salmoides contains 27 parts of composite plant extract and 60 parts of amino acid mixture;

[0040] The composite plant extract includes 14 parts of curcumin, 12 parts of resveratrol, 8 parts of chicoric acid, 5 parts of glycyrrhizin flavonoids, 6 parts of berberine, 7 parts of quercetin, 5 parts of astragalus polysaccharide, 3 parts of xanthyletin, and 3 parts of tanshinone;

[0041] The amino acid mixture includes 12 parts of L-arginine, 10 parts of L-leucine, 8 parts of L-lysine, and 7 parts of L-alanine;

[0042] After the above feed additives are fully mixed, they are added to the basic feed of Micropterus salmoides at a dose of 2.5%.

[0043] Example 4

[0044] A feed additive for alleviating glycogen accumulation in the liver of Micropterus salmoides contains 25 parts of composite plant extract, 55 parts of amino acid mixture, and 20 parts of coated short-chain fatty acids;

[0045] The composite plant extract includes 13 parts of curcumin, 11 parts of resveratrol, 7 parts of chicoric acid, 4 parts of glycyrrhizin flavonoids, 5 parts of berberine, 6 parts of quercetin, 4 parts of astragalus polysaccharide, 2 parts of xanthyletin, and 2 parts of tanshinone;

[0046] The amino acid mixture includes 10 parts of L-arginine, 9 parts of L-leucine, 7 parts of L-lysine, and 6 parts of L-alanine;

[0047] The short-chain fatty acid salts include 7 parts of sodium diacetate, 4 parts of sodium propionate, and 3 parts of sodium butyrate. After the above short-chain fatty acid salts are fully mixed, they are coated with zein;

[0048] The specific steps of the coating treatment include: preparing a zein solution with a mass fraction of 10% (using ethanol with a mass fraction of 80% as the solvent), and using the fluidized bed process to coat the short-chain fatty acid salt complex.

[0049] After the above feed additive is fully mixed, it is added to the basic feed of Micropterus salmoides at a dose of 2.0%.

[0050] Example 5

[0051] A feed additive for alleviating glycogen accumulation in the liver of Micropterus salmoides contains 22 parts of compound plant extract, 50 parts of amino acid mixture, and 18 parts of coated short-chain fatty acid salts.

[0052] The components of the compound plant extract are 12 parts of curcumin, 10 parts of resveratrol, 6 parts of chicoric acid, 3 parts of liquiritin, 4 parts of berberine, 5 parts of quercetin, 3 parts of astragalus polysaccharide, 1 part of xanthyletin, and 1 part of tanshinone;

[0053] The components of the amino acid mixture are 8 parts of L-arginine, 8 parts of L-leucine, 6 parts of L-lysine, and 5 parts of L-alanine;

[0054] The short-chain fatty acid salts include 5 parts of sodium diacetate, 3 parts of sodium propionate, and 2 parts of sodium butyrate. After the above short-chain fatty acid salts are fully mixed, they are coated with zein;

[0055] After the above feed additive is fully mixed, it is added to the basic feed of Micropterus salmoides at a dose of 1.5%.

[0056] Example 6

[0057] A feed additive for alleviating glycogen accumulation in the liver of Micropterus salmoides contains 27 parts of compound plant extract, 60 parts of amino acid mixture, and 22 parts of coated short-chain fatty acid salts;

[0058] The compound plant extract includes 14 parts of curcumin, 12 parts of resveratrol, 8 parts of chicoric acid, 5 parts of liquiritin, 6 parts of berberine, 7 parts of quercetin, 5 parts of astragalus polysaccharide, 3 parts of xanthyletin, and 3 parts of tanshinone;

[0059] The amino acid mixture includes 12 parts of L-arginine, 10 parts of L-leucine, 8 parts of L-lysine, and 7 parts of L-alanine;

[0060] The short-chain fatty acid salts include 8 parts of sodium diacetate, 5 parts of sodium propionate, and 4 parts of sodium butyrate. After the above short-chain fatty acid salts are fully mixed, they are coated with zein;

[0061] After the above feed additive is fully mixed, it is added to the basic feed of Micropterus salmoides at a dose of 2.5%.

[0062] Control 1

[0063] No feed additive is added to the basic feed.

[0064] Control 2

[0065] Only plant extracts are added to the basal diet, and the specific ratio refers to Example 1. Replace the amino acid mixture with the non-essential amino acid L-glycine. Other conditions remain unchanged.

[0066] Control Example 3

[0067] Only plant extracts are added to the basal diet, and the specific ratio refers to Example 1, but it does not contain chicoric acid and quercetin. Replace the amino acid mixture with the non-essential amino acid L-glycine. Other conditions remain unchanged.

[0068] Control Example 4

[0069] Only the amino acid mixture is added to the basal diet. The specific ratio refers to Example 1. Replace the plant extract with microcrystalline cellulose. Other conditions remain unchanged.

[0070] Control Example 5

[0071] Only the coated short-chain fatty acid complex is added to the basal diet, and the specific ratio and coating treatment refer to Example 4. Replace the plant extract with microcrystalline cellulose and replace the amino acid mixture with the non-essential amino acid L-glycine. Other conditions remain unchanged.

[0072] Control Example 6

[0073] Add a complex plant extract-amino acid mixture to the basal diet, and the specific ratio refers to Example 1, but the complex plant extract does not contain chicoric acid and quercetin. Other conditions remain unchanged.

[0074] Control Example 7

[0075] Add a mixture of complex plant extract-short-chain fatty acid salt complex to the basal diet, and the specific ratio refers to Example 4. Replace the amino acid mixture with the non-essential amino acid L-glycine. Other conditions remain unchanged.

[0076] Control Example 8

[0077] Add a mixture of amino acid mixture-short-chain fatty acid salt complex to the basal diet, and the specific ratio refers to Example 1. Replace the plant extract with microcrystalline cellulose. Other conditions remain unchanged.

[0078] As described above, 12 compound feeds of Examples 1 to 6 and Comparative Examples 1 - 6 were formulated. Juvenile largemouth bass with an initial body weight of 9.81 ± 0.20 g were used as the research subjects, and the above feeds were used for a 10 - week feeding experiment respectively. Three replicates were set for each treatment, and they were fed twice a day to satiation (at 7:30 and 17:00). After the feeding experiment ended, the weight and quantity of the fish in each barrel were recorded to characterize the changes in growth performance and survival rate; the liver and viscera were separated for calculating the hepatosomatic index and viscerosomatic index; blood was drawn and centrifuged to separate serum for measuring blood glucose and insulin contents; the liver was separated for measuring glycogen content, antioxidant enzyme activity, and gene expression.

[0079] 1. Growth performance and body shape index

[0080] The effects of different additives on the growth performance and body shape index of largemouth bass are shown in Table 1. The results show that the survival rate in each group was 100%. However, compared with Comparative Example 1, the individual addition of compound plant extracts, amino acid mixture, and short - chain fatty acid salts had a certain improvement effect on growth. Among them, the effect of Example 1 was better than their individual addition (Comparative Examples 2 to 6) and other compound additions (Comparative Examples 7 and 8). The effect of Example 4 was the most significant. At the same time, the individual or compound addition of the above additives significantly reduced the hepatosomatic index and viscerosomatic index of largemouth bass. Among them, the hepatosomatic index and viscerosomatic index of largemouth bass in Example 4 were significantly lower than those in other groups. In addition, compared with the corresponding addition groups (Comparative Example 2 and Example 1), the compound plant additives without chicoric acid and quercetin (Comparative Examples 3 and 6) would reduce the growth performance to a certain extent and affect their effect of reducing the hepatosomatic index and viscerosomatic index. This shows that the feed additive of compound plant extracts combined with amino acid mixture, especially the compound addition of chicoric acid and quercetin, can significantly reduce the hepatosomatic index, and the simultaneous compound addition of short - chain fatty acid salts can effectively improve the growth performance of largemouth bass.

[0081] Table 1. Effects of additive application on the growth performance and body shape indexes of largemouth bass

[0082]

[0083]

[0084] Note: Different superscript letters indicate significant differences (P < 0.05)

[0085] 2. Hepatic glycogen content and serum glucose and insulin contents

[0086] The effects of different additive applications on the growth performance and body shape index of Micropterus salmoides are shown in Table 2. The results show that, compared with Comparative Example 1, the individual addition of compound plant extracts, amino acid mixtures, and short-chain fatty acid salts (Comparative Examples 2 to 6) reduced the contents of blood glucose and liver glycogen. The effect of Example 1 in reducing the contents of blood glucose and liver glycogen was better than that of their individual addition and other compound additions (Comparative Examples 7 and 8). The effect of Example 4 was the most significant. In addition, the application of additives significantly increased the content of serum insulin, and its effect was the best in Example 4. Compared with the corresponding addition groups (Comparative Example 2 and Example 1), the compound plant additives without chicoric acid and quercetin (Comparative Examples 3 and 6) would, to a certain extent, affect their effects of reducing blood glucose and liver glycogen and promoting insulin secretion. This indicates that the feed additive obtained by compounding compound plant extracts with amino acid mixtures, especially the compound addition of chicoric acid and quercetin, can significantly reduce the liver glycogen accumulation and blood glucose content of Micropterus salmoides, which may be related to the activation of the insulin signaling pathway. At the same time, compounding with short-chain fatty acid salts can achieve better effects.

[0087] Table 2. Effects of additive applications on liver glycogen accumulation, serum glucose, and insulin contents of Micropterus salmoides

[0088]

[0089]

[0090] Note: Different superscript letters indicate significant differences (P<0.05)

[0091] 3. Antioxidant capacity

[0092] Generally, glycogen accumulation can cause liver damage and a decrease in antioxidant capacity. The effects of different additive applications on the antioxidant capacity of Micropterus salmoides are shown in Table 3 in detail. The results show that the individual addition of compound plant extracts, amino acid mixtures, and short-chain fatty acid salts (Comparative Examples 2 to 6) can reduce the content of malondialdehyde. The effect of Example 1 in reducing the content of malondialdehyde was better than that of their individual addition and the compound addition of compound plant extracts and short-chain fatty acid salts (Comparative Example 7), but inferior to the compound addition of amino acid mixtures and short-chain fatty acid salts (Comparative Example 8). Therefore, amino acid mixtures can effectively reduce the content of malondialdehyde, and the compound effect with short-chain fatty acid salts is better. The effect of Example 4 in reducing the content of malondialdehyde, significantly increasing the total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and catalase (CAT) activities was the best. At the same time, the compound plant additive containing chicoric acid and quercetin can further improve the antioxidant capacity.

[0093] Table 3. Effects of additive applications on the antioxidant capacity of Micropterus salmoides

[0094]

[0095] Note: Different superscript letters indicate significant differences (P<0.05).

[0096] 4. Expression of genes related to the SIRT1 / PTP1B pathway and insulin signaling pathway

[0097] The effects of different additives on the expression of key genes in the SIRT1 / PTP1B pathway and insulin signaling pathway of Micropterus salmoides are shown in Table 3. From the results, the individual addition of compound plant extracts, amino acid mixtures, and short-chain fatty acid salts (Comparative Examples 2 to 6) can promote the expression of the SIRT1 gene and inhibit the expression of PTP1B, a negative regulator of the insulin signaling pathway. The effect of Example 1 is better than their individual addition and other compound additions (Comparative Examples 7 and 8). The effect of Example 4 is the most significant. At the same time, the individual or compound addition of additives significantly promotes the expression of IR, IRS, and AKT1 in the insulin signaling pathway, and the effect is optimal in Example 4. The addition of chicoric acid and quercetin to the compound plant additive has a certain promoting effect on the activation of the insulin signaling pathway. This shows that the feed additive composed of the compound plant extract and amino acid mixture can significantly activate the insulin signaling pathway of Micropterus salmoides, thereby slowing down hepatic glycogen accumulation and maintaining the health of the body. The simultaneous compound addition of short-chain fatty acid salts can achieve better results.

[0098] Table 4. Effects of additive application on the expression of genes related to the SIRT1 / PTP1B pathway and insulin signaling pathway of Micropterus salmoides

[0099] SIRT1 PTP1B IR IRS AKT1 Comparative Example 1 <![CDATA[1.01±0.03 e > <![CDATA[1.00±0.07 a > <![CDATA[1.02±0.02 d > <![CDATA[1.00±0.10 e > <![CDATA[1.00±0.03 e > Comparative Example 2 <![CDATA[1.51±0.22 b > <![CDATA[0.76±0.04 bc > <![CDATA[1.41±0.04 c > <![CDATA[1.42±0.03 c > <![CDATA[1.57±0.03 c > Comparative Example 3 <![CDATA[1.21±0.04 d > <![CDATA[0.86±0.03 b > <![CDATA[1.13±0.05 d > <![CDATA[1.25±0.18 d > <![CDATA[1.28±0.02 d > Comparative Example 3 <![CDATA[1.21±0.03 d > <![CDATA[0.85±0.02 b > <![CDATA[1.36±0.03 c > <![CDATA[1.36±0.02 c > <![CDATA[1.52±0.04 c > Comparative Example 4 <![CDATA[1.28±0.02 d > <![CDATA[0.89±0.03 b > <![CDATA[1.39±0.02 c > <![CDATA[1.40±0.03 c > <![CDATA[1.49±0.07 c > Comparative Example 6 <![CDATA[1.56±0.15 c > <![CDATA[0.83±0.01 b > <![CDATA[1.45±0.02 c > <![CDATA[1.53±0.04 bc > <![CDATA[1.56±0.04 c <!-- 7 -->]]> Comparative Example 5 <![CDATA[1.85±0.23 b > <![CDATA[0.74±0.03 c > <![CDATA[1.69±0.07 b > <![CDATA[1.58±0.02 b > <![CDATA[1.76±0.05 b > Comparative Example 6 <![CDATA[1.31±0.11 d > <![CDATA[0.80±0.06 bc > <![CDATA[1.72±0.04 b > <![CDATA[1.67±0.03 b > <![CDATA[1.79±0.04 b > Example 1 <![CDATA[1.81±0.12 b > 0.70±0.02c 1.75±0.11b <![CDATA[1.69±0.06 b > <![CDATA[1.85±0.08 b > Example 4 <![CDATA[2.05±0.12 a > <![CDATA[0.62±0.16 d > <![CDATA[1.96±0.02 a > <![CDATA[1.84±0.03 a > <![CDATA[2.06±0.09 a >

[0100] Note: Different superscript letters indicate significant differences (P<0.05).

[0101] The effects of the feed additives in Examples 2 and 3 on alleviating hepatic glycogen accumulation in Micropterus salmoides are similar to those in Example 1, and the effects of the feed additives in Examples 5 and 6 on alleviating hepatic glycogen accumulation in Micropterus salmoides are similar to those in Example 4, which will not be elaborated here.

[0102] From the description of the above preparation method and test results, it can be found that:

[0103] The feed additive provided by the present invention can largely activate the insulin signaling pathway of Micropterus salmoides, promote glycolysis, inhibit gluconeogenesis, relieve insulin resistance induced by high sugar, reduce hepatic glycogen accumulation, and promote the healthy growth of Micropterus salmoides.

[0104] Those skilled in the art should understand that those skilled in the art can achieve variation examples in combination with the prior art and the above embodiments, which will not be elaborated here. Such variation examples do not affect the essence of the present invention and will not be elaborated here.

[0105] The preferred embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the above specific embodiments, and the devices and structures not described in detail therein should be understood to be implemented in a common manner in the art; any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make many possible changes and modifications to the technical solution of the present invention by using the methods and technical contents disclosed above, or modify it into an equivalent embodiment with equivalent changes, which does not affect the essence of the present invention. Therefore, any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A feed additive for alleviating liver glycogen accumulation in largemouth bass, characterized in that: It comprises a compound plant extract and an amino acid mixture; the ratio of the compound plant extract to the amino acid mixture is (22 - 27):(50 - 60); The compound plant extract includes curcumin, resveratrol, chicoric acid, berberine, quercetin, liquiritin, astragalus polysaccharide, xanthyletin and tanshinone; The amino acid mixture includes L-arginine, L-leucine, L-lysine and L-alanine.

2. The feed additive for alleviating glycogen accumulation in largemouth bass according to claim 1, wherein The compound plant extract includes curcumin, resveratrol, chicoric acid, berberine, quercetin, liquiritin, astragalus polysaccharide, xanthyletin and tanshinone, and the compounding ratios of each component are 12 - 14 parts, 10 - 12 parts, 6 - 8 parts, 4 - 6 parts, 5 - 7 parts, 3 - 5 parts, 2 - 4 parts, 1 - 3 parts, 1 - 3 parts respectively.

3. The feed additive for alleviating glycogen accumulation in the liver of Micropterus salmoides according to claim 2, characterized in that, The amino acid mixture includes L-arginine, L-leucine, L-lysine and L-alanine, and the compounding ratios of each component are 8 - 12 parts, 8 - 10 parts, 6 - 8 parts, 5 - 7 parts respectively.

4. A feed additive for alleviating liver glycogen accumulation in largemouth bass according to claim 1, characterized in that The feed additive further includes an encapsulated short-chain fatty acid salt, and the compounding ratio of each component is: 22 - 27 parts of the compound plant extract, 50 - 60 parts of the amino acid mixture, and 18 - 22 parts of the short-chain fatty acid salt.

5. A feed additive for alleviating hepatic glycogen accumulation in Micropterus salmoides according to claim 4, characterized in that, The short-chain fatty acid salt includes sodium diacetate, sodium propionate and sodium butyrate, and the compounding ratios of each component are 5 - 8 parts, 3 - 5 parts, 2 - 4 parts respectively.

6. A feed additive for alleviating glycogen accumulation in largemouth bass according to claim 4, characterized in that, The compounding ratio of each component of the feed additive is: 25 parts of the compound plant extract, 55 parts of the amino acid mixture, and 20 parts of the short-chain fatty acid salt.

7. A feed additive for alleviating glycogen accumulation in largemouth bass according to claim 4 or 5, characterized in that, The coating component of the short-chain fatty acid salt is zein.

8. A feed additive for alleviating glycogen accumulation in largemouth bass according to claim 7, characterized in that, The zein accounts for 2% of the total mass of the short-chain fatty acid salt complex.

9. Use of a feed additive in alleviating hepatic glycogen accumulation in largemouth bass, characterized in that, The feed additive is the feed additive according to any one of claims 1 to 8, and its mass accounts for 1.5 - 2.5% of the basic feed formula of largemouth bass.

10. Use of a feed additive according to claim 9 in alleviating glycogen accumulation in the liver of Micropterus salmoides, characterized in that, The mass of the feed additive accounts for 2% of the basic feed formula of largemouth bass.

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