31results about How to "Solve the problem of high production cost" patented technology

The invention discloses an incision beta-1,3-glucanase coding gene from cellulosimicrobium cellulans, as well as enzyme, a preparation method and application of the incision beta-1,3-glucanase coding gene. The incision beta-1,3-glucanase coding gene is cloned to an escherichia coli expression vector through a technical method of gene engineering, so that an escherichia coli recombination strain capable of realizing heterologous expression of the enzyme can be obtained; incision beta-1,3-glucanase prepared through the heterologous expression of the strain can directly and efficiently degrade solid Curdlan. The invention further provides an antibiological inoculant, which is the incision beta-1,3-glucanase, for preventing and treating animal and plant diseases; the antibiological inoculant belongs to the field of biological prevention and treatment, and is expected to be novel biological pesticide. The incision beta-1,3-glucanase provided by the invention can be widely applied to the field of agriculture, food, feed additives, medicines, glucooligosaccharides preparation and the like.

The invention discloses an eucalyptus routing fiber board and a manufacturing method thereof. The method uses eucalyptus wane as a main raw material and comprises the following steps: chip preparation, wood chip steaming, fiber preparation, wax applying, glue applying, molding pre-pressing and hot-pressing, and therefore obtaining the routing fiber board. The routing fiber board per cubic meter comprises the following raw materials by weight: 1200kg of wane chips, 4.0-4.5kg of paraffin wax, 205-210kg of an adhesive and 2.0-3.0kg of a steaming auxiliary agent. The method prepares the eucalyptus routing fiber board by using fast-growing eucalyptus wane with the poor quality, solves the problems of deficiency of high-quality wood and high production cost of the routing fiber board, improves the utilization value of the logging residues of fast-growing eucalyptus wane man-made forest, increases the income of farmers and the economic benefit of enterprises, realizes the wish that high-end products are made of low-end raw materials, and has good economic and social benefits.

The invention discloses a k-carrageenase gene derived from Pedrobacter hainanensis Q-13, a preparation method of its enzyme and an application thereof, a genetic engineering technical method is used to clone the gene of the k-carrageenase to an E. coli expression vector to obtain a recombinant strain of Escherichia coli which can heterologously express the enzyme. The k-carrageenase prepared by heterogenous expression of the strain can efficiently degrade k-carrageenan. The k-carrageenase provided by the invention can be widely used in the fields of agriculture, food, feed additives, medicine,cosmetics and carrageenan oligosaccharide preparation.

The invention discloses a method for producing baohuoside I through herba epimdii. The method comprises the following steps of: S1. expanding propagating and diluting saccharomyces cerevisiae in culture medium; S2. mixing and evenly stirring culture solution and herba epimdii, and carrying out close and static anaerobic fermentation in a fermentation tank; S3. when the herba epimdii is entirely hydrolyzed, extracting fermenting raw materials with an extraction solvent; S4. washing with extract dichloromethane, and extracting ethyl acetate; and S5. filtering ethyl acetate through an alumina column, then carrying out silica gel adsorption column chromatography to obtain pure baohuoside I. The method has the beneficial effects of being simple and convenient to operate, easy to implement, high in efficiency, fast, low in cost, high in yield, pollution-free by adopting microbial solid-state anaerobic fermentation to hydrolyze herba epimdii-containing raw materials, thus being suitable for large-scale industrial production.

The invention discloses an endo-β-1,3-glucanase coding gene derived from Cellulosimerbium cellsulans and its enzyme, preparation method and application. The gene of the endo-β-1,3-glucanase is cloned into an Escherichia coli expression vector to obtain a recombinant E. coli strain capable of expressing the enzyme heterologously, and the endo-β-1,3-glucanase prepared by heterologous expression of the strain is obtained. 3‑glucanase, which can directly and efficiently degrade solid Curdlan (codran polysaccharide). The invention also provides a biocontrol agent for preventing and controlling animal and plant diseases—endo-β-1,3-glucanase, which belongs to the field of biological control and is expected to become a new type of biological pesticide. The endo-β-1,3-glucanase provided by the invention can be widely used in the fields of agriculture, food, feed additive, medicine, glucooligosaccharide preparation and the like.

The invention belongs to the technical field of water pumps, and discloses a pressing die and a pressing method for a vinylidene fluoride impeller with a movable ring. The invention aims to solve theproblem in the production of the vinylidene fluoride impeller with the movable ring in the prior art. The pressing die comprises a bottom die, a core rod, impeller plug sheets, a material barrel, an upper die and the like. The pressing method comprises the following steps that a feeding tray is selected, vinylidene fluoride granules are added into the feeding tray for heating, the die is processedand assembled, the impeller movable ring sleeves the core rod of the die, and by the steps of heating the die and adding materials and pressing and the like, the vinylidene fluoride impeller with themovable ring is produced. The pressing die is suitable for single-piece small-batch production, the die investment is small, the cost is low, and the problem that the vinylidene fluoride production cost is high is effectively solved. According to the pressing die, the impeller movable ring and the impeller are formed in one step, additional bonding is not needed, and the reliability of the movable ring is improved, the time is saved, and the productivity is improved. The pressing die has the advantages that the production method is simple, and the popularization of the vinylidene fluoride impeller is facilitated.

The invention discloses immobilized cephalosporin C acylase and a preparation method thereof. The immobilized cephalosporin C acylase comprises ES-1 resin and cephalosporin C acylase fixed on the ES-1 resin. The preparation method comprises steps as follows: the ES-1 resin and the cephalosporin C acylase are dissolved in a phosphate buffer solution with the concentration being 0.5-1.0 mol / L and the pH being 7.0-10.5 and immobilized at the temperature of 15-35 DEG C for 8-20 h. Compared with the prior art, the provided method for preparing the immobilized cephalosporin C acylase is short in immobilizing time and high in enzyme activity recovery rate, the problem of high production cost in the prior art is solved, and the method is more suitable for industrial production.

The invention discloses a water-borne ultrathin type fireproof heat insulating material and a preparation method thereof. The water-borne ultrathin type fireproof heat insulating material comprises the following components in percentage by weight: 20-40% of puffed basalt particles, 15-30% of water, 35-60% of composite resin, 1.5-3% of a stabilizer, 0.5-1.8% of a dispersant, 0.2-0.5% of a leveling agent, 0.2-0.5% of a thickening agent, 0.2-0.5% of a defoaming agent and 1.5-3% of a heat insulating material MAS. The water-borne ultrathin type fireproof heat insulating material disclosed by the invention is green and environment-friendly, does not release formaldehyde, benzene and other harmful gases, and is stable in performance and high in fireproof heat insulating quality. The water-borne ultrathin type fireproof heat insulating material is simple in preparation process and wide in application range, the problem of high manufacturing cost of EPS, XPS and cement foamed heat insulating plates is solved, meanwhile the working strength and stress of constructors are relieved, the construction difficulty during measuring of special shaped structural parts, cutting of plates and the like by the constructers is reduced, and the construction cost and constriction difficulty are reduced at the same time.

The invention belongs to the technical field of preparation of basic lithium iron phosphate, and in particular relates to a preparation method of basic lithium iron phosphate composite material. The invention comprises the following steps: 1) adding excess iron powder into dilute sulfuric acid to obtain a ferrous sulfate solution; 2) filtering the ferrous sulfate solution prepared in step 1), adding phosphoric acid solution to the filtered solution, and then Adding solid lithium hydroxide and hydrogen peroxide; 3) heating step 2) to obtain a mixed solution, making it react at 68-85° C. to prepare basic lithium iron phosphate. The present invention uses ferrous sulfate as an iron source and adds hydrogen peroxide as a catalyst, so that low-temperature synthesis of basic lithium iron phosphate can be well realized.

The invention discloses an endo-cellulase gene derived from Paenibacillus polymyxa and the preparation method and application of the enzyme, that is, the gene of the endo-cellulase is cloned by using the technical method of genetic engineering On the expression vector of Escherichia coli, a recombinant strain of Escherichia coli capable of heterologously expressing the enzyme is obtained, and the endocellulase prepared by the heterologous expression of the strain can efficiently degrade konjac polysaccharide (also known as konjac glucomannan). The endo-cellulase provided by the invention can be widely used in the fields of agriculture, food, feed additive, medicine, glucomannan oligosaccharide preparation and the like.

The invention discloses a preparation method of a molecular sieve, its application and a waste water utilization method. The method comprises the following steps: (1) uniformly mixing raw materials in water to obtain a mixture; (2) placing the mixture in a hydrothermal reaction kettle for crystallization, and after the crystallization is completed, solid-liquid separation to obtain solid products and mother liquor wastewater, Collecting the mother liquor wastewater; (3) washing, drying and calcining the solid product to obtain a molecular sieve; or adding the solid product to an ion exchange liquid containing copper ions for ion exchange, and then performing solid-liquid separation, washing, drying and calcining in sequence Molecular sieves are obtained after treatment; (4) repeating step (1), while recycling the mother liquor wastewater collected in step (2) and adding it to step (1), so that the raw material in step (1) comes from the mother liquor wastewater. The invention solves the technical problems of serious energy consumption and high production cost in wastewater treatment.

A vertical cavity surface emitting laser based on van der Waals epitaxy and its manufacturing method. In the above manufacturing method, making a GaN-based active region structure includes: covering a single layer of two-dimensional material on a first substrate; The GaN-based active region structure is epitaxially grown on the first substrate of the dimensional material. The GaN-based active region structure includes from bottom to top: an n-type GaN layer, an active region, an electron blocking layer, a p-type GaN layer and a p+GaN layer, and the p+GaN layer serves as the first ohmic contact layer. A first DBR structure is formed on the second substrate. The GaN-based active region structure is peeled off from the first substrate. The stripped GaN-based active region structure is bonded to the first distributed Bragg reflector structure on the second substrate. A second DBR structure is formed over the first ohmic contact layer of the bonded structure. A vertical-cavity surface-emitting laser with low threshold current density and high gain is formed.

The invention relates to a waterproof breathable film installed on a breathable hole of a device. The waterproof breathable film comprises a base body and a waterproof film body, the base body is provided with a first installation portion matched with the breathable hole, the first installation portion is arranged on the outer wall of the breathable hole in a sleeved mode, the top of the first installation portion is fixedly connected with the waterproof film body closing the breathable hole, and the waterproof film body is higher than a drainage channel in the device but lower than an outer shell of the device. The waterproof film body is arranged between the drainage channel and the outer shell, so that the service life of the waterproof film body is prolonged; water on the outer shell of the device is drained through the drainage channel, the water is prevented from covering the waterproof film body and causing efficacy losing of the waterproof film body, the waterproof film body is lower than the outer shell, the function of protecting the waterproof film body is achieved, and the waterproof film body is prevented from being damaged by being touched.

The invention discloses a method for producing baohuoside I through herba epimdii. The method comprises the following steps of: S1. expanding propagating and diluting saccharomyces cerevisiae in culture medium; S2. mixing and evenly stirring culture solution and herba epimdii, and carrying out close and static anaerobic fermentation in a fermentation tank; S3. when the herba epimdii is entirely hydrolyzed, extracting fermenting raw materials with an extraction solvent; S4. washing with extract dichloromethane, and extracting ethyl acetate; and S5. filtering ethyl acetate through an alumina column, then carrying out silica gel adsorption column chromatography to obtain pure baohuoside I. The method has the beneficial effects of being simple and convenient to operate, easy to implement, high in efficiency, fast, low in cost, high in yield, pollution-free by adopting microbial solid-state anaerobic fermentation to hydrolyze herba epimdii-containing raw materials, thus being suitable for large-scale industrial production.

The invention discloses a preparation method and application of internally tangent beta-1,4-glucanase gene derived from paenibacilluspolymyxa and its enzyme. The preparation method is a technique method utilizing genetic engineering. The internally tangent beta-1,4-glucanase gene is cloned to an escherichia coli expression vector to obtain an escherichia coli recombination strain which can heterologously express the enzyme, and the strain heterologously expresses the prepared internally tangent beta-1,4-glucanase and can efficiently degrade konjac glucomannan (also known as konjac glucomanna).The prepared internally tangent beta-1,4-glucanase can be widely applied the fields of preparation of agriculture, foods, feed additives, medicine, oligo-glucomannan and the like.

The invention relates to hydroxypropyl starch and the manufacture method. It is made from starch 1 portion, propylene oxide 0.2-0.8 portion, fatty alcohol polyethenoxy ether 0.06-0.15 portion, NaOH 0.03-0.06 portion, and water 0.12-0.3 portion. The method could obviously improve the reacting speed, and the product could meet the demand of paper industry without neutralization.

The invention discloses an endo-β-1,4-mannanase gene derived from Paenibacillus polymyxa and the preparation method and application of the enzyme. Cloning the gene of cutting β‑1,4‑mannanase into the expression vector of Escherichia coli to obtain a recombinant strain of Escherichia coli that can express the enzyme heterologously Glycanase can efficiently degrade konjac polysaccharides (also known as konjac glucomannan). The endo-β-1,4-mannanase provided by the invention can be widely used in the fields of agriculture, food, feed additive, medicine, glucomannan oligosaccharide preparation and the like.

The invention discloses a method for breeding a green pagoda cauliflower hybrid. In the method, the hybrid progeny of pagoda cauliflower and green cauliflower is obtained through microspore culture or a DH line or a self-bred pure line obtained through traditional breeding. As the male parent, the ogura cytoplasmic male sterile line of common pagoda cauliflower obtained by multi-generation backcrossing is used as the female parent, and a new generation of green pagoda cauliflower hybrid is obtained through crossing. The hybrid has excellent comprehensive resistance, is more cold-tolerant, has green curds, and has better ornamental value.

The invention discloses a breeding method of a green pagoda cauliflower hybrid. A green pagoda cauliflower first-filial generation new variety is obtained by subjecting a pagoda cauliflower and greencauliflower hybrid filial generation to microspore culture or taking conventional breeding selfing homozygous DH line or selfing pure line as a male parent and common pagoda cauliflower ogura cytoplasm male sterile line obtained by multi-generation backcross breeding as a female parent by means of hybridization. The hybrid is excellent in comprehensive resistance, resistant to cold and green in flower ball, thereby having high ornamental value.