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Method for culturing schizochytrium limacinum by utilizing vinasse treating fluid

A technology of Schizochytrium and treatment liquid, which is applied in the field of microbial fermentation production, can solve the problems of high cost, achieve the effects of solving environmental problems, promoting growth and DHA accumulation, and reducing costs

Pending Publication Date: 2022-04-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem of high cost of producing DHA by using Schizochytrium in the prior art, the present invention provides a low-cost, high-yield method for producing DHA by fermentation of Schizochytrium

Method used

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  • Method for culturing schizochytrium limacinum by utilizing vinasse treating fluid
  • Method for culturing schizochytrium limacinum by utilizing vinasse treating fluid
  • Method for culturing schizochytrium limacinum by utilizing vinasse treating fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: Preparation of distiller's grains treatment liquid

[0063] Specific steps are as follows:

[0064] 1. Preparation of distiller's grain treatment liquid

[0065] (1) fresh distiller's grains are dried to make the water content 13%;

[0066] (2) Add water to the distiller's grains obtained in step (1) and soak for 4 hours to obtain a distiller's grains solution; wherein the mass ratio of distiller's grains to water is 1:10;

[0067] (3) Heat the distiller's grains solution obtained in step (2) to boil, and keep it for 1-5 minutes, then filter it with a 200-mesh sieve to obtain the filtrate, centrifuge the filtrate at 5000rpm for 3 minutes, and use a fast filter paper to filter the supernatant The filtered distiller's grains is the distiller's grains treatment liquid.

[0068] 2. Detection of distiller's grain treatment liquid:

[0069] The content of soluble sugar in the distiller's grain treatment liquid was detected by DNS spectrophotometry, and the r...

Embodiment 2

[0075] Example 2: Preparation of fermentation medium containing distiller's grains treatment liquid

[0076] Specific steps are as follows:

[0077] The wine tank treatment solution prepared in Example 1 and deionized water were prepared into a mixed solution in a ratio of 7:3, and the final concentrations were added to the mixed solution: 70g / L glucose, 17.5g / L sea crystal, 20g / L L-sodium glutamate, monohydrate, 2.5g / L potassium dihydrogen phosphate, 7.2g / L magnesium sulfate, 12.8g / L sodium sulfate, 0.4g / L calcium chloride, 0.1g / L VB 1 , 0.1g / L of VB 6 , VB of 0.001g / L 12 , to prepare the fermentation medium.

Embodiment 3

[0078] Example 3: Preparation of algae oil by fermentation of Schizochytrium

[0079] The method for cultivating Schizochytrium by using distiller's grains treatment liquid is a process for fermenting and producing Schizochytrium with culture medium prepared by distiller's grains treatment liquid, using the distiller's grains treatment liquid as a source of nitrogen source for fermentation production, comprising the following steps:

[0080] Seed media involved in the following experiments:

[0081] Glucose 30g / L, tryptone 10g / L, yeast powder 5g / L, sea crystal 15g / L, VB 1 0.05g / L, VB 6 0.05g / L, VB 12 0.0005g / L. Sterilize at 115°C for 20 minutes.

[0082] (1) Cell activation

[0083] Pipette 500 μL of the glycerol tube strain Schizochytrium ATCC20888 stored in a -80°C refrigerator into the seed medium, at 28°C, 200r·min -1 Cultivate for 2 days under the same conditions to obtain culture medium. Dilute the culture medium with water to OD 540 If it is 0.6-0.7, take 100 ...

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Abstract

The invention discloses a method for culturing schizochytrium limacinum by using a vinasse treating fluid, and belongs to the field of microbial fermentation production. According to the method for culturing schizochytrium limacinum by using the vinasse treating fluid, fermentation production is performed by taking the vinasse treating fluid as a main nitrogen source, dry schizochytrium limacinum powder is prepared by soaking, boiling, filtering and centrifuging vinasse, preparing a fermentation culture medium, setting fermentation conditions, collecting and drying schizochytrium limacinum, and algae oil is extracted. The method is a brand new process for fermentation production of schizochytrium limacinum by taking the vinasse treating fluid as a main nitrogen source, and the vinasse treating fluid is used for replacing high-price tryptone and yeast powder in a traditional culture medium, so that the cost of fermentation production is reduced, growth of schizochytrium limacinum and accumulation of DHA are promoted, and the yield of the schizochytrium limacinum is increased. Meanwhile, the environmental problem caused by stacking of the waste vinasse in the liquor factory is effectively solved; the problem of high production cost in the prior art is well solved, and high-value utilization of the vinasse is realized.

Description

technical field [0001] The invention relates to a method for cultivating Schizochytrium by using distiller's grains treatment liquid, and belongs to the field of microbial fermentation production. Background technique [0002] Liquor distiller's grains are a by-product of the liquor production process. In the liquor production process, the starch in the grain is mainly consumed, and the remaining protein, fat, and bacterial protein involved in fermentation are all left in the distiller's grains. Therefore, the crude protein in the distiller's grains and higher crude fat content. Directly discarding distiller's grains is not only a waste of resources, but also causes environmental pollution. At present, the most common way to deal with distiller's grains in China is as a raw material for feed. However, due to the need to dry them, it will not only cause problems such as low efficiency, but also consume a lot of energy. [0003] Polyunsaturated fatty acid (polyunsaturated fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/6434C12P7/64C12R1/645
Inventor 欧莹秦宇杨海麟冯守帅张玲
Owner JIANGNAN UNIV
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