Method for producing baohuoside I through herba epimdii

A technology of epimedium and baohuoside, which is applied in the field of extraction of traditional Chinese medicine components, can solve the problems of cumbersome and difficult control, high concentration energy consumption, product damage, etc., and achieve the effects of simple process operation, easy separation and purification, and reduced product loss

Active Publication Date: 2014-12-24
XICHANG DANYANG BIOLOGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 1. Due to low-alcohol extraction, the extract contains a lot of water, high energy consumption for concentration, high concentration temperature and long time
Since the C-8 position of baohuoside I has a sensitive isopentenyl group, and other parts have relatively sensitive carbonyl and ketone groups, high temperature for a long time will greatly damage the product
At the same time, low-alcohol contains more water and brings out more impurities, which increases the difficulty of separation
The water liquid is left after the concentration of low-alcohol, which does not dissolve the target product baohuoside I, which affects the extraction effect
The addition of emulsifier Tween 80 only helps the proposed icariin to be hydrolyzed into baohuoside I and partly dissolves into water, while baohuoside I in plant tissues is difficult to extract, resulting in incomplete presentation
[0010] 2. The price of cellulase is high, and 5 kg per ton of raw materials is used, which is a large amount
Make this process product without cost advantage
4. Due to the above defects of the process (unclean extraction, many impurities brought out, and large product damage), the content of the extract is low, resulting in difficulty in product purification
It is cumbersome and difficult to control, and the cost is too high

Method used

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  • Method for producing baohuoside I through herba epimdii
  • Method for producing baohuoside I through herba epimdii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The method that epimedium ferment produces baohuoside I, it comprises the following steps:

[0060] S1. Take 40g of high-activity dry yeast and multiply in the culture medium for 10 hours. The expansion culture conditions are pH 6-7, temperature 25°C, static fermentation, and then add 2 times the amount of water to the volume of the expansion solution to obtain a culture solution; The medium is prepared by adding water to starch, the amount of starch is 500 times the weight of yeast (20kg), the weight ratio of water to starch added to the starch is 6:1, and the starch is wheat flour; highly active dry yeast It is "highly active dry yeast" stipulated in the industry standard QR / T1501-92 of the People's Republic of China, excellent grade or first grade product; fermentation power ≥ 750ml (CO 2 ).

[0061] S2. Mix the nutrient solution obtained in step S1 with 240 kg of dried epimedium pulverized to 10 to 20 meshes, detect that the content of icariin is 0.55%, stir evenl...

Embodiment 2

[0072] The method that epimedium ferment produces baohuoside I, it comprises the following steps:

[0073] S1. Take 300g of highly active dry yeast and multiply it in the culture medium for 8 hours. The expansion culture conditions are pH 6-7, temperature 35°C, static fermentation, and then add 2.5 times the amount of water according to the volume of the expansion solution to dilute to obtain a culture solution; The medium is prepared by adding water to starch, the amount of starch is 666.7 times the weight of the yeast (200kg), the weight ratio of water to starch added to the starch is 5:1, and the starch is wheat flour; highly active dry yeast It is "highly active dry yeast" stipulated in the industry standard QR / T1501-92 of the People's Republic of China, excellent grade or first grade product; fermentation power ≥ 750ml (CO 2 ).

[0074] S2. Mix the culture solution obtained in step S1 with 2.4 tons of dried epimedium dried raw materials crushed to 10-20 meshes, stir eve...

Embodiment 3

[0084] The method that epimedium ferment produces baohuoside I, it comprises the following steps:

[0085] S1. Take 40g of high-activity dry yeast and multiply in the culture medium for 12 hours. The expansion culture conditions are pH 6-7, temperature 15°C, static fermentation, and then add 2 times the amount of water according to the volume of the expansion solution to dilute to obtain a culture solution; The medium is prepared by adding water to starch, the amount of starch is 800 times the weight of the yeast, the weight ratio of water and starch added to the starch is 4:1, the starch is wheat flour; highly active dry yeast is Chinese people's "Highly active dry yeast" stipulated in the industry standard of the Republic QR / T1501-92, superior or first-class product; fermentation power ≥ 750ml (CO 2 ).

[0086] S2, mix the nutrient solution obtained in step S1 with 1000 kg of the fresh epimedium that is crushed to 10-20 meshes, stir evenly, the weight ratio of the fresh ep...

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Abstract

The invention discloses a method for producing baohuoside I through herba epimdii. The method comprises the following steps of: S1. expanding propagating and diluting saccharomyces cerevisiae in culture medium; S2. mixing and evenly stirring culture solution and herba epimdii, and carrying out close and static anaerobic fermentation in a fermentation tank; S3. when the herba epimdii is entirely hydrolyzed, extracting fermenting raw materials with an extraction solvent; S4. washing with extract dichloromethane, and extracting ethyl acetate; and S5. filtering ethyl acetate through an alumina column, then carrying out silica gel adsorption column chromatography to obtain pure baohuoside I. The method has the beneficial effects of being simple and convenient to operate, easy to implement, high in efficiency, fast, low in cost, high in yield, pollution-free by adopting microbial solid-state anaerobic fermentation to hydrolyze herba epimdii-containing raw materials, thus being suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to a process for extracting traditional Chinese medicine components, in particular to a method for producing baohuoside I by fermenting epimedium. Background technique [0002] Epimedium is the Berberidaceae plant Epimedium heart leaf, Epimedium brevicornum Maxim., Epimedium sagittatum (Sieb. et Zucc.) Maxim.), Epimedium pilose ( Epimedium pubescens Maxim.), Epimedium wushanense T.S. Ying, or the dry aerial part of Epimedium koreanum Nakai. Harvest in summer and autumn when the stems and leaves are lush, remove thick stems and impurities, and dry in the sun or in the shade. [0003] Epimedium is a traditional tonic Chinese medicine, which has various functions such as tonifying kidney yang, strengthening muscles and bones, and dispelling rheumatism. [0004] Baohuoside I (Baohuoside I), also known as Icarisid II, exists in a small amount in Wujiapi and Epimedium pilosa. Baohuoside I has stronger and wider pharmacological activi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/60C12R1/865
Inventor 蒋洪武杨红刘卓艳波拉提.马卡比力江发玉
Owner XICHANG DANYANG BIOLOGY TECH CO LTD
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